Overview

Accelerate your research with GenScript's off-the-shelf catalog linear mRNA, circular RNA, self-amplifying RNA (saRNA), and Lipid Nanoparticle (LNP) products. Designed for faster delivery in 5 business days, large-scale orders, and seamless bundling with custom services, these solutions meet diverse application needs with exceptional quality. Backed by over 20 years of expertise in high-quality plasmid production, GenScript is your trusted partner in RNA innovation.

New Cap Analogue 2'OEt Cap1 AG-capped RNA Enhance Half Life In Vivo

The 2′-O-Ethyl-capped mRNA delivers the highest and most durable expression profile among all tested cap analogs, making it an optimal choice for in vivo applications requiring sustained protein output.

Products

T7 IVT kit with 2’OEt Cap1 AG

GenScript T7 IVT Kit with 2'OEt Cap1 AG is designed to produce in vivo-optimized mRNA with high RNA integrity, low dsRNA content, and >98% capping efficiency. The modified Cap1 structure supports enhanced mRNA expression, stability, and in vivo half-life, while optimized buffer conditions enable reliable synthesis of mRNA up to 10 kb without compromising yield or quality.

With 4.5X greater expression compared to natural cap and an approximately 10% longer half-life compared to market-available options, this kit provides a streamlined solution for discovery-scale IVT synthesis, construct screening, and downstream mRNA development.

 icon Capping efficiency

Capping efficiency
>98%

Cap1 incorporation via co-transcriptional capping

Half-life in vivo

Half-life in vivo
~2X natural Cap1

2’OEt Cap1 AG extends mRNA stability and translation efficiency

dsRNA byproducts

dsRNA byproducts
<0.1%

Engineered polymerase and pyrophosphatase minimize immunogenic dsRNA

Modified nucleotides

Modified nucleotides
m1Ψ

Reduces innate immune activation and increases mRNA stability

Case Study

Resources

mRNA Case Study Report

Case studies showing high expression efficiency of GenScript’s mRNA for expressing SB transposase, GFP, Luciferase, eSpCas9, and more.

Free Download

T7 IVT Kit with 2'OEt Cap1 AG

Co-transcriptional capping for high-yield, high-integrity

Free Download

Protocol GenScript T7 IVT Kit with 2’OEt Cap1 AG

Free Download

Case Study: Enhancing mRNA therapeutics with 2'OEt Cap1 AG

Free Download

FAQs

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