Overview

The simplicity and versatility of CRISPR gene editing technology contribute to its utilization in various downstream applications, including gene and cell therapy, cell line engineering, animal model generation, and in vitro diagnostics. Through our partnership with Feng Zhang's laboratory at the Broad Institute of MIT and Harvard*, GenScript offers validated GMP Grade and RUO Grade Cas9, Cas12a and Cas13a nuclease products, sgRNA synthesis and HDR donor template synthesis services, and resources to help with leveraging CRISPR gene editing for basic research and clinical manufacturing.

Highlights

RNP-mediated CRISPR Gene Editing

  • Non-viral delivery, no risk of gene integration
  • Fast editing action and degradation
  • Low cytotoxicity and immunogenicity

Consistent high knockout and knock-in efficiency

  • In tested cell lines and primary T cells
  • At different electroporation scales
  • Using all grades of Ultra Cas9 Nucleases

High Lot-to-lot Consistency

  • In in vitro cleavage assay
  • In gene editing applications

Selection Guide

Applications Recommendations Cat. No. Product Names
Gene KO/KI with high editing efficiency Ultra SpCas9 (wt) Z03621 GenCRISPR™ Ultra NLS-Cas9-Research
Z03623 GenCRISPR™ Ultra NLS-Cas9- basic GMP
Z03623-GMP GenCRISPR™ Ultra NLS-Cas9-GMP
Gene KO/KI with low off-target effects Ultra eSpCas9 (mt) Z03622 GenCRISPR™ Ultra eSpCas9-2NLS-Research
Z03624 GenCRISPR™ Ultra eSpCas9-2NLS-basic GMP
Z03624-GMP GenCRISPR™ Ultra eSpCas9-2NLS-GMP
Gene KI with high KI efficiency SpCas9 with optimized NLS Z03701 GenCRISPR™ Cas9 v1.1
Z03702 GenCRISPR™ Cas9 v1.2
Gene KO/KI following with flow cytometry or other fluorescent assays SpCas9-eGFP fusion protein Z03393 GenCrispr NLS-Cas9-EGFP Nuclease
Z03467
Gene KO/KI with lower MW Cas nucleases AsCas12a Z03502 GenCRISPR™ Cas12a (Cpf1) Nuclease
LbCas12a Z03753 GenCRISPR™ LbCas12a Nuclease
SaCas9 Z03699 GenCRISPR™ SaCas9 2NLS Nuclease
in vitro Diagnostics LwaCas13a Z03486 GenCRISPR™ Cas13a (C2c2) Nuclease
LbuCas13a Z03742 GenCRISPR™ LbuCas13a Nuclease
AsCas12a Z03502 GenCRISPR™ Cas12a (Cpf1) Nuclease
LbCas12a Z03753 GenCRISPR™ LbCas12a Nuclease

Product List

SpCas9 Nuclease (wild-type)

Recombinant wild-type S. pyogenes Cas9 nucleases, purified from E. coli expression and contains various nuclear localization sequences (NLSs).

Cat. No. Product Name Quantity Price Order
Z03385
GenCrispr Cas9-C-NLS Nuclease
$146.00
 
Z03386
GenCrispr Cas9 Nuclease
$35.00
 
Z03388
GenCrispr Cas9-N-NLS Nuclease
$146.00
 
Z03389
GenCrispr NLS-Cas9-NLS Nuclease
$146.00
 
Z03468
GenCrispr Cas9-N-NLS Nuclease
$188.00
 
Z03469
GenCrispr NLS-Cas9-NLS Nuclease
$188.00
 
Z03621
GenCRISPR™ Ultra NLS-Cas9-Research
$695.00
 
Z03623
GenCRISPR™ Ultra NLS-Cas9-basic GMP
$209.00
 
Z03623-GMP
GenCRISPR™ Ultra NLS-Cas9-GMP
$6900.00
 
Z03701
GenCRISPR™ Cas9 v1.1
$179.00
 
Z03702
GenCRISPR™ Cas9 v1.2
$179.00
 

eSpCas9 Nuclease (mutant)

Recombinant mutant S. pyogenes Cas9 nucleases, purified from E. coli expression and contain various nuclear localization sequences (NLSs).

Cat. No. Product Name Quantity Price Order
Z03470
GenCrispr eSpCas9-N-NLS Nuclease
$240.00
 
Z03622
GenCRISPR™ Ultra eSpCas9-2NLS-Research
$765.00
 
Z03624
GenCRISPR™ Ultra eSpCas9-2NLS-basic GMP
$209.00
 
Z03624-GMP
GenCRISPR™ Ultra eSpCas9-2NLS-GMP
$6900.00
 
Z03692
GenCRISPR™ Ultra eSpCas9-N-NLS Research, tag-free
$765.00
 

Cas9-eGFP Nuclease

Recombinant S. pyogenes Cas9 nuclease and eGFP fusion protein, purified from E. coli expression, and contains nuclear localization sequence (NLS) and C-terminal 6xHis tag. 

Cat. No. Product Name Quantity Price Order
Z03393
GenCrispr NLS-Cas9-EGFP Nuclease
$188.00
 
Z03467
GenCrispr NLS-Cas9-EGFP Nuclease
$241.00
 

SaCas9 Nuclease

Recombinant S. aureus Cas9 nuclease, purified from E. coli expression, and contains nuclear localization signals (NLSs) and no tag. 

Cat. No. Product Name Quantity Price Order
Z03699
GenCRISPR™ SaCas9 2NLS Nuclease
$188.00
 

Cas9 Nickase D10A

Recombinant S. pyogenes Cas9 Nickase D10A, purified from an E. coli strain expressing the protein, and contains nuclear localization sequences (NLSs).

Cat. No. Product Name Quantity Price Order
Z03390
GenCrispr NLS-Cas9-D10A Nickase
$39.00
 

Cas12a Nuclease

Recombinant Acidaminococcus sp. (strain BV3L6) Cas12a (cpf1) nuclease or Lachnospiraceae bacterium ND2006 Cas12a nuclease, purified from E. coli expression, and contains nuclear localization sequences (NLSs).

Cat. No. Product Name Quantity Price Order
Z03502
GenCRISPR™ Cas12a (Cpf1) Nuclease
$105.00
 
Z03753
GenCRISPR™ LbCas12a Nuclease
$169.00
 

Cas13a Nuclease

Recombinant Leptotrichia wadei Cas13a nuclease or Leptotrichia buccalis Cas13a nuclease, purified from E. coli expression.

Cat. No. Product Name Quantity Price Order
Z03486
GenCRISPR™ Cas13a (C2c2) Nuclease
$189.00
 
Z03742
GenCRISPR™ LbuCas13a Nuclease
$189.00
 

Gene Editing Kits

Cat. No. Product Name Quantity Price Order
L00688
GenCrispr Mutation Detection Kit
$180.00 25 reactions
 
L00689
GenCrispr sgRNA Screening Kit
$166.00 30 reactions
 
L00694
GenCrispr sgRNA Synthesis Kit
$379.00 20 reactions
 
Z03396
GenCrispr T7 Endonuclease I
$55.00
 

Cas Nuclease Antibodies

Cat. No. Product Name Quantity Price Order
A01885
GenCrispr Cas9 Antibody, pAb, Rabbit
$79.00
 
A01935
GenCRISPR™ SpCas9 Antibody (4A1), mAb, Mouse
$99.00 40 μg
 
A01936
GenCRISPR™ SpCas9 Antibody (14B6), mAb, Mouse
$99.00 40 μg
 
A01951
GenCRISPR™ SaCas9 Antibody (11C12), mAb, Mouse
$99.00 40 μg
 
A01952
GenCRISPR™ SaCas9 Antibody (26H10), mAb, Mouse
$99.00 40 μg
 

Case Study

Resources

CRISPR RNP User Manual
CRISPR RNP User Manual

A guide on how to use GenScript's RNP products for targeted genome editing

Free Download
 CRISPR RNP Handbook
CRISPR RNP Handbook

Enabling RNP-mediated CRISPR gene editing and transforming life science research

Free Download
Cas9 and sgRNA Protocol
Cas9 and sgRNA Protocol

Simple protocol for CRISPR gene editing using GenCRISPR™ Cas9 nuclease

Free Download
GenCRISPR gRNA Design Tool
GenCRISPR gRNA Design Tool

Interactive CRISPR guide RNA design tool

Learn More
GenCRISPR Gene Editing Solutions
GenCRISPR™ Gene Editing Solutions

All solutions you need for a successful CRISPR gene editing project

Free Download

Related Products

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