Figure 1:TRAC knockout in primary T cells using basic GMP Grade GenCRISPR™ Ultra Cas9 or GenCRISPR™ Ultra eSpCas9 with electroporation
The cells were transfected with GenCRISPR™ Ultra NLS-SpCas9-basic GMP (Cat. No. Z03623) or GenCRISPR™ Ultra eSpCas9-2NLS-basic GMP (Cat. No. Z03624), and sgRNA (synthesized by GenScript CRISPR Synthetic sgRNA Services) for human TRAC gene knockout by electroporation. After transfection and cell culture, the TRAC knock-out efficiency was analyzed by FACS. This data indicated that both basic GMP Grade GenCRISPR™ Ultra Cas9, and GenCRISPR™ Ultra eSpCas9 enable consistent and high gene knockout efficiency in primary T cells.
Note:
No Cargo: No Cas9 protein added (Negative control)
No EP: No electroporation for cell transfection (Negative control)
D3: KO efficiency evaluated after day 3
D6: KO efficiency evaluated after day 6
Cell Type: | Primary T cells |
Transfection Method: | Lonza 4D-Nucleofector® Transfection System |
Target Gene: | TRAC |
Cell amount: | 1.0 x 106 cells |
Cas9:sgRNA ratio: | 1:2 (25 pmol : 50 pmol) |
Cas9 Nucleases: | Z03623 GenCRISPR™ Ultra NLS-Cas9-basic GMP Z03624 GenCRISPR™ Ultra eSpCas9-2NLS-basic GMP |