Library assembly is powered by our expertise in de novo gene synthesis and advanced semiconductor technology
Susan Butler
Rice University
I wanted to create mutant capsids for adeno-associated virus (AAV) vectors to improve targeting and transduction levels. GenScript was able to create a combinatorial mutant library that had 100% coverage of all expected variants before and after viral packaging, and excluded variants that contained amino acids that I did not want present at these sites. Having a library with only the variants that I wanted to test reduced my screening efforts and maximized my time in finding the optimal vector sequence.
Each bar represents a mutated position and each color represents the frequency of variants with a particular amino acid.
A combinatorial mutagenesis library for 4 sequential amino acid positions was created using either degenerate (NNK) codons or using our precise oligos. Specific amino acids were requested at each site.
Using our semiconductor-based oligos in library construction:
Eliminates the significant codon bias incurred with NNK degenerate oligos
Achieves user-defined amino acid distribution with equal representation of variants
Mutate several positions one at a time within a sequence located either consecutively or scattered throughout the protein sequence.
Each position is mutated to all 20 amino acids or specified codons;
Variants are delivered as small subpools , with each pool containing variants of a single amino acid position.
Target a consecutive string of amino acids within a defined region of the sequence or the whole protein sequence.
Each position is mutated to all 20 amino acids or specified codons;
Variants from all positions delivered as a single pool .
Mutate multiple positions simultaneously across specific target regions of a protein.
Only include desired codons;
User-defined amino acid mutations at each position with defined amino acid ratios;
All variants delivered as a single pool, with each variant containing multiple mutations.
Reducing Screen Burden in Antibody Engineering Through GenScript's Synthetic Precision Libraries
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How to make random mutagenesis less random
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Mutant libraries are delivered either as a mixed pool of variants (saturation scanning or combinatorial mutagenesis libraries) or as individual subpools (site saturation mutagenesis libraries), with each pool containing all possible variants for a single amino acid position.
We offer both next-generation sequencing (NGS) and Sanger sequencing to test library coverage and diversity.
Application Note: Reducing Screen Burden in Antibody Engineering Through GenScript's Synthetic Precision Libraries New Application Note: Reducing Screen Burden in Antibody Engineering Through GenScript's Synthetic Precision Libraries New
Feature Article in Nature: How to make random mutagenesis less random Feature Article in Nature: How to make random mutagenesis less random
Engineered antibodies: semi-rational design approach for faster optimization Engineered antibodies: semi-rational design approach for faster optimization
(Webinar) Rapid assembly of gene variant libraries to engineer proteins for user defined goals
(Webinar) Precision Mutant Library Helps In Protein Engineering New
A well-constructed mutant library can be a very useful tool for a variety of protein engineering projects, including:
Download and submit your mutant library design. Email the completed form to [email protected] or securely upload it at Precision Mutant Library.
Our technical account manager will contact you with a quote within 24 hours.
*For PCR product deliverables only. For more information about our rapid TAT, please contact us at [email protected]
