Overview

GenScript licenses CRISPR technology from the Broad Institute of MIT and Harvard. Our offerings include the latest CRISPR plasmids and databases developed by the CRISPR pioneering Feng Zhang laboratory. Broad Institute-validated plasmids are a well-tested platform for expressing CRISPR/Cas9, and avoid instability issues in RNA-based platforms.

Service Details

GenScript CRISPR Plasmid Repository

GenScript maintains a collection of more than 20,000 lentiCRISPRv2 plasmids containing guide RNA (gRNA) sequences pre-validated by the Broad Institute. Plasmids can be searched by gene name, symbol or ID on our gRNA Database.

Product Vector Selection Pricing  
GenCRISPR™ Plasmid Collection Lentiviral Amp, Puro $99 Broad gRNA Database

Enhanced CRISPR/SpCas9 PlasmidsNew!

Enhanced specificity SpCas9 (eSpCas9), also referred to as SpCas9 (K848A/K1003A/R1060A), is structurally engineered for improved target specificity by researchers at the Feng Zhang Laboratory at the Broad Institute (Slaymaker et al. 2016).  eSpCas9 is able to reduce off-target effects by over 10-fold, while maintaining robust on-target genome editing efficiency.

Cas9 genome editing is dependent on the separation of DNA double strands. Mismatches between sgRNA and untargeted DNA sequences can cause unspecific binding and cleavage. To improve genome editing specificity, SpCas9 with mutations K848A, K1003A, and R1060A was developed. Neutralization of these positively charged residues within the non-target strand groove of SpCas9 weakened non-target binding and encouraged on-target binding which requires more stringent Watson-Crick base pairing.

Product Vector Selection Pricing
eSpCas9 Plasmids Plasmid
Lentiviral
Puro or GFP $199 Order

SpCas9 Plasmids

Cas9 endonuclease is the research standard for gene editing. When combined with single guide RNA (sgRNA) sequences, these enzymes create site-specific double strand breaks (DSBs) in the genome.

SpCas9/sgRNAs can be mutually expressed in all-in-one vectors or separately expressed in dual vectors.

The optimal on-target SpCas9 PAM sequence is NGG.

SpCas9 also contains on-target affinity for NGA sequences.

Product Vector Selection Pricing  
SpCas9 Plasmids Plasmid
Lentiviral
AAV
Amp
Amp, Puro
Amp, Neo
Amp, GFP
$199 Order

SpCas9 Nickase Plasmids

SpCas9 nickase (Cas9n D10A) contains a mutation allowing the endonuclease to create single-strand nicks, as opposed to DSBs. Pairing two opposite facing gRNA sequences with SpCas9 nickase is an efficient method of gene editing that prevents unwanted indels from forming.

Product Vector Selection Pricing  
SpCas9 Nickase Plasmids Plasmid
Lentiviral
Amp
Amp, Puro
Amp, GFP
$199 Order

SaCas9 Plasmids

The Staphylococcus aureus Cas9 orthologue (SaCas9) is the preferred endonuclease for adeno-associated virus (AAV) applications. SaCas9 is approximately 1 kb shorter than SpCas9, and offers additional flexibility around AAV packaging constraints. The lower immunogenicity of AAV vectors makes SaCas9 well-suited for in vivo editing applications and therapeutics.

The optimal on-target SaCas9 PAM sequence is NNGRRT.

SaCas9 also contains significant on-target affinity for NNGRRN.

Product Vector Selection Pricing  
SaCas9 Plasmids AVV Amp $199 Order

Transcription Activation (SAM) Plasmids

The CRISPR/Cas9 Synergistic Activation Mediator (SAM) system has been engineered to enable transcriptional activation of downstream targets. The SAM system utilizes three different activators, VP64, P65, and HSF1, which are assembled onto a catalytically dead Cas9 (dCas9) complex to drive transcription.

The SAM complex is comprised of three components: a gRNA incorporating two MS2 RNA aptamers, a catalytically inactive dCas9-VP64 fusion protein, and a MS2-P65-HSF1 activator fusion protein.

The SAM system is capable of activation of both coding and non-coding genetic elements.

To search for Broad Institute pre-validated SAM gRNA sequences, visit our guide RNA Database.

Product Vector Selection Pricing  
SAM gRNA Plasmids Plasmid
Lentiviral
Amp
Amp, Zeo
$199 Order
SAM dCas9-VP64 Plasmids Lentiviral Amp, Blast
Amp, GFP
$50 Order
SAM MS2-P65-HSF1 Plasmids Lentiviral Amp, GFP
Amp, Hygro
$50 Order

Broad Institute Plasmid Collection

Broad Institute Plasmids are generated by the Broad Institute of Harvard and MIT. These plasmids contain a 17bp-1.8kb expressible linker in lieu of a customized sgRNA sequence, which can be modified by your laboratory.

Product Vector Selection Pricing  
eSpCas9 and SpCas9 Broad Plasmid Collection Plasmid
Lentiviral
Amp, Puro
Amp, GFP
$99 Order
SpCas9 Nickase
Broad Plasmid Collection
Plasmid Amp
Amp, Puro
Amp, GFP
$99 Order
SaCas9
Broad Plasmid Collection
AAV Amp $99 Order

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