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pegRNA design tool

  • Enter Gene Symbol

  • Enter Sequence

Exon (negative direction) Exon (positive direction) Deletion Insertion sgRNA Translation Previous translation
Sequence Flip Tool

Please note the GFP sequence insertion does not contain a stop codon. A stop codon like TAA should be added if the insertion is at the end of the protein translated.

Output for Gene Symbol (Transcript ID )

Download Result

Input Sequence

Output Sequence

Please enter 17-23nt guide RNA sequence.

Select editing position:

  • The green label represents the targeted editing site.
  • When the mouse is placed on both ends of the green label and changes into a cross shape, press and hold the left mouse button to adjust the position on the left and right ends of the green label, and select a suitable editing position.

Go through sequence map:

  • By placing the mouse cursor on the sequence map and scrolling the mouse wheel, you can zoom in on the sequence map to display each base and amino acid.
  • Press and hold the left mouse button to move the sequence map freely to the left or right.
The ranking is based on “distance to mutation” of different sgRNA, the smaller this parameter is, the higher the ranking is. If the “distance to mutation” is the same, the higher the “on target score” of sgRNA is, the higher the ranking is. Please select design according to your most important parameter.

The total insertion length is within 100 bp.

Please enter the information according to your request:

  • Point mutation: select the codon or base need to be mutated in the sequence map by move the green label and deleted it directly. Then enter the new codon or base you want change to.
  • Knock-in: Select your desired deletion sequence in the sequence map by move the green label and deleted it directly. Then enter the sequence to be inserted.
  • Deletion: Select your desired deletion sequence in the sequence map by move the green label and deleted it directly. You may choose HDR template to increase precise knock out or not according to your request in following step.

Higher score represent the higher on target editing efficiency. Reference: Doench-score.
The ranking order represent overall evaluation according to on/off target score etc.

Higher score represent the higher off target editing efficiency. Reference: Doench-score.
The ranking order represent overall evaluation according to on/off target score etc.