In vivo delivery efficiency of two different LNP formulations was compared by
delivering
Fluc mRNA (100% N1-methyl-pseU modified, GenScript) into Balb-C mice via IV injection at a
dose of 0.3mg/kg. The expression of Fluc mRNA was measured by whole-body bioluminescence
imaging.
In imaging analysis, Fluc-mRNA delivered by SM102-LNP showed higher expression efficiency
than MC3 after 8 and 48 hours. To evaluate the biodistribution profile of different
formulations, heart, liver, lung, spleen, kidney, and brain of the mice were collected and
imaged after 48 hours, SM102 -LNP showed stronger liver accumulation compared to MC3-LNP
after 48 hours. MC3-LNP showed stronger spleen accumulation after 48 hrs. Neither
formulation caused significant body weight loss during the experiment period for 3 days.