IVT mRNA Synthesis

GenScript One-stop Solutions

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Overview

Consistent, high-quality mRNAs are required to develop personalized mRNA therapeutics, such as patient-specific cancer vaccines or protein replacements for rare diseases.

In vitro transcribed (IVT) mRNA synthesis presents several key benefits, including simplified cell-free manufacturing, cell cycle-independent performance, and no risk of insertional mutagenesis.

GenScript offers custom IVT mRNA production via our expert RNA team based in Seattle, Washington. Our proprietary IVT RNA manufacturing workflow is both simple and versatile, with many customizable options.

IVT RNA Services

Size/Scale
Options

100 bp – 20 kb sequences

<0.2 mg to 200 mg

5' Cap Options

Cap0

Cap1 (Co-Capped or enzymatic)

Cap2 (Co-Capped)

Uncapped

Poly(A) Tail Options

100A Tail (recommended)

Custom tail ≤ 120A

No Tail

Modified NTP options

N1-Me-Ψ

5moU

5meC & Ψ

Unmodified

Contact an Expert Quote/Order Quote/Order

One-Stop Solution for ALL Your mRNA Needs

GenScript’s solution to IVT mRNA manufacturing streamlines your workflow from gene synthesis to mRNA production. Our IVT mRNAs are optimized with our proprietary production platform, ensuring the quality and expression efficiency of your mRNA.

Codon optimization for protein expression offered every day for every gene
Codon optimization with >200 factors screened (patent)

100 bp – 20 kb gene inserts
TAT as fast as 6 BDs to 14 BDs
Gene synthesis with Poly(A)-built-in vector

Ready to use linearized plasmid DNA for IVT
Vectors encoded stable Poly(A) tracts with variance of only +/- 5-10 nt

T7 polymerase, RNase inhibitors, DNase I, Proteinase K, enzymes commercially available
Mission to develop GenScript mRNA proprietary polymerases and capping enzymes

Customized RUO to preclinical grade mRNA service
Advanced purification (Oligo dT, dsRNA removal) and variety of QC options

Why GenScript

Integrated Production Process

Service covering from gene to mRNA synthesis

Proprietary mRNA Vector

For optimized IVT production

Uniform Poly(A) tail

Enhances expression efficiency

Purification & QC
Flexibility

Advanced purification (Oligo dT, dsRNA removal) and QC analysis available

Experienced Team with Optimized
Production Platform

Save your time & effort in designing and making mRNA

Customized / off-the-shelf mRNA

Variety of choices to fulfill your research needs

Testimonials

Dr. Sang-Moo Kang

Institute for Biomedical Sciences at Georgia State University

GenScript mRNA has helped us make a rapid progress in developing novel vaccine projects. GenScript service is faithful and timely provided, saving me lots of time to focus on research. This is why I have been using GenScript services for over 20 years.

Quality Control and Specification

QC	Item	Test Method	Specification
Identification	Appearance	Visual Inspect	Clear and free of foreign particles
	RNA Length	Capillary Electrophoresis	Target ± 30%
	RNA Length	Agarose Gel Electrophoresis	Expected size band detected
	Poly A Length	Enzyme digestion and CE	Target ± 30%
	RNA Content	UV Absorbance	Target ± 5%
	PH	pH meter	Target ± 0.5
	Buffer Specification	Client Spec	N/A
Purity	A 260/280 Ratio	UV Spec	1.70 ~ 2.30
	Capping Efficiency	LC-MS	≥ 90%
	Size based purity	Capillary Electrophoresis	≥75%
Impurity	Total protein residue	Nano Orange Assay	≤ 1%
	Plasmid DNA Residue	qPCR	≤ 0.05%
	dsRNA	Slot-blot	≤ 0.1%
Safety	Endotoxin	Semiquantitative	< 10EU/mg
	Endotoxin	Quantitative	< 10EU/mg
	Bioburden	Sdirect Inoculation	No Growth after 48 hrs