Application Note: Accelerating Antibody Engineering with DNA Mutant Libraries: A Two Rounds Strategy to Achieve 1000-Fold Affinity Boost

Accelerating Antibody Engineering with DNA Mutant Libraries: A Two Rounds Strategy to Achieve 1000-Fold Affinity Boost

Executive Summary: Through two rounds of optimization using GenScript’s DNA Mutant Library, significant improvements were achieved in the affinity maturation of a monoclonal antibody. Initially, a saturation scanning library was created with 63 amino acids and 19 non-wild type amino acids per position across 6 CDR regions. The top 5 candidates were selected for a second round of combinatorial optimization. The novel antibody from this mutant library showed a 1000-fold increase in binding affinity compared to the wildtype.

Highlights:

• Streamline Your Screening: Learn how DNA mutant libraries can minimize screening efforts in antibody engineering.
• Master Mutant Library Design: Uncover the secrets of effective library design, construction, and screening.
• Unlock Advanced Technology: Explore the advantages of our GenTitan chip-based DNA synthesis over traditional methods.