Home » Broad gRNA Design Tool for Any Sequence

CRISPR Resources

GenScript is proud to offer free online access to our gRNA sequence design tool, developed by the Broad Institute of Harvard and MIT. Our gRNA design tool will identify single guide RNAs for use with wild-type S. pyogenes Cas9 for any DNA sequence you input. Start your gRNA design project by entering a sequence up to 250bp in length below.

  • Single Sequence

  • Batch Mode

Submit a single sequence for CRISPR design and analysis.

GenCRISPR Services

Synthetic CRISPR sgRNA
Synthetic CRISPR sgRNA
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HDR Knock-in Templates
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Cas Nucleases
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