One Stop Solutions for IVT RNA

First in the market to achieve Gene-to-mRNA in just 3 weeks to drive innovations with our cutting-edge mRNA, circular RNA, and self-amplifying RNA technologies, seamlessly integrated with LNP delivery systems.

RNAs produced at GenScript are optimized with our proprietary production platform, ensuring the quality and expression efficiency.

Why GenScript

First to achieve Gene-to-mRNA in 3 weeks, driving your innovations with advanced mRNA, circular RNA, and self-amplifying RNA technologies, integrated with LNP delivery systems

Accelerate Discovery

Industry-leading 3-week turnaround time from Gene-to-mRNA for global customers

  • Integrated capabilities from sequence design to RNA synthesis and LNP formulation in one solution
  • Delivered > 4187 quality mRNA orders globally with 93% on-time delivery 

Drive Innovation

Cutting-edge mRNA, circular RNA, and self-amplifying RNA technologies, seamlessly integrated with LNP delivery systems 

  • GenCap mRNA with high capping efficiency and UTR options
  • Cap2 mRNA for better expression at stressed condition
  • Circular RNA with enhanced circularization efficiency and high purity
  • saRNA  with reduced immunogenicity and high expression

Cultivate Collaboration

Proprietary & patented GenCap™ technologies open to licensing

Patented technologies covers:

  • Co-transcriptional Capping Method
  • Selection of UTRs for tissue specific targeting
  • Optimized PolyA Vector

Highlights

One-Stop Solution for ALL Your mRNA Needs

GenScript’s solution to IVT mRNA manufacturing streamlines your workflow from gene synthesis to mRNA production. Our IVT mRNAs are optimized with our proprietary production platform, ensuring the quality and expression efficiency of your mRNA.

Codon/UTR
Optimization
  • Codon optimization for protein expression offered every day for every gene
  • Codon optimization with >200 factors screened (patent)
Gene
Synthesis
  • 100 bp – 20 kb gene inserts
  • TAT as fast as 6 BDs to 14 BDs
  • Gene synthesis with Poly(A)-built-in vector
Cloning/Plasmid
Synthesis
  • Ready to use linearized plasmid DNA for IVT
  • Vectors encoded stable Poly(A) tracts with variance of only +/- 5-10 nt
mRNA
Synthesis
  • Customized RUO to preclinical grade mRNA service (Patent)
  • Advanced purification (Oligo dT, dsRNA removal) and variety of QC options
Lipid Nanoparticles
Delivery
  • Generic ionizable lipid formulation
  • Load 0.1- 30mg of mRNA, siRNA, miRNA,  DNA  and sgRNA delivery
  • LNP for gene editing

Comparison of different IVT RNA payload formats

Linear mRNA Self-Amplifying RNA Circular RNA
Structure Linear; Open 5’ and 3’ end
mRNA exonuclease degradation
Linear; Open 5’ and 3’ end
mRNA exonuclease degradation
Close loop; No free 5’ 3’ end
Eliminating mRNA exonuclease degradation
Ribosome recruiting 5’ Cap:
Initiates translation & stabilizes mRNA
5’ Cap:
Initiates translation & stabilizes mRNA
Internal Ribosome Entry Site (IRES) :
Initiates translation
ORF
  • Codon optimization for translation yield
  • Modified nucleosides, eg. M1Ψ
  • Insert < 20 kb
  • Codon optimization for translation yield
  • Modified nucleosides, eg. M5C
  • Insert < 6 kb
  • Codon optimization for translation yield
  • Insert < 4 kb
UTR Regulatory elements in the 5’ and 3’-UTR stabilize mRNA and increase translation initiation Replicon for further self-replication of the mRNA Include IRES + PIE + other elements
PolyA tail Stabilizes RNA molecule & Prevents degradation Stabilizes RNA molecule & Prevents degradation Not required
Protein Expression +++ Strong +++++ Strongest ++++ Stronger
Expression half-life +++ Shorter +++++ Longest ++++ Longer
Immunogenicity ++++ Low ++ High +++ Medium
Dosage requirement ++ High +++++ Lowest +++ Medium
Novelty +++ Traditional ++++ Novel +++++ Novel

Testimonials

Dr. Sang-Moo Kang Dr. Sang-Moo Kang

Institute for Biomedical Sciences at Georgia State University

GenScript mRNA has helped us make a rapid progress in developing novel vaccine projects. GenScript's service is faithful and timely provided, saving me lots of time to focus on research. This is why I have been using GenScript services for over 20 years.

Resources

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