RNP-mediated CRISPR gene editing is becoming a new standard in gene and cell therapy. GenScript has developed hundreds of Cas9, Cas12a, Cas13a, MAD7, ABE, CBE, and prime editors for numerous gene editing companies. Plus, we have licensed a collection of validated GMP and RUO Grade catalog products from the Broad Institute.
Guaranteed delivery
Yield and purity guaranteed
Industrial-leading Speed
As Fast as 2 weeks
Activity Assay Development
Established protocol with inhouse sgRNA & DNA substrate
Scale-up Capacity
Track record of GMP projects
| Cas nuclease variants | Base editor/Prime editor | |
|---|---|---|
| Amount | Guaranteed delivery of 500ug, 1mg, 5mg, 10mg, or 20mg | Guaranteed delivery of 500ug, 1mg, or 5mg |
| Tag | His tag | |
| QC Standard |
|
|
| Timeline | 2-4 weeks | |
Cas9 nuclease in vitro cleavage test
GenScript provides comprehensive solution for in vitro activity assays
Test result shows that the custom Cas9 produced by GenScript exhibited robust cleavage activity on target dsDNA
Red arrow: Full-length dsDNA
substrate
Green arrow: Fragment-1 dsDNA
Blue arrow: Fragment-2 dsDNA
Customer Requirement: Deliver the Cas12a mutant and perform an activity assay
Delivery of the high purity Cas12a mutant
In vitro cleavage test result shows that the Cas12a mutant exhibits better cleavage activity than the wild type Cas12a
QC report
| Concentration by Bradford | Endotoxin | Purity by SDS-PAGE | Purity by SEC-HPLC | DNase detection | Amount |
|---|---|---|---|---|---|
| 4.93mg/mL | 0.648EU/mg | 90% | 94% | No detected | ~5mg |
In vitro cleavage test
Red arrow: Full-length
dsDNA substrate
Green arrow: Fragment-1 dsDNA
Blue arrow: Fragment-2 dsDNA
Production of numerous base editors and prime editors
Base editors and prime editors are gaining more attention in gene editing era. However, their complex structures, including multiple protein and RNA components, make them challenging to express and purify.
At GenScript, we have successfully produced numerous base editors and prime editors both in high purity and decent yield.
Adenine base editor (ABE)
Cytosine base editor (CBE)
Prime editor (PE)
| Type | ABE (TadA_nCas9) | CBE (APOBEC_nCas9) | PE (Cas9_RT) |
|---|---|---|---|
| Purification procedure |
|
|
|
| Purity | >90%, by SDS-PAGE under reducing conditions. | >90%, by SDS-PAGE under reducing conditions. | >90%, by SDS-PAGE under reducing conditions. |
| Yield | 1.6mg/L | 22.03mg/L | 12.3mg/L |
Customer Requirement:
Successfully delivered in 9 months
Test result shows that the Cas variant can induced deletion of target genome in all 3 host cells efficiently.
| Test items | Specifications | Results |
|---|---|---|
| Appearance | Clear, colorless liquid | PASS |
| Concentration | 10 ± 1 mg/mL | 9.5 mg/mL |
| Purity | >95% | 100% |
| Sterility | Negative | PASS |
| Endotoxin | < 0.5 EU/mg | < 0.25 EU/mg |
| Host Cell Protein | <50 ng/mg | 3.9 ng/mg |
| Residual DNase | < LOD | PASS |
| Residual RNase | < LOD | PASS |
| Host Cell DNA | <1 ng/mg | < 0.01 ng/mg |
