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Interactive CRISPR gRNA design tool

sgRNA designed on-demand for any DNA sequence you input

algorithm developed and validated by Feng Zhang lab, Broad Institute

CRISPR sgRNA Design Tool

GenScript is proud to offer free online access to our gRNA sequence design tool, developed by the Broad Institute of Harvard and MIT. Our gRNA design tool will identify single guide RNAs for use with wild-type S. pyogenes Cas9 for any DNA sequence you input. Start your gRNA design project by entering a sequence up to 250bp in length below.

Submit a single sequence for CRISPR design and analysis.

Sequence Name

Target Genome


We’ll launch the batch mode for gRNA design tool soon. At the mean time, please send your sequences to support@genscript.com. We’ll send you designed gRNA sequences via Email.

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