Left: Integrity of saRNA from GenScript and competitor is detected by
Agilent 5200 capillary electrophoresis;
Middle: 100 ng of GS-saRNA-1, GS-saRNA-2 vector based eGFP saRNA
(Unmodified) or competitor’s eGFP saRNA were transfected into HEK293T cells using Lipofectamine
MessengerMAX (Thermo Fisher). 24 and 48h after transfection, eGFP expression is observed by fluorescence
microscopy and analyzed by a micro plate reader.
Right: L6 level in A549 cells transfected with different eGFP saRNA was
detected by ELISA.