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ADCC & CDC assays
ADCC & CDC assays

ADCC assay (SC1544) and CDC assay (SC1545) services

Antibody therapy has been proven to be highly powerful for cancer treatment. Two important mechanisms used by antibody drugs to kill targeted tumor cells are Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC), and Complement Dependent Cytotoxicity (CDC). GenScript is pleased to present to you both a PBMC-based ADCC assay and natural kill cell-based ADCC assay. The readout is endpoint-driven (target cell lysis). The CDC assay uses normal human serum as the source of complement. By implementing strict QC standards, GenScript can provide the assurance of the efficacy and potency profiles of your therapeutic antibodies.

To save your cost and increase the speed of the experiments, GenScript offers complimentary target cells from a tumor cell panel. More information can be found in the link: /tumor_cell_lines.html.


ADCC mechanism
ADCC Case Study<

Figure 1 Concentration-dependent study of Herceptin-mediated ADCC on SK-BR-3 cells by using PBMC as effector cells (human IgG as negative control)

ADCC Case Study<

Figure 2 Concentration-dependent study of Erbitux-mediated ADCC on A431 cells by using freshly isolated NK cells as effector cells (Rituxan as negative control)

ADCC Case Study<

Figure 3 Concentration-dependent study of Humira-mediated ADCC on CHO/tm TNF-α cells by using PBMC as effector cells (human IgG as negative control). CHO/tm TNF-α was a cell line stably expressed membrane bound TNF-α at which the proteolytic cleavage site was modified.

How does ADCC work?

Adapted from http://www.biotek.com/resources/articles/automated-non-adioactive-assay-methods-for-adcc-cdc-assays.htmrl

Case Study 1: Rituxan® elicited CDC lysis of two CD20-positive leukemia cell lines

Cost-effective Custom Polyclonal Antibodies Services
       Cost-effective Custom Polyclonal Antibodies Services

NHS: normal human serum

Key features of GenScript's ADCC & CDC assay services

Related in-vitro pharmacology services:

Service name Catalog number
CellPower™ Lentiviral-based stable cell line SC1394A, SC1394B
Tumor cell line profiling SC1508, SC1509
Protease profiling SC1396
GPCR profiling SC1402, SC1485
Kinase profiling SC1395

*All ADCC assays are performed in China.

Quotation and Ordering


 
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