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CenCrispr Cas9 enzymes

GenCrispr Cas9 Enzymes

DNA-free gene editing can be achieved by using purified Cas9 enzymes with gRNA and transfecting them directly into your cells or protoplasts of interest. GenCrispr Cas9 nuclease is the recombinant Streptococcus pyogenes Cas9 protein purified from E. coli and can be used for genome editing by inducing site-specific double stranded breaks in double stranded DNA. Cas9 protein forms a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of the CRISPR/Cas9 system. The RNP complex recognizes the target site by matching gRNA with the genomic DNA sequence and leads to DNA breaks within 3 bases from the NGG PAM (Protospacer Adjacent Motif). Presence of a nuclear localization signal (NLS) fusion with Cas9 enzyme ensures nuclear localization.

Enzyme activity validation guarantee

The GenCrispr Gene editing technology has been licensed-in from the Broad Institute. The activity of all GenCrispr Cas9 nucleases has been validated by in-vitro or in-vivo assays. The bioactivity data for all enzymes is included in the protocol document.

Key applications

Applications Cas9 nuclease
Nuclear localization and in vivo gene-editing Double Strand Break
  • GenCrispr Cas9-C-NLS nuclease
  • GenCrispr Cas9-N-NLS nuclease
  • GenCrispr NLS-Cas9-NLS nuclease
Single Strand Break
  • GenCrispr NLS-D10A-Cas9 Nickase
FACS/Fluorescence
  • GenCrispr NLS-Cas9-EGFP nuclease
In vitro target DNA cleavage GenCrispr Cas9 nuclease

Cat. No.
Product Name
Size
Price
Quantity
Z03386-50 GenCrispr Cas9 Nuclease
50 μg (5×10μg)(0.2mg/ml)
$105.00
Z03386-10 GenCrispr Cas9 Nuclease
10 μg (0.2mg/ml)
$33.00
Z03385-50 GenCrispr Cas9-C-NLS Nuclease
50 μg (1mg/ml)
$139.00
Z03388-50 GenCrispr Cas9-N-NLS Nuclease
50 µg (1mg/ml)
$139.00
Z03388-100 GenCrispr Cas9-N-NLS Nuclease
100 µg (4mg/ml)
$179.00
Z03389-50 GenCrispr NLS-Cas9-NLS Nuclease
50 µg (1mg/ml)
$139.00
Z03389-100 GenCrispr NLS-Cas9-NLS Nuclease
100 µg (4mg/ml)
$179.00
Z03390-50 GenCrispr NLS-Cas9-D10A Nickase
50 µg (1mg/ml)
$219.00
Z03390-100 GenCrispr NLS-Cas9-D10A Nickase
100 µg (4mg/ml)
$319.00
Z03390-10 GenCrispr NLS-Cas9-D10A Nickase
10 µg (1mg/ml)
$99.00
Z03393-50 GenCrispr NLS-Cas9-EGFP Nuclease
50 µg (1mg/ml)
$179.00
Z03393-100 GenCrispr NLS-Cas9-EGFP Nuclease
100 µg (3mg/ml)
$229.00
 

Cat. No.
Product Name
Size
Price
Quantity
L00688-25 GenCrispr Mutation Detection Kit
25 reactions
$180.00
L00688-100 GenCrispr Mutation Detection Kit
100 reactions
$400.00
L00689-30 GenCrispr sgRNA Screening Kit
30 reactions
$166.00
L00689-100 GenCrispr sgRNA Screening Kit
100 reactions
$400.00
L00690-25 High-Efficiency gRNA-Cas9-GFP Plasmid (linear) Assembly Kit
25 reactions
$400.00
L00690-10 High-Efficiency gRNA-Cas9-GFP Plasmid (linear) Assembly Kit
10 reactions
$200.00
L00691-25 High-Efficiency gRNA-Cas9-Puro Plasmid (linear) Assembly Kit
25 reactions
$400.00
L00691-10 High-Efficiency gRNA-Cas9-Puro Plasmid (linear) Assembly Kit
10 reactions
$200.00
L00692-25 High-Efficiency gRNA-Cas9-GFP Plasmid Assembly Kit
25 reactions
$350.00
L00692-10 High-Efficiency gRNA-Cas9-GFP Plasmid Assembly Kit
10 reactions
$160.00
L00693-25 High-Efficiency gRNA-Cas9-Puro Plasmid Assembly Kit
25 reactions
$350.00
L00693-10 High-Efficiency gRNA-Cas9-Puro Plasmid Assembly Kit
10 reactions
$160.00
L00694-50 GenCrispr sgRNA Synthesis Kit
50 reactions
$900.00
L00694-20 GenCrispr sgRNA Synthesis Kit
20 reactions
$379.00
 

How to Order CRISPR/Cas9 products from GenScript

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