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CRISPR gRNA libraries

- genome-wide and pathway apecific gRNA libraries for efficient screening

- Now in stock and available from just $999 for 25 µg!

CRISPR gRNA library service

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Pooled CRISPR guide RNA libraries, or gRNA libraries, are ideal for high-throughput screening of important molecular targets. These libraries leverage the efficiency and specificity of the CRISPR gene editing technology to either knock-out gene expression or transcriptionally activate genes in the genome.

GenScript offers a variety of gRNA library options, all of which contain sequences pre-validated by the Broad Institute, including (1) GeCKO libraries for genome-wide knockout of human and mouse genes, (2) CRISPR Synergistic Activating Mediator (SAM) libraries for transcriptional activation of every gene in the human and mouse genome, and (3) Pathway-focused gRNA libraries that genes with a specific function.

How are CRISPR libraries used?

Screening using the CRISPR technology is particularly advantageous because of its simplicity, specificity and versatility. The genome-wide GeCKO and SAM libraries were designed to target every gene in the mouse or human genes and knock-out or transcriptionally activate each gene, respectively. Pathway-focused gRNA libraries were designed from targets identified through the Drug Gene Interaction Database, providing application-focused gRNA libraries for more simplified screening. Libraries are amplified, packaged and transduced into host cells to generate mutant cell lines (Figure 1). The mutants can then be screened under specific conditions, such as by positive or negative selection, to identify important genes in a pathway.

Figure 1: How to use CRISPR gRNA libraries

CRISPR gRNA library

CRISPR gRNA libraries at GenScript

GeCKO library for genome-scale knock-out

The genome-scale CRISPR knock-out (GeCKO) v2 libraries enable rapid screening for loss-of-function mutations that produce a desired phenotype in human or mouse cells. GenScript is pleased to offer all eight GeCKO v2 CRISPR libraries developed in Feng Zhang's laboratory through a license with the Broad Institute.* GeCKO libraries are mixed pools of guide RNA targeting each gene and miRNA within the mouse or human genome; the improved GeCKO v2.0 libraries also contain non-targeting gRNA to serve as controls and utilize improved lentiviral vectors for high-titer lentivirus production and highly efficient transduction of primary cells. GeCKO libraries have been used in primary mouse and human cells, stem cells, cancer cells, and stable cell lines. GeCKO library screens allows you to identify genes that are essential for cell viability under different conditions, as well as genes whose loss enables drug resistance in cancer cells. For complete details of GeCKOv2 library design & construction, please see: Sanjana N.E., Shalem O., Zhang F. Improved vectors and genome-wide libraries for CRISPR screening. Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047.

GeCKO v2 libraries are available as two half-libraries, A and B. Each library contains 3gRNAs per gene. Both libraries contain 1,000 non-targeting control sgRNAs. The A library contains gRNAs targeting miRNAs (4 sgRNAs per miRNA) as well. The A and B libraries can be purchased and delivered together to ensure complete representation; however, as more cells are required for both libraries, screening with library A is suggested. For additional information on how to amplify and clone the libraries, please refer to http://genome-engineering.org/gecko/.

Library details

Library Sequence information
Human GeCKO Library A

63,383 sequences:

  • 3 sgRNA targeting each of 19,050 genes;
  • 4 sgRNA targeting each of 1,864 miRNA
  • 1,000 control (non-targeting) sgRNAs
Human GeCKO Library B

58,028 sequences:

  • 3 sgRNA targeting each of 19,050 genes;
  • 1,000 control (non-targeting) sgRNAs
Mouse GeCKO Library A

67,405 sequences:

  • 3 sgRNA targeting each of 20,611 genes;
  • 4 sgRNA targeting each of 1,175 miRNA
  • 1,000 control (non-targeting) sgRNAs
Mouse GeCKO Library B

63,383 sequences:

  • 3 sgRNA targeting each of 20,611 genes;
  • 1,000 control (non-targeting) sgRNAs

The gRNA libraries at GenScript are Industrial Grade and in-stock for immediate delivery (please allow 5-7 days for shipping) unless stated otherwise. Additional quantity options are available, but may require additional turnaround time. Email us for more information on additional library options.

  Catalog # Library Name Vectors Amount Price Quantity


SC1771-25
SC1771-100
SC1771-200
Human GeCKO Library A pLentiCRISPR v2
25 µg
100 µg
200 µg
$4999
$999
$14999
$2999
$24999
$4999




SC1774-25
SC1774-100
SC1774-200
pLentiGuide-Puro
Accompanied by:
pLentiCas9-Blast
25 µg
100 µg
200 µg
$4999
$999
$14999
$2999
$24999
$4999




SC1777-25
SC1777-100
SC1777-200
Human GeCKO Library B pLentiCRISPR v2
25 µg
100 µg
200 µg
$4999
$999
$14999
$2999
$24999
$4999


SC1780-25
SC1780-100
SC1780-200
pLentiGuide-Puro
Accompanied by:
pLentiCas9-Blast
25 µg
100 µg
200 µg
$4999
$999
$14999
$2999
$24999
$4999


SC1783-25
SC1783-100
SC1783-200
Mouse GeCKO Library A pLentiCRISPR v2
25 µg
100 µg
200 µg
$4999
$999
$14999
$2999
$24999
$4999


SC1843-25
SC1843-100
SC1843-200
pLentiGuide-Puro
Accompanied by:
pLentiCas9-Blast
25 µg
100 µg
200 µg
$4999
$999
$14999
$2999
$24999
$4999


SC1849-25
SC1849-100
SC1849-200
Mouse GeCKO Library B pLentiGuide-Puro
Accompanied by:
pLentiCas9-Blast
25 µg
100 µg
200 µg
$4999
$999
$14999
$2999
$24999
$4999


Comment:


GeCKO Library Deliverables

  • Pooled library (Industrial Grade) in 25 µg aliquots. The 100 µg and 200 µg library are delivered as 4 x 25 µg or 8 x 25 µg, respectively. The libraries are delivered with a technical guide, MSDS and COA.
  • Mouse GeCKO Library A (dual vector): 100 µg pooled library (Research Grade)
    • Scale up to 900 µg total, in increments of 100 µg; Industrial Grade available instead of Research Grade
    • Final project report, including ABI files from Sanger sequencing for small-scale tests and statistical summary from next-generation sequencing validation. Complete raw NGS data available upon request

How to use GeCKO libraries

GeCKO libraries are a mixed pool of CRISPR guide RNAs that target every gene and miRNA in the genome. Each gRNA is cloned into a lentiviral vector optimized to produce high-titer virus for efficient lentiviral transduction of primary cells or cultured cell lines. Read More »

How to order:

  • Email us with the (1) catalog number of your desired library and (2) the number of libraries you wish to order.
  • Our technical account representatives will contact you with a quote.
  • Libraries are in-stock (unless specified otherwise) and are available for immediate delivery. Please allow 5-7 business days for shipping.

Have questions? GenScript's Ph.D.-level service representatives are available 24 hours a day, Monday through Friday, to assist you.


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    Phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188
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    Fax: 1-732-210-0262 1-732-885-78
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