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OPA3 optic atrophy 3 (autosomal recessive, with chorea and spastic paraplegia) [Homo sapiens (human)]

Gene Symbol OPA3
Entrez Gene ID 80207
Full Name optic atrophy 3 (autosomal recessive, with chorea and spastic paraplegia)
Synonyms MGA3
General protein information
Preferred Names
optic atrophy 3 protein
Names
optic atrophy 3 protein
Optic atrophy 3 (Iraqi-Jewish 'optic atrophy plus')
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

19

19q13.32

Summary The mouse ortholog of this protein co-purifies with the mitochondrial inner membrane. Mutations in this gene have been shown to result in 3-methylglutaconic aciduria type III and autosomal dominant optic atrophy and cataract. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]. lac of sum
Disorder MIM:

606580

Disorder Html: 3-methylglutaconic aciduria, type III, 258501 (3); Optic atrophy
CloneID RefSeq Accession Definition **Vector *Turnaround time Price
OHu72660 XM_011527348 PREDICTED: Homo sapiens optic atrophy 3 (autosomal recessive, with chorea and spastic paraplegia) (OPA3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu52502 XM_006723403 PREDICTED: Homo sapiens optic atrophy 3 (autosomal recessive, with chorea and spastic paraplegia) (OPA3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu52502 XM_005259278 PREDICTED: Homo sapiens optic atrophy 3 (autosomal recessive, with chorea and spastic paraplegia) (OPA3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu19423 NM_001017989 Homo sapiens optic atrophy 3 (autosomal recessive, with chorea and spastic paraplegia) (OPA3), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu23186 NM_025136 Homo sapiens optic atrophy 3 (autosomal recessive, with chorea and spastic paraplegia) (OPA3), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1-C-(k)DYK after clone is added to cart.



CloneID OHu72660D
Sequence Information ORF Nucleotide Sequence (Length: 384bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product optic atrophy 3 protein isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011109.17) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
ATGCGGACCA AAATGCGCAT CATGGGTTTC AATGCCGCTG CCATCAAGCC GCTGAACGAG 
GGTGCAGCCG CCGAGCTGGG CGCGGAGCTG CTGGGCGAGG GCATCATCTT CATCACCGCC
TGCAGCTGCC TGATGCTGGA GTATTGGCGC CACCAGTTGC AGCAGCGCCG CAAGGAAAAG
GAGCGACGTG TTGCCAGGGA GGCGCTGCGG GGCGAGGTGG GCCACTTGGG GCTGGCGCTC
GAGGAGTTGC AGGCGCAGGT GCAGGCGACG TCGACGCAGC TCGCCCTGGA GGAGCTGCGC
GCTCAGCTGC AGGAGGTGCG AGCCCACCTC TGCCTCCGAG ACCCGCCGCC TGCACCCCCA
GTTGCGCCGG CGTCCGAGAA ATAG
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_011525650.1
CDS290..673
Misc Feature(1)290..547(+)
Translation
Position Chain Variation Link
1 1 a, c, t dbSNP:539476253
2 2 c, t dbSNP:761112615
3 3 a, c dbSNP:775935805
4 4 c, t dbSNP:771575785
7 7 -, a dbSNP:773090739
11 11 c, t dbSNP:745326007
15 15 c, g dbSNP:778382919
21 21 c, t dbSNP:770554253
26 26 c, g dbSNP:748938403
34 34 a, g dbSNP:45551337
35 35 a, t dbSNP:755889145
38 38 c, t dbSNP:752709458
39 39 -, ct dbSNP:567190354
44 44 a, g dbSNP:116426072
45 45 c, g dbSNP:538096384
60 60 c, g dbSNP:62111684
65 65 a, g dbSNP:750484828
72 72 c, t dbSNP:113539314
94 94 a, g dbSNP:529074692
108 108 a, g, t dbSNP:551975047
113 113 c, t dbSNP:546655244
128 128 c, t dbSNP:532811942
141 141 a, c dbSNP:564075556
148 148 a, g dbSNP:544516313
185 185 c, t dbSNP:184511563
194 194 c, g dbSNP:560090853
215 215 -, a dbSNP:35374071
239 239 c, g dbSNP:542051236
264 264 -, ac dbSNP:34283843
280 280 c, g dbSNP:771376809
288 288 a, t dbSNP:749841549
293 293 a, c, g dbSNP:755659368
298 298 c, g dbSNP:747666099
304 304 a, g dbSNP:780748496
306 306 c, g dbSNP:754626320
308 308 a, g dbSNP:141142300
310 310 c, g dbSNP:779844856
313 313 a, g dbSNP:758125545
314 314 a, g dbSNP:140959406
334 334 c, t dbSNP:765146461
338 338 a, c dbSNP:761806800
347 347 a, c, g, t dbSNP:759861826
348 348 a, t dbSNP:759523944
351 351 -, g dbSNP:779350552
357 357 c, t dbSNP:774673258
360 360 c, t dbSNP:771423936
362 362 g, t dbSNP:763348163
369 369 g, t dbSNP:761801627
371 371 a, g dbSNP:200304633
372 372 c, t dbSNP:748656480
386 386 g, t dbSNP:780614894
388 388 c, g dbSNP:202205093
389 389 a, g dbSNP:746670368
395 395 a, g dbSNP:779625614
398 398 -, ttc dbSNP:755084544
407 407 a, g dbSNP:758033801
412 412 c, t dbSNP:750286310
430 430 a, g dbSNP:778741761
432 432 a, g dbSNP:757195041
434 434 c, t dbSNP:753763361
437 437 a, c dbSNP:568889742
439 439 c, t dbSNP:532551321
444 444 a, c dbSNP:751841657
446 446 g, t dbSNP:766762022
463 463 a, g dbSNP:763259776
469 469 c, g dbSNP:773488519
473 473 a, c, t dbSNP:762247018
476 476 c, g dbSNP:777155225
484 484 c, t dbSNP:768169499
485 485 -, a dbSNP:753909971
491 491 a, g dbSNP:746582143
495 495 c, g, t dbSNP:771753477
510 510 g, t dbSNP:745514950
511 511 c, t dbSNP:778599826
513 513 a, t dbSNP:757040753
517 517 c, g dbSNP:749184660
520 520 a, g dbSNP:777706172
525 525 c, t dbSNP:756103311
526 526 c, g dbSNP:751753401
530 530 a, g dbSNP:766535284
536 536 c, t dbSNP:535476484
543 543 c, g dbSNP:750607594
544 544 a, g dbSNP:566450630
545 545 c, t dbSNP:762180740
546 546 a, t dbSNP:201888844
547 547 a, g dbSNP:764652387
551 551 c, t dbSNP:374175877
552 552 a, g dbSNP:761140317
558 558 c, t dbSNP:371097138
571 571 c, t dbSNP:771526677
575 575 -, c dbSNP:780299639
581 581 a, g dbSNP:745540793
584 584 c, g, t dbSNP:376630127
585 585 g, t dbSNP:749162539
586 586 g, t dbSNP:777702532
588 588 a, g dbSNP:539903027
589 589 c, t dbSNP:748075455
590 590 c, g dbSNP:547349748
605 605 g, t dbSNP:758510921
609 609 c, g dbSNP:750612755
613 613 c, t dbSNP:373469627
616 616 c, t dbSNP:201078416
634 634 g, t dbSNP:757418839
644 644 c, t dbSNP:201023208
645 645 c, t dbSNP:764421231
646 646 c, g dbSNP:761195604
649 649 a, t dbSNP:368970746
651 651 a, t dbSNP:767022079
655 655 g, t dbSNP:759025249
663 663 c, t dbSNP:773719228
665 665 a, g dbSNP:770679537
666 666 -, a dbSNP:756360957
667 667 g, t dbSNP:748857760
672 672 a, g dbSNP:375580763
675 675 -, agg dbSNP:750700198
675 675 a, t dbSNP:190812374
686 686 a, c dbSNP:769663941
687 687 c, t dbSNP:530916858
688 688 c, t dbSNP:781207627
691 691 a, g dbSNP:768465514
715 715 a, g dbSNP:763313333
718 718 a, g dbSNP:746029215
737 737 c, t dbSNP:775820623
739 739 a, g dbSNP:185891858
745 745 c, t dbSNP:541954840
746 746 g, t dbSNP:770344909
765 765 a, c dbSNP:749610366
786 786 c, g dbSNP:528516281
793 793 c, g dbSNP:559307674
801 801 c, g dbSNP:759936639
813 813 a, g dbSNP:540153905
817 817 c, t dbSNP:528100454
834 834 c, t dbSNP:577488389
863 863 a, g dbSNP:73568948
870 870 c, t dbSNP:544356178
883 883 a, g dbSNP:575339012
894 894 g, t dbSNP:555489004
900 900 -, a dbSNP:71338780
902 902 -, aag dbSNP:778914148
903 903 -, a dbSNP:71338779
904 904 g, t dbSNP:535182496
933 933 a, c dbSNP:563379703
934 934 c, t dbSNP:566488233
983 983 a, c dbSNP:552974949
986 986 c, g dbSNP:543389826
992 992 a, g dbSNP:540138385
994 994 c, t dbSNP:571115232
1004 1004 g, t dbSNP:182553559
1046 1046 a, g dbSNP:531181521
1062 1062 c, g dbSNP:771405313
1106 1106 -, c dbSNP:376254737
1114 1114 a, g dbSNP:575993357
1116 1116 c, t dbSNP:747645538
1130 1130 a, c dbSNP:568538750
1141 1141 c, t dbSNP:557652872
1170 1170 c, t dbSNP:544919803
1224 1224 c, t dbSNP:528532265
1233 1233 -, a dbSNP:150558316
1248 1248 a, g dbSNP:543505586
1264 1264 c, g dbSNP:369033022
1290 1290 a, g dbSNP:559344315
1298 1298 g, t dbSNP:545747717
1312 1312 c, t dbSNP:533069420
1320 1320 a, c dbSNP:564448491
1323 1323 c, t dbSNP:544179501
1337 1337 c, t dbSNP:575428147
1381 1381 a, c, g, t dbSNP:7259834
1390 1390 g, t dbSNP:191039825
1407 1407 c, g dbSNP:768132755
1449 1449 c, g dbSNP:572948185
1548 1548 a, g dbSNP:748979866
1614 1614 g, t dbSNP:779667115
1630 1630 c, t dbSNP:186186357
1631 1631 c, g dbSNP:539475374
1632 1632 -, g dbSNP:34997904
1673 1673 c, t dbSNP:755874224
1677 1677 c, t dbSNP:750164782
1715 1715 c, t dbSNP:115623295
1720 1720 -, tcct dbSNP:781432805
1724 1724 g, t dbSNP:557682873
1752 1752 a, g dbSNP:537335238
1810 1810 a, c dbSNP:150986093
1849 1849 c, t dbSNP:548446096
1873 1873 a, g dbSNP:534811674
1875 1875 a, g dbSNP:565799330
1913 1913 c, t dbSNP:142545664
1922 1922 a, c dbSNP:2270199
1933 1933 a, g dbSNP:751646789
1948 1948 a, g dbSNP:757166355
1955 1955 g, t dbSNP:563527167
1987 1987 a, g dbSNP:751517859
2024 2024 c, t dbSNP:550864481
2035 2035 aa, gc dbSNP:386809765
2035 2035 a, g dbSNP:116329407
2036 2036 a, c dbSNP:116046357
2072 2072 c, g dbSNP:542013440
2108 2108 a, g dbSNP:61267572
2139 2139 c, t dbSNP:148434999
2155 2155 a, t dbSNP:545911554
2163 2163 c, t dbSNP:576813495
2186 2186 c, t dbSNP:556643014
2265 2265 c, t dbSNP:188814360
2268 2268 a, t dbSNP:575002396
2279 2279 a, g dbSNP:117210247
2285 2285 a, g dbSNP:534920333
2295 2295 c, t dbSNP:7254594
2305 2305 -, aaaataaaaaaa dbSNP:58897048
2315 2315 a, g dbSNP:552296555
2321 2321 -, aaaaaa dbSNP:111617769
2326 2326 -, a dbSNP:774448358
2329 2329 a, g dbSNP:373345127
2350 2350 -, tagagtgg dbSNP:558049297
2355 2355 c, t dbSNP:536178784
2356 2356 c, g, t dbSNP:186391311
2435 2435 a, c, t dbSNP:370011307
2453 2453 -, c dbSNP:535234682
2460 2460 c, t dbSNP:530851714
2481 2481 a, g dbSNP:559007319
2484 2484 -, g dbSNP:373351844
2495 2495 a, g dbSNP:548482924
2509 2509 -, cct dbSNP:546820850
2538 2538 c, g dbSNP:568831117
2569 2569 a, g dbSNP:149162609
2576 2576 c, t dbSNP:3810334
2583 2583 c, g dbSNP:545923732
2585 2585 a, g dbSNP:532062531
2599 2599 a, g dbSNP:563316633
2618 2618 a, g dbSNP:773677764
2626 2626 c, t dbSNP:543014778
2636 2636 -, agg dbSNP:767349870
2641 2641 a, g dbSNP:761377589
2696 2696 c, g dbSNP:574356806
2717 2717 a, g dbSNP:112356002
2719 2719 -, c dbSNP:763141150
2719 2719 c, t dbSNP:768222735
2789 2789 a, g dbSNP:748736827
2807 2807 g, t dbSNP:1051907
2827 2827 c, t dbSNP:189567141
2833 2833 c, t dbSNP:10995

Target ORF information:

RefSeq Version XM_011527348
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens optic atrophy 3 (autosomal recessive, with chorea and spastic paraplegia) (OPA3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_011527348

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGCGGACCA AAATGCGCAT CATGGGTTTC AATGCCGCTG CCATCAAGCC GCTGAACGAG 
GGTGCAGCCG CCGAGCTGGG CGCGGAGCTG CTGGGCGAGG GCATCATCTT CATCACCGCC
TGCAGCTGCC TGATGCTGGA GTATTGGCGC CACCAGTTGC AGCAGCGCCG CAAGGAAAAG
GAGCGACGTG TTGCCAGGGA GGCGCTGCGG GGCGAGGTGG GCCACTTGGG GCTGGCGCTC
GAGGAGTTGC AGGCGCAGGT GCAGGCGACG TCGACGCAGC TCGCCCTGGA GGAGCTGCGC
GCTCAGCTGC AGGAGGTGCG AGCCCACCTC TGCCTCCGAG ACCCGCCGCC TGCACCCCCA
GTTGCGCCGG CGTCCGAGAA ATAG

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu52502D
Sequence Information ORF Nucleotide Sequence (Length: 381bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product optic atrophy 3 protein isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011109.17) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578834945. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
ATGCGGACCA AGATGCGCAT CATGGGCTTC CGGGGCACGG TCATCAAGCC GCTGAACGAG 
GAGGCGGCAG CTGAGCTGGG CGCAGAGCTG CTGGGCGAAG CCACCATCTT CATCGTGGGC
GGCGGCTGCC TAGTGCTGGA GTACTGGCGC CACCAGGCGC AGCAGCGCCA CAAGGAGGAG
GAGCAGCGTG CTGCCTGGAA CGCGCTGCGG GACGAGGTGG GCCACCTGGC GCTGGCGCTG
GAAGCGCTGC AGGCGCAGGT GCAGGCGGCG CCGCCACAGG GCGCCCTGGA GGAACTGCGC
ACAGAGCTGC AAGAGGTGCG CGCCCAGCTC TGCAATCCCG GCCGGTCCGC TTCCCACGCA
GTGCCTGCGT CCAAGAAATTA G
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_006723466.1
CDS236..616
Misc Feature(1)236..457(+)
Translation
Position Chain Variation Link
1 1 c, t dbSNP:757060614
30 30 a, c dbSNP:753632531
31 31 a, g dbSNP:777618441
48 48 a, g dbSNP:756044697
56 56 c, t dbSNP:752657560
57 57 a, g dbSNP:766584469
59 59 c, g dbSNP:763083098
65 65 c, t dbSNP:201438203
71 71 a, g dbSNP:553414785
173 173 a, c dbSNP:539845804
223 223 c, t dbSNP:769477086
228 228 a, g dbSNP:747890876
238 238 a, g dbSNP:781119036
239 239 a, c dbSNP:748653611
240 240 c, g dbSNP:755008161
241 241 g, t dbSNP:746924502
243 243 c, t dbSNP:778885990
254 254 a, c dbSNP:757323736
266 266 c, t dbSNP:754011378
267 267 a, g dbSNP:764320249
270 270 g, t dbSNP:144382886
273 273 c, t dbSNP:753129998
277 277 c, t dbSNP:539986269
282 282 a, c dbSNP:760083523
284 284 c, t dbSNP:774790473
285 285 c, t dbSNP:143027616
286 286 a, g dbSNP:762454431
292 292 c, t dbSNP:772750356
300 300 c, t dbSNP:769541845
307 307 c, t dbSNP:3826860
316 316 c, t dbSNP:776619481
317 317 a, g dbSNP:186796646
324 324 a, t dbSNP:746977962
325 325 c, g dbSNP:199692583
328 328 a, g dbSNP:757297634
329 329 a, g dbSNP:749444775
344 344 -, ttc dbSNP:761741716
349 349 c, g, t dbSNP:369545089
350 350 a, g dbSNP:752893027
353 353 a, g dbSNP:80356524
359 359 g, t dbSNP:767889585
365 365 c, t dbSNP:755295420
367 367 a, g dbSNP:755689286
379 379 c, t dbSNP:752010426
382 382 c, g dbSNP:766847630
383 383 c, t dbSNP:763353585
389 389 c, g dbSNP:80356525
392 392 a, g dbSNP:750013718
393 393 c, t dbSNP:764633370
396 396 -, agcagcgccacaaggagg dbSNP:80356526
409 409 a, g dbSNP:761583193
412 412 a, g dbSNP:146349367
413 413 a, g dbSNP:142467378
427 427 c, t dbSNP:760489108
428 428 c, g, t dbSNP:772116334
430 430 c, t dbSNP:745763712
431 431 a, t dbSNP:375471439
435 435 a, g dbSNP:769813697
442 442 g, t dbSNP:748397260
445 445 a, g dbSNP:781320613
448 448 a, c dbSNP:755348694
455 455 a, g dbSNP:575871533
461 461 -, acc dbSNP:774167136
465 465 a, c dbSNP:780275755
471 471 -, a dbSNP:768199119
471 471 a, c dbSNP:758982194
476 476 -, cgctgg dbSNP:748815011
480 480 c, g dbSNP:372640359
481 481 g, t dbSNP:750848548
486 486 a, c dbSNP:372161100
488 488 a, g dbSNP:201574732
491 491 c, t dbSNP:28937899
500 500 a, g dbSNP:535683352
503 503 c, g dbSNP:567096759
504 504 c, t dbSNP:553598004
505 505 a, g dbSNP:745373208
507 507 c, g, t dbSNP:775398472
508 508 a, g dbSNP:45470595
518 518 g, t dbSNP:771739856
520 520 c, t dbSNP:759536853
526 526 a, g dbSNP:529981150
534 534 g, t dbSNP:539965360
546 546 -, aagaggtgc dbSNP:745544229
547 547 a, g dbSNP:570940748
552 552 c, t dbSNP:748168313
554 554 -, cttaact dbSNP:769486266
554 554 c, t dbSNP:137978109
556 556 c, t dbSNP:768932301
560 560 a, c dbSNP:747167154
563 563 c, t dbSNP:780492515
570 570 a, g dbSNP:558412882
578 578 c, g dbSNP:750946088
579 579 a, g dbSNP:779505447
583 583 c, t dbSNP:757829968
584 584 a, g dbSNP:374639297
593 593 a, g dbSNP:371832021
602 602 a, g dbSNP:755841329
604 604 a, g dbSNP:752240099
608 608 a, g dbSNP:767372876
610 610 -, gaaa dbSNP:778074016
613 613 a, g dbSNP:140105522
614 614 g, t dbSNP:780840710
616 616 a, g dbSNP:774281852
625 625 a, t dbSNP:201697957
626 626 g, t dbSNP:762873253
627 627 -, t dbSNP:35625615
634 634 c, t dbSNP:776730325
635 635 c, t dbSNP:768697570
643 643 a, g dbSNP:747289326
644 644 a, g dbSNP:367663395
647 647 a, c dbSNP:775509581
650 650 a, g dbSNP:772500238
652 652 c, t dbSNP:562456005
654 654 a, g dbSNP:746276271
655 655 c, t dbSNP:779419503
657 657 a, t dbSNP:757882898
659 659 c, t dbSNP:749792566
664 664 c, t dbSNP:146126868
676 676 c, t dbSNP:529172414
680 680 c, t dbSNP:559943742
696 696 g, t dbSNP:540023428
701 701 c, t dbSNP:532881285
725 725 a, g dbSNP:563811459
768 768 a, g dbSNP:3826861
842 842 c, g dbSNP:575138678
858 858 c, g dbSNP:144259826
860 860 c, t dbSNP:542553691
861 861 c, t dbSNP:139637265
888 888 a, g dbSNP:7246349
936 936 c, t dbSNP:541330507
942 942 -, gt dbSNP:571672143
978 978 c, t dbSNP:73942919
1014 1014 g, t dbSNP:183851663
1023 1023 c, t dbSNP:537060236
1038 1038 c, t dbSNP:116977551
1040 1040 c, g dbSNP:548266881
1104 1104 c, g dbSNP:780231275
1148 1148 -, g dbSNP:563314271
1156 1156 g, t dbSNP:149360920
1165 1165 a, g dbSNP:566396414
1193 1193 c, t dbSNP:112654759
1195 1195 c, g dbSNP:532917256
1211 1211 c, t dbSNP:539993852
1254 1254 a, t dbSNP:150575877
1257 1257 c, t dbSNP:550694350
1293 1293 c, t dbSNP:560749495
1309 1309 c, t dbSNP:530521545
1314 1314 a, g dbSNP:561748985
1329 1329 c, t dbSNP:143735864
1338 1338 a, g dbSNP:542591243
1383 1383 a, g dbSNP:138079196
1408 1408 a, g dbSNP:560058795
1419 1419 a, g dbSNP:773462602
1453 1453 c, t dbSNP:764268778
1454 1454 a, g dbSNP:546421510
1457 1457 c, t dbSNP:577472780
1510 1510 c, t dbSNP:575283920
1526 1526 a, g dbSNP:192099573
1555 1555 a, c dbSNP:537579700
1561 1561 c, t dbSNP:574822690
1572 1572 a, g dbSNP:554964579
1587 1587 -, a dbSNP:34303121

Target ORF information:

RefSeq Version XM_006723403
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens optic atrophy 3 (autosomal recessive, with chorea and spastic paraplegia) (OPA3), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_006723403

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGCGGACCA AGATGCGCAT CATGGGCTTC CGGGGCACGG TCATCAAGCC GCTGAACGAG 
GAGGCGGCAG CTGAGCTGGG CGCAGAGCTG CTGGGCGAAG CCACCATCTT CATCGTGGGC
GGCGGCTGCC TAGTGCTGGA GTACTGGCGC CACCAGGCGC AGCAGCGCCA CAAGGAGGAG
GAGCAGCGTG CTGCCTGGAA CGCGCTGCGG GACGAGGTGG GCCACCTGGC GCTGGCGCTG
GAAGCGCTGC AGGCGCAGGT GCAGGCGGCG CCGCCACAGG GCGCCCTGGA GGAACTGCGC
ACAGAGCTGC AAGAGGTGCG CGCCCAGCTC TGCAATCCCG GCCGGTCCGC TTCCCACGCA
GTGCCTGCGT CCAAGAAATTA G

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu52502D
Sequence Information ORF Nucleotide Sequence (Length: 381bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product optic atrophy 3 protein isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011109.17) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530417294. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
ATGCGGACCA AGATGCGCAT CATGGGCTTC CGGGGCACGG TCATCAAGCC GCTGAACGAG 
GAGGCGGCAG CTGAGCTGGG CGCAGAGCTG CTGGGCGAAG CCACCATCTT CATCGTGGGC
GGCGGCTGCC TAGTGCTGGA GTACTGGCGC CACCAGGCGC AGCAGCGCCA CAAGGAGGAG
GAGCAGCGTG CTGCCTGGAA CGCGCTGCGG GACGAGGTGG GCCACCTGGC GCTGGCGCTG
GAAGCGCTGC AGGCGCAGGT GCAGGCGGCG CCGCCACAGG GCGCCCTGGA GGAACTGCGC
ACAGAGCTGC AAGAGGTGCG CGCCCAGCTC TGCAATCCCG GCCGGTCCGC TTCCCACGCA
GTGCCTGCGT CCAAGAAATTA G
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_005259335.1
CDS104..484
Misc Feature(1)104..325(+)
Translation
Position Chain Variation Link
8 8 c, g dbSNP:560090853
29 29 -, a dbSNP:35374071
53 53 c, g dbSNP:542051236
78 78 -, ac dbSNP:34283843
91 91 c, t dbSNP:769477086
96 96 a, g dbSNP:747890876
106 106 a, g dbSNP:781119036
107 107 a, c dbSNP:748653611
108 108 c, g dbSNP:755008161
109 109 g, t dbSNP:746924502
111 111 c, t dbSNP:778885990
122 122 a, c dbSNP:757323736
134 134 c, t dbSNP:754011378
135 135 a, g dbSNP:764320249
138 138 g, t dbSNP:144382886
141 141 c, t dbSNP:753129998
145 145 c, t dbSNP:539986269
150 150 a, c dbSNP:760083523
152 152 c, t dbSNP:774790473
153 153 c, t dbSNP:143027616
154 154 a, g dbSNP:762454431
160 160 c, t dbSNP:772750356
168 168 c, t dbSNP:769541845
175 175 c, t dbSNP:3826860
184 184 c, t dbSNP:776619481
185 185 a, g dbSNP:186796646
192 192 a, t dbSNP:746977962
193 193 c, g dbSNP:199692583
196 196 a, g dbSNP:757297634
197 197 a, g dbSNP:749444775
212 212 -, ttc dbSNP:761741716
217 217 c, g, t dbSNP:369545089
218 218 a, g dbSNP:752893027
221 221 a, g dbSNP:80356524
227 227 g, t dbSNP:767889585
233 233 c, t dbSNP:755295420
235 235 a, g dbSNP:755689286
247 247 c, t dbSNP:752010426
250 250 c, g dbSNP:766847630
251 251 c, t dbSNP:763353585
257 257 c, g dbSNP:80356525
260 260 a, g dbSNP:750013718
261 261 c, t dbSNP:764633370
264 264 -, agcagcgccacaaggagg dbSNP:80356526
277 277 a, g dbSNP:761583193
280 280 a, g dbSNP:146349367
281 281 a, g dbSNP:142467378
295 295 c, t dbSNP:760489108
296 296 c, g, t dbSNP:772116334
298 298 c, t dbSNP:745763712
299 299 a, t dbSNP:375471439
303 303 a, g dbSNP:769813697
310 310 g, t dbSNP:748397260
313 313 a, g dbSNP:781320613
316 316 a, c dbSNP:755348694
323 323 a, g dbSNP:575871533
329 329 -, acc dbSNP:774167136
333 333 a, c dbSNP:780275755
339 339 -, a dbSNP:768199119
339 339 a, c dbSNP:758982194
344 344 -, cgctgg dbSNP:748815011
348 348 c, g dbSNP:372640359
349 349 g, t dbSNP:750848548
354 354 a, c dbSNP:372161100
356 356 a, g dbSNP:201574732
359 359 c, t dbSNP:28937899
368 368 a, g dbSNP:535683352
371 371 c, g dbSNP:567096759
372 372 c, t dbSNP:553598004
373 373 a, g dbSNP:745373208
375 375 c, g, t dbSNP:775398472
376 376 a, g dbSNP:45470595
386 386 g, t dbSNP:771739856
388 388 c, t dbSNP:759536853
394 394 a, g dbSNP:529981150
402 402 g, t dbSNP:539965360
414 414 -, aagaggtgc dbSNP:745544229
415 415 a, g dbSNP:570940748
420 420 c, t dbSNP:748168313
422 422 -, cttaact dbSNP:769486266
422 422 c, t dbSNP:137978109
424 424 c, t dbSNP:768932301
428 428 a, c dbSNP:747167154
431 431 c, t dbSNP:780492515
438 438 a, g dbSNP:558412882
446 446 c, g dbSNP:750946088
447 447 a, g dbSNP:779505447
451 451 c, t dbSNP:757829968
452 452 a, g dbSNP:374639297
461 461 a, g dbSNP:371832021
470 470 a, g dbSNP:755841329
472 472 a, g dbSNP:752240099
476 476 a, g dbSNP:767372876
478 478 -, gaaa dbSNP:778074016
481 481 a, g dbSNP:140105522
482 482 g, t dbSNP:780840710
484 484 a, g dbSNP:774281852
493 493 a, t dbSNP:201697957
494 494 g, t dbSNP:762873253
495 495 -, t dbSNP:35625615
502 502 c, t dbSNP:776730325
503 503 c, t dbSNP:768697570
511 511 a, g dbSNP:747289326
512 512 a, g dbSNP:367663395
515 515 a, c dbSNP:775509581
518 518 a, g dbSNP:772500238
520 520 c, t dbSNP:562456005
522 522 a, g dbSNP:746276271
523 523 c, t dbSNP:779419503
525 525 a, t dbSNP:757882898
527 527 c, t dbSNP:749792566
532 532 c, t dbSNP:146126868
544 544 c, t dbSNP:529172414
548 548 c, t dbSNP:559943742
564 564 g, t dbSNP:540023428
569 569 c, t dbSNP:532881285
593 593 a, g dbSNP:563811459
636 636 a, g dbSNP:3826861
710 710 c, g dbSNP:575138678
726 726 c, g dbSNP:144259826
728 728 c, t dbSNP:542553691
729 729 c, t dbSNP:139637265
756 756 a, g dbSNP:7246349
804 804 c, t dbSNP:541330507
810 810 -, gt dbSNP:571672143
846 846 c, t dbSNP:73942919
882 882 g, t dbSNP:183851663
891 891 c, t dbSNP:537060236
906 906 c, t dbSNP:116977551
908 908 c, g dbSNP:548266881
972 972 c, g dbSNP:780231275
1016 1016 -, g dbSNP:563314271
1024 1024 g, t dbSNP:149360920
1033 1033 a, g dbSNP:566396414
1061 1061 c, t dbSNP:112654759
1063 1063 c, g dbSNP:532917256
1079 1079 c, t dbSNP:539993852
1122 1122 a, t dbSNP:150575877
1125 1125 c, t dbSNP:550694350
1161 1161 c, t dbSNP:560749495
1177 1177 c, t dbSNP:530521545
1182 1182 a, g dbSNP:561748985
1197 1197 c, t dbSNP:143735864
1206 1206 a, g dbSNP:542591243
1251 1251 a, g dbSNP:138079196
1276 1276 a, g dbSNP:560058795
1287 1287 a, g dbSNP:773462602
1321 1321 c, t dbSNP:764268778
1322 1322 a, g dbSNP:546421510
1325 1325 c, t dbSNP:577472780
1378 1378 c, t dbSNP:575283920
1394 1394 a, g dbSNP:192099573
1423 1423 a, c dbSNP:537579700
1429 1429 c, t dbSNP:574822690
1440 1440 a, g dbSNP:554964579
1455 1455 -, a dbSNP:34303121

Target ORF information:

RefSeq Version XM_005259278
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens optic atrophy 3 (autosomal recessive, with chorea and spastic paraplegia) (OPA3), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_005259278

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGCGGACCA AGATGCGCAT CATGGGCTTC CGGGGCACGG TCATCAAGCC GCTGAACGAG 
GAGGCGGCAG CTGAGCTGGG CGCAGAGCTG CTGGGCGAAG CCACCATCTT CATCGTGGGC
GGCGGCTGCC TAGTGCTGGA GTACTGGCGC CACCAGGCGC AGCAGCGCCA CAAGGAGGAG
GAGCAGCGTG CTGCCTGGAA CGCGCTGCGG GACGAGGTGG GCCACCTGGC GCTGGCGCTG
GAAGCGCTGC AGGCGCAGGT GCAGGCGGCG CCGCCACAGG GCGCCCTGGA GGAACTGCGC
ACAGAGCTGC AAGAGGTGCG CGCCCAGCTC TGCAATCCCG GCCGGTCCGC TTCCCACGCA
GTGCCTGCGT CCAAGAAATTA G

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu19423D
Sequence Information ORF Nucleotide Sequence (Length: 543bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product optic atrophy 3 protein isoform a
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB034102.1, BC047316.1 and AC006117.1. This sequence is a reference standard in the RefSeqGene project. On Aug 22, 2007 this sequence version replaced gi:63055069. Summary: The mouse ortholog of this protein co-purifies with the mitochondrial inner membrane. Mutations in this gene have been shown to result in 3-methylglutaconic aciduria type III and autosomal dominant optic atrophy and cataract. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]. Transcript Variant: This variant (1) uses an alternate splice site resulting in a distinct 3' coding region and 3' UTR, compared to variant 2. The resulting isoform (a) has a longer and distinct C-terminus, compared to isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 18614015, 12865426; reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: BC047316.1, AK098798.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
ATGGTGGTGG GCGCGTTCCC TATGGCGAAG CTGCTATACT TGGGCATCCG GCAGGTCAGC 
AAGCCGCTTG CCAACCGTAT TAAGGAGGCC GCCCGCCGAA GCGAGTTCTT CAAGACCTAT
ATCTGCCTCC CGCCGGCTCA ACTGTACCAC TGGCTGGAGA TGCGGACCAA AATGCGCATC
ATGGGTTTCA ATGCCGCTGC CATCAAGCCG CTGAACGAGG GTGCAGCCGC CGAGCTGGGC
GCGGAGCTGC TGGGCGAGGG CATCATCTTC ATCACCGCCT GCAGCTGCCT GATGCTGGAG
TATTGGCGCC ACCAGTTGCA GCAGCGCCGC AAGGAAAAGG AGCGACGTGT TGCCAGGGAG
GCGCTGCGGG GCGAGGTGGG CCACTTGGGG CTGGCGCTCG AGGAGTTGCA GGCGCAGGTG
CAGGCGACGT CGACGCAGCT CGCCCTGGAG GAGCTGCGCG CTCAGCTGCA GGAGGTGCGA
GCCCACCTCT GCCTCCGAGA CCCGCCGCCT GCACCCCCAG TTGCGCCGGC GTCCGAGAAA
TAG
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_001017989.2
CDS101..643
Misc Feature(1)119..517(+)
Exon (1)1..242
Gene:OPA3
Gene Synonym:
Exon (2)243..1932
Gene:OPA3
Gene Synonym:
Translation
Position Chain Variation Link
5 5 a, g, t dbSNP:566870092
10 10 c, t dbSNP:546632526
15 15 a, g dbSNP:73570932
16 16 g, t dbSNP:571483175
26 26 a, g dbSNP:45598532
30 30 c, g dbSNP:531556421
31 31 a, g dbSNP:569038733
35 35 a, g dbSNP:549109182
36 36 g, t dbSNP:528949164
42 42 a, t dbSNP:560188551
45 45 a, g dbSNP:539890732
52 52 a, g dbSNP:771334307
55 55 c, t dbSNP:749517349
61 61 a, c, g dbSNP:770257059
63 63 a, g dbSNP:45527139
64 64 a, g dbSNP:781509098
66 66 c, t dbSNP:754517296
71 71 a, g dbSNP:746562214
75 75 c, t dbSNP:200159837
77 77 c, g, t dbSNP:750059099
79 79 c, t dbSNP:201065312
84 84 a, c dbSNP:757005901
89 89 a, g dbSNP:201864058
90 90 a, c dbSNP:763882469
93 93 a, c dbSNP:370143397
94 94 c, g, t dbSNP:199590627
97 97 c, g dbSNP:774359394
112 112 c, t dbSNP:766535154
113 113 a, g dbSNP:749185315
115 115 c, g dbSNP:763315476
120 120 c, t dbSNP:373427314
121 121 c, t dbSNP:770310018
133 133 g, t dbSNP:148805518
134 134 c, g dbSNP:200742525
136 136 a, g dbSNP:769113318
139 139 c, t dbSNP:374843726
142 142 a, g dbSNP:779592921
143 143 c, g dbSNP:771522612
147 147 a, t dbSNP:745552499
148 148 a, c dbSNP:778472683
165 165 c, t dbSNP:757060614
194 194 a, c dbSNP:753632531
195 195 a, g dbSNP:777618441
212 212 a, g dbSNP:756044697
220 220 c, t dbSNP:752657560
221 221 a, g dbSNP:766584469
223 223 c, g dbSNP:763083098
229 229 c, t dbSNP:201438203
235 235 a, g dbSNP:553414785
250 250 c, g dbSNP:771376809
258 258 a, t dbSNP:749841549
263 263 a, c, g dbSNP:755659368
268 268 c, g dbSNP:747666099
274 274 a, g dbSNP:780748496
276 276 c, g dbSNP:754626320
278 278 a, g dbSNP:141142300
280 280 c, g dbSNP:779844856
283 283 a, g dbSNP:758125545
284 284 a, g dbSNP:140959406
304 304 c, t dbSNP:765146461
308 308 a, c dbSNP:761806800
317 317 a, c, g, t dbSNP:759861826
318 318 a, t dbSNP:759523944
321 321 -, g dbSNP:779350552
327 327 c, t dbSNP:774673258
330 330 c, t dbSNP:771423936
332 332 g, t dbSNP:763348163
339 339 g, t dbSNP:761801627
341 341 a, g dbSNP:200304633
342 342 c, t dbSNP:748656480
356 356 g, t dbSNP:780614894
358 358 c, g dbSNP:202205093
359 359 a, g dbSNP:746670368
365 365 a, g dbSNP:779625614
368 368 -, ttc dbSNP:755084544
377 377 a, g dbSNP:758033801
382 382 c, t dbSNP:750286310
400 400 a, g dbSNP:778741761
402 402 a, g dbSNP:757195041
404 404 c, t dbSNP:753763361
407 407 a, c dbSNP:568889742
409 409 c, t dbSNP:532551321
414 414 a, c dbSNP:751841657
416 416 g, t dbSNP:766762022
433 433 a, g dbSNP:763259776
439 439 c, g dbSNP:773488519
443 443 a, c, t dbSNP:762247018
446 446 c, g dbSNP:777155225
454 454 c, t dbSNP:768169499
455 455 -, a dbSNP:753909971
461 461 a, g dbSNP:746582143
465 465 c, g, t dbSNP:771753477
480 480 g, t dbSNP:745514950
481 481 c, t dbSNP:778599826
483 483 a, t dbSNP:757040753
487 487 c, g dbSNP:749184660
490 490 a, g dbSNP:777706172
495 495 c, t dbSNP:756103311
496 496 c, g dbSNP:751753401
500 500 a, g dbSNP:766535284
506 506 c, t dbSNP:535476484
513 513 c, g dbSNP:750607594
514 514 a, g dbSNP:566450630
515 515 c, t dbSNP:762180740
516 516 a, t dbSNP:201888844
517 517 a, g dbSNP:764652387
521 521 c, t dbSNP:374175877
522 522 a, g dbSNP:761140317
528 528 c, t dbSNP:371097138
541 541 c, t dbSNP:771526677
545 545 -, c dbSNP:780299639
551 551 a, g dbSNP:745540793
554 554 c, g, t dbSNP:376630127
555 555 g, t dbSNP:749162539
556 556 g, t dbSNP:777702532
558 558 a, g dbSNP:539903027
559 559 c, t dbSNP:748075455
560 560 c, g dbSNP:547349748
575 575 g, t dbSNP:758510921
579 579 c, g dbSNP:750612755
583 583 c, t dbSNP:373469627
586 586 c, t dbSNP:201078416
604 604 g, t dbSNP:757418839
614 614 c, t dbSNP:201023208
615 615 c, t dbSNP:764421231
616 616 c, g dbSNP:761195604
619 619 a, t dbSNP:368970746
621 621 a, t dbSNP:767022079
625 625 g, t dbSNP:759025249
633 633 c, t dbSNP:773719228
635 635 a, g dbSNP:770679537
636 636 -, a dbSNP:756360957
637 637 g, t dbSNP:748857760
642 642 a, g dbSNP:375580763
645 645 -, agg dbSNP:750700198
645 645 a, t dbSNP:190812374
656 656 a, c dbSNP:769663941
657 657 c, t dbSNP:530916858
658 658 c, t dbSNP:781207627
661 661 a, g dbSNP:768465514
685 685 a, g dbSNP:763313333
688 688 a, g dbSNP:746029215
707 707 c, t dbSNP:775820623
709 709 a, g dbSNP:185891858
715 715 c, t dbSNP:541954840
716 716 g, t dbSNP:770344909
735 735 a, c dbSNP:749610366
756 756 c, g dbSNP:528516281
763 763 c, g dbSNP:559307674
771 771 c, g dbSNP:759936639
783 783 a, g dbSNP:540153905
787 787 c, t dbSNP:528100454
804 804 c, t dbSNP:577488389
833 833 a, g dbSNP:73568948
840 840 c, t dbSNP:544356178
853 853 a, g dbSNP:575339012
864 864 g, t dbSNP:555489004
870 870 -, a dbSNP:71338780
872 872 -, aag dbSNP:778914148
873 873 -, a dbSNP:71338779
874 874 g, t dbSNP:535182496
903 903 a, c dbSNP:563379703
904 904 c, t dbSNP:566488233
953 953 a, c dbSNP:552974949
956 956 c, g dbSNP:543389826
962 962 a, g dbSNP:540138385
964 964 c, t dbSNP:571115232
974 974 g, t dbSNP:182553559
1016 1016 a, g dbSNP:531181521
1032 1032 c, g dbSNP:771405313
1076 1076 -, c dbSNP:376254737
1084 1084 a, g dbSNP:575993357
1086 1086 c, t dbSNP:747645538
1100 1100 a, c dbSNP:568538750
1111 1111 c, t dbSNP:557652872
1140 1140 c, t dbSNP:544919803
1194 1194 c, t dbSNP:528532265
1203 1203 -, a dbSNP:150558316
1218 1218 a, g dbSNP:543505586
1234 1234 c, g dbSNP:369033022
1260 1260 a, g dbSNP:559344315
1268 1268 g, t dbSNP:545747717
1282 1282 c, t dbSNP:533069420
1290 1290 a, c dbSNP:564448491
1293 1293 c, t dbSNP:544179501
1307 1307 c, t dbSNP:575428147
1351 1351 a, c, g, t dbSNP:7259834
1360 1360 g, t dbSNP:191039825
1377 1377 c, g dbSNP:768132755
1419 1419 c, g dbSNP:572948185
1518 1518 a, g dbSNP:748979866
1584 1584 g, t dbSNP:779667115
1600 1600 c, t dbSNP:186186357
1601 1601 c, g dbSNP:539475374
1602 1602 -, g dbSNP:34997904
1643 1643 c, t dbSNP:755874224
1647 1647 c, t dbSNP:750164782
1685 1685 c, t dbSNP:115623295
1690 1690 -, tcct dbSNP:781432805
1694 1694 g, t dbSNP:557682873
1722 1722 a, g dbSNP:537335238
1780 1780 a, c dbSNP:150986093
1819 1819 c, t dbSNP:548446096
1843 1843 a, g dbSNP:534811674
1845 1845 a, g dbSNP:565799330
1883 1883 c, t dbSNP:142545664
1892 1892 a, c dbSNP:2270199
1903 1903 a, g dbSNP:751646789
1918 1918 a, g dbSNP:757166355
1925 1925 g, t dbSNP:563527167

Target ORF information:

RefSeq Version NM_001017989
Organism Homo sapiens (human)
Definition Homo sapiens optic atrophy 3 (autosomal recessive, with chorea and spastic paraplegia) (OPA3), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_001017989

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGTGGTGG GCGCGTTCCC TATGGCGAAG CTGCTATACT TGGGCATCCG GCAGGTCAGC 
AAGCCGCTTG CCAACCGTAT TAAGGAGGCC GCCCGCCGAA GCGAGTTCTT CAAGACCTAT
ATCTGCCTCC CGCCGGCTCA ACTGTACCAC TGGCTGGAGA TGCGGACCAA AATGCGCATC
ATGGGTTTCA ATGCCGCTGC CATCAAGCCG CTGAACGAGG GTGCAGCCGC CGAGCTGGGC
GCGGAGCTGC TGGGCGAGGG CATCATCTTC ATCACCGCCT GCAGCTGCCT GATGCTGGAG
TATTGGCGCC ACCAGTTGCA GCAGCGCCGC AAGGAAAAGG AGCGACGTGT TGCCAGGGAG
GCGCTGCGGG GCGAGGTGGG CCACTTGGGG CTGGCGCTCG AGGAGTTGCA GGCGCAGGTG
CAGGCGACGT CGACGCAGCT CGCCCTGGAG GAGCTGCGCG CTCAGCTGCA GGAGGTGCGA
GCCCACCTCT GCCTCCGAGA CCCGCCGCCT GCACCCCCAG TTGCGCCGGC GTCCGAGAAA
TAG

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu23186D
Sequence Information ORF Nucleotide Sequence (Length: 540bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product optic atrophy 3 protein isoform b
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB034102.1, BC005059.1, AC006261.1 and BU617706.1. On Oct 8, 2009 this sequence version replaced gi:156151425. Summary: The mouse ortholog of this protein co-purifies with the mitochondrial inner membrane. Mutations in this gene have been shown to result in 3-methylglutaconic aciduria type III and autosomal dominant optic atrophy and cataract. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]. Transcript Variant: This variant (2) represents the longer transcript but encodes the shorter isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 18614015, 12865426; reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AK025840.1, BC005059.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142670, SAMEA2154405 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
ATGGTGGTGG GCGCGTTCCC TATGGCGAAG CTGCTATACT TGGGCATCCG GCAGGTCAGC 
AAGCCGCTTG CCAACCGTAT TAAGGAGGCC GCCCGCCGAA GCGAGTTCTT CAAGACCTAT
ATCTGCCTCC CGCCGGCTCA ACTGTATCAC TGGGTGGAGA TGCGGACCAA GATGCGCATC
ATGGGCTTCC GGGGCACGGT CATCAAGCCG CTGAACGAGG AGGCGGCAGC TGAGCTGGGC
GCAGAGCTGC TGGGCGAAGC CACCATCTTC ATCGTGGGCG GCGGCTGCCT AGTGCTGGAG
TACTGGCGCC ACCAGGCGCA GCAGCGCCAC AAGGAGGAGG AGCAGCGTGC TGCCTGGAAC
GCGCTGCGGG ACGAGGTGGG CCACCTGGCG CTGGCGCTGG AAGCGCTGCA GGCGCAGGTG
CAGGCGGCGC CGCCACAGGG CGCCCTGGAG GAACTGCGCA CAGAGCTGCA AGAGGTGCGC
GCCCAGCTCT GCAATCCCGG CCGGTCCGCT TCCCACGCAG TGCCTGCGTC CAAGAAATAG
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_079412.1
CDS101..640
Misc Feature(1)119..481(+)
Exon (1)1..242
Gene:OPA3
Gene Synonym:
Exon (2)243..7873
Gene:OPA3
Gene Synonym:
Translation
Position Chain Variation Link
5 5 a, g, t dbSNP:566870092
10 10 c, t dbSNP:546632526
15 15 a, g dbSNP:73570932
16 16 g, t dbSNP:571483175
26 26 a, g dbSNP:45598532
30 30 c, g dbSNP:531556421
31 31 a, g dbSNP:569038733
35 35 a, g dbSNP:549109182
36 36 g, t dbSNP:528949164
42 42 a, t dbSNP:560188551
45 45 a, g dbSNP:539890732
52 52 a, g dbSNP:771334307
55 55 c, t dbSNP:749517349
61 61 a, c, g dbSNP:770257059
63 63 a, g dbSNP:45527139
64 64 a, g dbSNP:781509098
66 66 c, t dbSNP:754517296
71 71 a, g dbSNP:746562214
75 75 c, t dbSNP:200159837
77 77 c, g, t dbSNP:750059099
79 79 c, t dbSNP:201065312
84 84 a, c dbSNP:757005901
89 89 a, g dbSNP:201864058
90 90 a, c dbSNP:763882469
93 93 a, c dbSNP:370143397
94 94 c, g, t dbSNP:199590627
97 97 c, g dbSNP:774359394
112 112 c, t dbSNP:766535154
113 113 a, g dbSNP:749185315
115 115 c, g dbSNP:763315476
120 120 c, t dbSNP:373427314
121 121 c, t dbSNP:770310018
133 133 g, t dbSNP:148805518
134 134 c, g dbSNP:200742525
136 136 a, g dbSNP:769113318
139 139 c, t dbSNP:374843726
142 142 a, g dbSNP:779592921
143 143 c, g dbSNP:771522612
147 147 a, t dbSNP:745552499
148 148 a, c dbSNP:778472683
165 165 c, t dbSNP:757060614
194 194 a, c dbSNP:753632531
195 195 a, g dbSNP:777618441
212 212 a, g dbSNP:756044697
220 220 c, t dbSNP:752657560
221 221 a, g dbSNP:766584469
223 223 c, g dbSNP:763083098
229 229 c, t dbSNP:201438203
235 235 a, g dbSNP:553414785
247 247 c, t dbSNP:769477086
252 252 a, g dbSNP:747890876
262 262 a, g dbSNP:781119036
263 263 a, c dbSNP:748653611
264 264 c, g dbSNP:755008161
265 265 g, t dbSNP:746924502
267 267 c, t dbSNP:778885990
278 278 a, c dbSNP:757323736
290 290 c, t dbSNP:754011378
291 291 a, g dbSNP:764320249
294 294 g, t dbSNP:144382886
297 297 c, t dbSNP:753129998
301 301 c, t dbSNP:539986269
306 306 a, c dbSNP:760083523
308 308 c, t dbSNP:774790473
309 309 c, t dbSNP:143027616
310 310 a, g dbSNP:762454431
316 316 c, t dbSNP:772750356
324 324 c, t dbSNP:769541845
331 331 c, t dbSNP:3826860
340 340 c, t dbSNP:776619481
341 341 a, g dbSNP:186796646
348 348 a, t dbSNP:746977962
349 349 c, g dbSNP:199692583
352 352 a, g dbSNP:757297634
353 353 a, g dbSNP:749444775
368 368 -, ttc dbSNP:761741716
373 373 c, g, t dbSNP:369545089
374 374 a, g dbSNP:752893027
377 377 a, g dbSNP:80356524
383 383 g, t dbSNP:767889585
389 389 c, t dbSNP:755295420
391 391 a, g dbSNP:755689286
403 403 c, t dbSNP:752010426
406 406 c, g dbSNP:766847630
407 407 c, t dbSNP:763353585
413 413 c, g dbSNP:80356525
416 416 a, g dbSNP:750013718
417 417 c, t dbSNP:764633370
420 420 -, agcagcgccacaaggagg dbSNP:80356526
433 433 a, g dbSNP:761583193
436 436 a, g dbSNP:146349367
437 437 a, g dbSNP:142467378
451 451 c, t dbSNP:760489108
452 452 c, g, t dbSNP:772116334
454 454 c, t dbSNP:745763712
455 455 a, t dbSNP:375471439
459 459 a, g dbSNP:769813697
466 466 g, t dbSNP:748397260
469 469 a, g dbSNP:781320613
472 472 a, c dbSNP:755348694
479 479 a, g dbSNP:575871533
485 485 -, acc dbSNP:774167136
489 489 a, c dbSNP:780275755
495 495 -, a dbSNP:768199119
495 495 a, c dbSNP:758982194
500 500 -, cgctgg dbSNP:748815011
504 504 c, g dbSNP:372640359
505 505 g, t dbSNP:750848548
510 510 a, c dbSNP:372161100
512 512 a, g dbSNP:201574732
515 515 c, t dbSNP:28937899
524 524 a, g dbSNP:535683352
527 527 c, g dbSNP:567096759
528 528 c, t dbSNP:553598004
529 529 a, g dbSNP:745373208
531 531 c, g, t dbSNP:775398472
532 532 a, g dbSNP:45470595
542 542 g, t dbSNP:771739856
544 544 c, t dbSNP:759536853
550 550 a, g dbSNP:529981150
558 558 g, t dbSNP:539965360
570 570 -, aagaggtgc dbSNP:745544229
571 571 a, g dbSNP:570940748
576 576 c, t dbSNP:748168313
578 578 -, cttaact dbSNP:769486266
578 578 c, t dbSNP:137978109
580 580 c, t dbSNP:768932301
584 584 a, c dbSNP:747167154
587 587 c, t dbSNP:780492515
594 594 a, g dbSNP:558412882
602 602 c, g dbSNP:750946088
603 603 a, g dbSNP:779505447
607 607 c, t dbSNP:757829968
608 608 a, g dbSNP:374639297
617 617 a, g dbSNP:371832021
626 626 a, g dbSNP:755841329
628 628 a, g dbSNP:752240099
632 632 a, g dbSNP:767372876
634 634 -, gaaa dbSNP:778074016
637 637 a, g dbSNP:140105522
638 638 g, t dbSNP:780840710
640 640 a, g dbSNP:774281852
649 649 a, t dbSNP:201697957
650 650 g, t dbSNP:762873253
651 651 -, t dbSNP:35625615
658 658 c, t dbSNP:776730325
659 659 c, t dbSNP:768697570
667 667 a, g dbSNP:747289326
668 668 a, g dbSNP:367663395
671 671 a, c dbSNP:775509581
674 674 a, g dbSNP:772500238
676 676 c, t dbSNP:562456005
678 678 a, g dbSNP:746276271
679 679 c, t dbSNP:779419503
681 681 a, t dbSNP:757882898
683 683 c, t dbSNP:749792566
688 688 c, t dbSNP:146126868
700 700 c, t dbSNP:529172414
704 704 c, t dbSNP:559943742
720 720 g, t dbSNP:540023428
725 725 c, t dbSNP:532881285
749 749 a, g dbSNP:563811459
792 792 a, g dbSNP:3826861
866 866 c, g dbSNP:575138678
882 882 c, g dbSNP:144259826
884 884 c, t dbSNP:542553691
885 885 c, t dbSNP:139637265
912 912 a, g dbSNP:7246349
960 960 c, t dbSNP:541330507
966 966 -, gt dbSNP:571672143
1002 1002 c, t dbSNP:73942919
1038 1038 g, t dbSNP:183851663
1047 1047 c, t dbSNP:537060236
1062 1062 c, t dbSNP:116977551
1064 1064 c, g dbSNP:548266881
1128 1128 c, g dbSNP:780231275
1172 1172 -, g dbSNP:563314271
1180 1180 g, t dbSNP:149360920
1189 1189 a, g dbSNP:566396414
1217 1217 c, t dbSNP:112654759
1219 1219 c, g dbSNP:532917256
1235 1235 c, t dbSNP:539993852
1278 1278 a, t dbSNP:150575877
1281 1281 c, t dbSNP:550694350
1317 1317 c, t dbSNP:560749495
1333 1333 c, t dbSNP:530521545
1338 1338 a, g dbSNP:561748985
1353 1353 c, t dbSNP:143735864
1362 1362 a, g dbSNP:542591243
1407 1407 a, g dbSNP:138079196
1432 1432 a, g dbSNP:560058795
1443 1443 a, g dbSNP:773462602
1477 1477 c, t dbSNP:764268778
1478 1478 a, g dbSNP:546421510
1481 1481 c, t dbSNP:577472780
1534 1534 c, t dbSNP:575283920
1550 1550 a, g dbSNP:192099573
1579 1579 a, c dbSNP:537579700
1585 1585 c, t dbSNP:574822690
1596 1596 a, g dbSNP:554964579
1611 1611 -, a dbSNP:34303121
1626 1626 -, a dbSNP:377547137
1658 1658 a, g dbSNP:534682712
1666 1666 a, c dbSNP:566435083
1688 1688 a, g dbSNP:373689257
1692 1692 c, t dbSNP:539341907
1702 1702 c, t dbSNP:570363318
1710 1710 c, t dbSNP:146989248
1718 1718 c, t dbSNP:141205847
1731 1731 c, g dbSNP:557691359
1735 1735 a, c dbSNP:148228876
1749 1749 c, t dbSNP:769097772
1823 1823 a, g dbSNP:745391525
1824 1824 a, g dbSNP:548188163
1853 1853 c, t dbSNP:539298843
1854 1854 a, g dbSNP:368652706
1877 1877 c, t dbSNP:537481614
1878 1878 a, g dbSNP:187216413
1885 1885 c, t dbSNP:183541433
1886 1886 a, g dbSNP:191683025
1896 1896 a, g dbSNP:578235416
1910 1910 c, t dbSNP:543618194
1934 1934 -, a dbSNP:74313320
1935 1935 c, t dbSNP:574897391
1966 1966 c, g dbSNP:554718315
1970 1970 a, t dbSNP:746686870
1980 1980 c, t dbSNP:12104391
1996 1996 a, g dbSNP:777448675
2017 2017 a, g dbSNP:143022941
2027 2027 c, t dbSNP:73568986
2031 2031 a, g dbSNP:752520539
2087 2087 c, t dbSNP:527613456
2092 2092 c, t dbSNP:149045583
2183 2183 c, t dbSNP:570400661
2254 2254 c, t dbSNP:185624158
2269 2269 a, g dbSNP:754896580
2270 2270 c, t dbSNP:536820022
2282 2282 a, g dbSNP:144894771
2308 2308 a, c, g dbSNP:374292097
2319 2319 a, c, t dbSNP:528186706
2346 2346 a, g dbSNP:760778079
2349 2349 c, t dbSNP:766271983
2400 2400 c, t dbSNP:75401979
2401 2401 a, t dbSNP:141446737
2436 2436 a, g dbSNP:532870930
2476 2476 c, t dbSNP:74717111
2495 2495 c, t dbSNP:146599259
2503 2503 g, t dbSNP:769185702
2542 2542 a, g dbSNP:530192525
2577 2577 c, t dbSNP:181389751
2590 2590 a, g dbSNP:4404183
2597 2597 c, g dbSNP:775951781
2611 2611 c, t dbSNP:572183370
2620 2620 c, g dbSNP:558566166
2630 2630 g, t dbSNP:550844056
2648 2648 c, t dbSNP:144432336
2694 2694 c, g dbSNP:77272492
2741 2741 a, t dbSNP:139606810
2766 2766 c, g dbSNP:537261730
2783 2783 -, gt dbSNP:532263532
2824 2824 c, t dbSNP:746520443
2843 2843 a, g dbSNP:189820250
2856 2856 c, t dbSNP:554746723
2860 2860 a, g dbSNP:768236143
2876 2876 a, c dbSNP:771697355
2879 2879 a, g dbSNP:534738209
2882 2882 c, t dbSNP:565746392
2895 2895 g, t dbSNP:552223709
2960 2960 c, t dbSNP:185506809
3009 3009 a, c dbSNP:748798679
3028 3028 c, t dbSNP:778809359
3049 3049 a, g dbSNP:570337042
3052 3052 c, t dbSNP:550245504
3063 3063 c, t dbSNP:187831466
3083 3083 a, g dbSNP:530415520
3098 3098 a, c dbSNP:561347971
3109 3109 a, g dbSNP:115566341
3124 3124 c, t dbSNP:753867707
3125 3125 a, g dbSNP:8106584
3130 3130 c, t dbSNP:11671670
3131 3131 a, g dbSNP:375563198
3133 3133 a, g dbSNP:756206551
3158 3158 a, g dbSNP:565012397
3209 3209 c, t dbSNP:142346821
3223 3223 c, t dbSNP:180882043
3253 3253 a, g dbSNP:573757220
3260 3260 a, c dbSNP:528208456
3294 3294 a, g dbSNP:757365326
3296 3296 c, t dbSNP:377431772
3336 3336 c, g dbSNP:148705681
3354 3354 c, t dbSNP:781539538
3356 3356 c, t dbSNP:574653920
3407 3407 a, c dbSNP:11667724
3415 3415 a, g dbSNP:144240983
3499 3499 a, g dbSNP:534780094
3536 3536 g, t dbSNP:190488484
3593 3593 -, tag dbSNP:554461162
3678 3678 a, g dbSNP:73568982
3704 3704 c, g dbSNP:751946259
3715 3715 c, t dbSNP:538725909
3716 3716 a, g dbSNP:371025254
3719 3719 c, g dbSNP:377696221
3748 3748 a, c dbSNP:201040372
3750 3750 -, agc dbSNP:10650481
3751 3751 -, agc dbSNP:72528722
3752 3752 -, agc dbSNP:386389097
3755 3755 -, cag dbSNP:35934096
3756 3756 -, agc dbSNP:542311224
3757 3757 c, t dbSNP:569595277
3768 3768 c, g dbSNP:143702010
3802 3802 a, g dbSNP:139795580
3804 3804 c, t dbSNP:559474405
3806 3806 c, t dbSNP:577805330
3816 3816 c, t dbSNP:113095552
3817 3817 a, g dbSNP:530530814
3847 3847 a, g dbSNP:764494903
3852 3852 a, g dbSNP:79317386
3862 3862 c, g, t dbSNP:73568980
3918 3918 c, t dbSNP:770317628
3920 3920 a, g dbSNP:185631253
3929 3929 a, g dbSNP:147400347
3960 3960 g, t dbSNP:142831259
3990 3990 g, t dbSNP:181719786
3993 3993 -, tcttt dbSNP:373580645
3997 3997 a, t dbSNP:772710835
4000 4000 a, g dbSNP:545764728
4016 4016 a, g dbSNP:189068529
4027 4027 a, g dbSNP:3760844
4034 4034 a, g dbSNP:561119752
4047 4047 c, t dbSNP:541235547
4079 4079 a, c dbSNP:553328047
4082 4082 a, g dbSNP:138449967
4083 4083 a, g dbSNP:768641413
4102 4102 c, t dbSNP:558779723
4136 4136 c, g dbSNP:749084024
4171 4171 g, t dbSNP:112899014
4221 4221 c, t dbSNP:576070088
4231 4231 a, g dbSNP:541630766
4340 4340 a, g dbSNP:780154471
4350 4350 c, t dbSNP:183951390
4362 4362 a, g dbSNP:73568973
4375 4375 a, g dbSNP:750504205
4395 4395 a, g dbSNP:555830952
4398 4398 g, t dbSNP:757264789
4400 4400 c, t dbSNP:534214560
4454 4454 c, t dbSNP:191869562
4468 4468 c, g dbSNP:71352273
4499 4499 a, g dbSNP:551539059
4514 4514 a, g dbSNP:62109650
4518 4518 a, g dbSNP:79660166
4524 4524 a, g dbSNP:187028696
4531 4531 c, t dbSNP:752997579
4603 4603 a, c dbSNP:7247946
4610 4610 c, t dbSNP:529245346
4612 4612 c, t dbSNP:147972697
4619 4619 a, g dbSNP:546196266
4638 4638 a, g dbSNP:1142781
4654 4654 a, c dbSNP:200851409
4655 4655 a, c dbSNP:74409501
4662 4662 a, c dbSNP:541381652
4705 4705 c, t dbSNP:184285761
4714 4714 a, g dbSNP:762519965
4716 4716 c, t dbSNP:371821013
4724 4724 c, t dbSNP:538573848
4745 4745 c, t dbSNP:369558782
4771 4771 -, gccct dbSNP:3038838
4771 4771 -, gccct dbSNP:397714484
4773 4773 c, t dbSNP:200306337
4784 4784 -, ctgcc dbSNP:774875450
4787 4787 -, ccctg dbSNP:68079762
4791 4791 -, gacag dbSNP:199999897
4828 4828 a, g dbSNP:565107857
4830 4830 g, t dbSNP:192386255
4873 4873 a, g dbSNP:576081836
4881 4881 c, t dbSNP:375276027
4897 4897 a, c dbSNP:765665312
4901 4901 c, g dbSNP:760047356
4913 4913 g, t dbSNP:556179578
4958 4958 c, t dbSNP:4802261
4994 4994 g, t dbSNP:188949964
5012 5012 a, g dbSNP:573344031
5014 5014 g, t dbSNP:184015981
5029 5029 -, t dbSNP:543378137
5041 5041 a, g dbSNP:767102793
5057 5057 c, t dbSNP:190583213
5058 5058 a, g dbSNP:571592906
5067 5067 c, t dbSNP:558085353
5079 5079 c, t dbSNP:538388889
5082 5082 a, g dbSNP:768366106
5113 5113 c, t dbSNP:747411447
5264 5264 c, t dbSNP:749100531
5282 5282 a, g dbSNP:775318953
5312 5312 a, g dbSNP:11669246
5332 5332 -, taaa dbSNP:201924090
5333 5333 -, taaa dbSNP:58537694
5363 5363 -, ataa, ataaataa dbSNP:139897733
5403 5403 a, c dbSNP:549495140
5418 5418 c, t dbSNP:187663486
5434 5434 c, t dbSNP:114917731
5439 5439 c, t dbSNP:745868671
5479 5479 -, aaa dbSNP:10577501
5487 5487 a, t dbSNP:546999740
5489 5489 -, aaaa dbSNP:761709799
5489 5489 -, aaa dbSNP:761676585
5490 5490 -, aaa dbSNP:144752998
5497 5497 a, t dbSNP:4803833
5534 5534 c, t dbSNP:748204782
5588 5588 c, t dbSNP:565146816
5589 5589 a, g dbSNP:544881797
5594 5594 c, t dbSNP:146646433
5595 5595 a, g dbSNP:182678907
5615 5615 c, t dbSNP:567309052
5650 5650 -, c dbSNP:35934373
5653 5653 a, g dbSNP:780964248
5685 5685 c, t dbSNP:573380409
5686 5686 a, g dbSNP:553386926
5691 5691 c, g dbSNP:373641000
5730 5730 c, t dbSNP:539808989
5748 5748 c, g dbSNP:577920303
5757 5757 c, t dbSNP:371869405
5793 5793 -, c dbSNP:35111539
5821 5821 a, g dbSNP:558389168
5842 5842 a, g dbSNP:747076255
5868 5868 -, c dbSNP:770080585
5888 5888 a, g dbSNP:777979909
5902 5902 c, g dbSNP:538145979
5916 5916 a, c, t dbSNP:190072684
5917 5917 a, g dbSNP:535986790
5946 5946 g, t dbSNP:566934008
5948 5948 c, t dbSNP:367895706
5996 5996 c, t dbSNP:547036498
5997 5997 a, g dbSNP:549141863
6005 6005 c, t dbSNP:73568970
6044 6044 g, t dbSNP:571464354
6055 6055 c, t dbSNP:753091333
6073 6073 c, g dbSNP:186395735
6083 6083 a, g dbSNP:531507176
6092 6092 c, t dbSNP:530620363
6102 6102 c, g dbSNP:562732935
6147 6147 a, g dbSNP:116846670
6150 6150 a, g dbSNP:180756622
6163 6163 a, g dbSNP:57585727
6165 6165 c, t dbSNP:117230006
6167 6167 c, t dbSNP:371305726
6205 6205 c, t dbSNP:145816878
6210 6210 c, t dbSNP:766783593
6211 6211 a, g dbSNP:577258344
6237 6237 c, t dbSNP:560019290
6252 6252 c, t dbSNP:563921651
6286 6286 a, g dbSNP:763873981
6306 6306 g, t dbSNP:544916147
6307 6307 c, t dbSNP:575830485
6327 6327 c, t dbSNP:377645867
6329 6329 c, t dbSNP:536027564
6341 6341 a, t dbSNP:573374347
6347 6347 a, g dbSNP:553465270
6379 6379 c, t dbSNP:375835737
6380 6380 a, g dbSNP:377333207
6391 6391 a, g dbSNP:774987793
6392 6392 c, t dbSNP:769674807
6421 6421 a, g dbSNP:570872927
6423 6423 a, g dbSNP:550679720
6439 6439 -, a dbSNP:397971047
6449 6449 -, a dbSNP:58766088
6456 6456 -, a dbSNP:35751414
6514 6514 a, g dbSNP:745642913
6521 6521 c, t dbSNP:538175864
6522 6522 a, g dbSNP:569024074
6540 6540 c, g dbSNP:77077970
6541 6541 a, g dbSNP:529244222
6543 6543 g, t dbSNP:559906641
6545 6545 a, g dbSNP:116705343
6562 6562 c, t dbSNP:770795002
6563 6563 a, g dbSNP:149788703
6584 6584 a, g dbSNP:190885103
6607 6607 a, g dbSNP:543825688
6647 6647 c, g dbSNP:777789100
6651 6651 a, g dbSNP:574184134
6652 6652 a, g dbSNP:11083772
6679 6679 c, g dbSNP:543554901
6687 6687 c, t dbSNP:562194205
6712 6712 a, g dbSNP:576104602
6720 6720 a, c dbSNP:542454467
6741 6741 a, g dbSNP:187296782
6744 6744 -, aa dbSNP:142661638
6746 6746 g, t dbSNP:754093205
6765 6765 a, g dbSNP:780617440
6773 6773 a, g dbSNP:756650917
6848 6848 c, t dbSNP:139798464
6849 6849 a, g dbSNP:546349839
6860 6860 c, t dbSNP:577241345
6887 6887 -, c dbSNP:34101094
6915 6915 c, t dbSNP:74689727
6920 6920 a, g dbSNP:537023417
6929 6929 a, g dbSNP:182293743
6934 6934 a, c dbSNP:114655581
6936 6936 c, t dbSNP:564675598
7007 7007 c, t dbSNP:535358403
7034 7034 a, g dbSNP:151030695
7082 7082 a, g dbSNP:76825983
7116 7116 c, g dbSNP:533061696
7122 7122 a, g dbSNP:759385177
7124 7124 a, g dbSNP:570422920
7148 7148 a, g dbSNP:550550509
7159 7159 c, t dbSNP:372521871
7160 7160 a, g dbSNP:530383166
7167 7167 a, g dbSNP:561661081
7192 7192 g, t dbSNP:188922477
7193 7193 c, t dbSNP:542594258
7194 7194 a, g dbSNP:184177890
7196 7196 a, g dbSNP:560024517
7206 7206 a, g dbSNP:546386601
7210 7210 c, g dbSNP:773109015
7255 7255 a, g dbSNP:369198028
7258 7258 a, g dbSNP:557129054
7266 7266 a, g dbSNP:139757997
7297 7297 a, g dbSNP:147443761
7314 7314 c, t dbSNP:534603782
7328 7328 a, g dbSNP:181576269
7338 7338 g, t dbSNP:768694312
7343 7343 c, t dbSNP:535393059
7362 7362 a, c dbSNP:566392487
7369 7369 c, g dbSNP:552769108
7388 7388 c, t dbSNP:539207846
7390 7390 g, t dbSNP:570533596
7407 7407 a, g dbSNP:142898530
7415 7415 a, g dbSNP:375848972
7430 7430 c, t dbSNP:10422253
7442 7442 a, g dbSNP:530660050
7448 7448 a, g dbSNP:188413392
7466 7466 a, g dbSNP:548194990
7542 7542 a, g dbSNP:528812143
7569 7569 a, t dbSNP:751011663
7596 7596 a, g dbSNP:369362617
7608 7608 c, t dbSNP:184867350
7624 7624 a, g dbSNP:111523448
7632 7632 c, t dbSNP:546383446
7655 7655 a, g dbSNP:112948303
7658 7658 c, g dbSNP:557041940
7725 7725 a, g dbSNP:67373144
7733 7733 c, t dbSNP:772273946
7736 7736 -, attat dbSNP:565521231
7746 7746 a, g dbSNP:748352885
7754 7754 a, t dbSNP:187719027
7781 7781 a, g dbSNP:372546768
7784 7784 -, at dbSNP:779005079
7821 7821 a, g dbSNP:74253369

Target ORF information:

RefSeq Version NM_025136
Organism Homo sapiens (human)
Definition Homo sapiens optic atrophy 3 (autosomal recessive, with chorea and spastic paraplegia) (OPA3), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_025136

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGTGGTGG GCGCGTTCCC TATGGCGAAG CTGCTATACT TGGGCATCCG GCAGGTCAGC 
AAGCCGCTTG CCAACCGTAT TAAGGAGGCC GCCCGCCGAA GCGAGTTCTT CAAGACCTAT
ATCTGCCTCC CGCCGGCTCA ACTGTATCAC TGGGTGGAGA TGCGGACCAA GATGCGCATC
ATGGGCTTCC GGGGCACGGT CATCAAGCCG CTGAACGAGG AGGCGGCAGC TGAGCTGGGC
GCAGAGCTGC TGGGCGAAGC CACCATCTTC ATCGTGGGCG GCGGCTGCCT AGTGCTGGAG
TACTGGCGCC ACCAGGCGCA GCAGCGCCAC AAGGAGGAGG AGCAGCGTGC TGCCTGGAAC
GCGCTGCGGG ACGAGGTGGG CCACCTGGCG CTGGCGCTGG AAGCGCTGCA GGCGCAGGTG
CAGGCGGCGC CGCCACAGGG CGCCCTGGAG GAACTGCGCA CAGAGCTGCA AGAGGTGCGC
GCCCAGCTCT GCAATCCCGG CCGGTCCGCT TCCCACGCAG TGCCTGCGTC CAAGAAATAG

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.