Overview

First in the market to achieve Gene-to-mRNA in just 3 weeks to drive innovations with our cutting-edge mRNA, circular RNA, and self-amplifying RNA technologies, seamlessly integrated with LNP delivery systems.

Why GenScript

Integrated Production Process icon

Fast Delivery for Speedy Discovery

Industry-leading 3-week turnaround time from Gene-to-mRNA for global customers

Cutting Edge Innovations

Innovations in mRNA, circular RNA, self-amplifying RNA, mRNA vector design, etc. with patented technologies for optimized RNA performance

Integrated Solutions

From gene to mRNA to LNP formulation

One-Stop Solution for ALL Your mRNA Needs

GenScript’s solution to IVT mRNA manufacturing streamlines your workflow from gene synthesis to mRNA production. Our IVT mRNAs are optimized with our proprietary production platform, ensuring the quality and expression efficiency of your mRNA.

Codon/UTR
Optimization
  • Codon optimization for protein expression offered every day for every gene
  • Codon optimization with >200 factors screened (patent)
Gene
Synthesis
  • 100 bp – 20 kb gene inserts
  • TAT as fast as 6 BDs to 14 BDs
  • Gene synthesis with Poly(A)-built-in vector
Cloning/Plasmid
Synthesis
  • Ready to use linearized plasmid DNA for IVT
  • Vectors encoded stable Poly(A) tracts with variance of only +/- 5-10 nt
mRNA
Synthesis
  • Customized RUO to preclinical grade mRNA service (Patent)
  • Advanced purification (Oligo dT, dsRNA removal) and variety of QC options
Lipid Nanoparticles
Delivery
  • Generic ionizable lipid formulation
  • Load 0.1- 30mg of mRNA, siRNA, miRNA,  DNA  and sgRNA delivery
  • LNP for gene editing

Size/Scale
Options

100 bp – 20 kb sequences

0.1mg to gram level

5' Cap Options

Cap0

Cap1 (Co-Capped or enzymatic)

Cap2 (Co-Capped)

Uncapped

Poly(A) Tail Options

100A Tail (recommended)

Custom tail ≤ 120A

No Tail

Modified NTP Options

N1-Me-Ψ

5moU

5meC &  Ψ

Cy5-UTP or Thio-CTP

Unmodified

5’-Biotin modified

N6-methyl-A

5-Iodo-C, 5-Iodo-U

Purification Options

LiCl precipitation

Oligo dT purification

dsRNA removal purification

Popular off-the-shelf mRNA catalogs available now!

Try our catalog mRNA expressing reporter genes, Cas protein, or SB-100 transposase, all delivered in just 5 days! Learn More >>

Quality Control and Specification

QC Item Test Method Specification RUO
Default
RUO
Upgrade
Preclinical
Default
Preclinical
Upgrade
Identification Appearance Visual Inspect Clear and free of foreign particles
RNA Length Capillary Electrophoresis Target ± 30%
RNA Length Agarose Gel Electrophoresis Expected size band detected
Poly A Length Enzyme digestion to LC-MS Target ± 5%
RNA Content UV Absorbance Target ± 5%
PH pH meter Target ± 0.5
Buffer Specification Client Spec N/A
Sequencing Sanger Sequencing >98%  Alignment
Sequencing NGS by Illumina Q20 Ratio > 95% Upon Request
Purity A 260/280 Ratio UV Spec 1.70 ~ 2.30
Capping Efficiency LC-MS ≥ 90%
Size based purity Capillary Electrophoresis ≥75%
Impurity Total protein residue Nano Orange Assay ≤ 1%
Plasmid DNA Residue qPCR ≤ 0.05%
dsRNA Slot-blot ≤ 0.1%
Aggregate quantification RNA Aggregation Assay <5%
Safety Endotoxin Semiquantitative < 10EU/mg
Endotoxin Quantitative < 10EU/mg
Bioburden Direct inoculation No Growth after
72 hrs

Note: Additional QC and characterizations are offered with an extra charge upon request.

Comparison of different IVT RNA payload formats

Linear mRNA Self-Amplifying RNA Circular RNA
Structure Linear; Open 5’ and 3’ end
mRNA exonuclease degradation
Linear; Open 5’ and 3’ end
mRNA exonuclease degradation
Close loop; No free 5’ 3’ end
Eliminating mRNA exonuclease degradation
Ribosome recruiting 5’ Cap:
Initiates translation & stabilizes mRNA
5’ Cap:
Initiates translation & stabilizes mRNA
Internal Ribosome Entry Site (IRES) :
Initiates translation
ORF
  • Codon optimization for translation yield
  • Modified nucleosides, eg. M1Ψ
  • Insert < 20 kb
  • Codon optimization for translation yield
  • Modified nucleosides, eg. M5C
  • Insert < 6 kb
  • Codon optimization for translation yield
  • Insert < 4 kb
UTR Regulatory elements in the 5’ and 3’-UTR stabilize mRNA and increase translation initiation Replicon for further self-replication of the mRNA Include IRES + PIE + other elements
PolyA tail Stabilizes RNA molecule & Prevents degradation Stabilizes RNA molecule & Prevents degradation Not required
Protein Expression +++ Strong +++++ Strongest ++++ Stronger
Expression half-life +++ Shorter +++++ Longest ++++ Longer
Immunogenicity ++++ Low ++ High +++ Medium
Dosage requirement ++ High +++++ Lowest +++ Medium
Novelty +++ Traditional ++++ Novel +++++ Novel

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Additional mRNA Offerings

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with GenScript IVT mRNA
Production Services

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