Overview

GenScript is proud to offer CRISPR CAR-T knock-in optimization kits to efficiently optimize your CAR insertion process with pre-validated sequences, design and protocols. Save time on optimization by visually confirming the success of your next CAR-T knock-in experiment.

  • Select an insertion site, Cas9 protein and a single or double strand DNA template encoding GFP
  • Follow the knock-in optimization protocol and visualize your result
  • Save 75% compared to purchasing individual reagents

CRISPR CAR-T Knock-In Optimization Kit Includes:

Synthetic SafeEdit sgRNA


HPLC purified guide RNAs targeting 2 commonly used sites for CAR insertion in T cells

High Purity Cas9 Proteins


Available in both wild type and enhanced specificity versions (eSpCas9)

HDR Templates


GenExact ssDNA™ and/or GenWand™ closed-end dsDNA encoding GFP for easy knock-in detection Also available with Cas9 Targeting Sequence (CTS) design*

Build Your Kit!

Choose your desired gene target insertion site:

  • TRAC – Mostly commonly used site for new CAR insertion in T cells, our sgRNA is designed to target the first exon on the TRAC locus
  • Rab11a – Housekeeping gene for more general insertion optimization
  • Other Gene Target – Check out our customized sgRNA and ssDNA/dsDNA Services

Choose your Cas9 protein:

  • Wild type Cas9 protein – The research standard for gene editing with one N-terminal nuclear localization signal (NLS).
  • Enhanced specificity SpCas9 (eSpCas9)-2NLS protein – SpCas9 (K848A/K1003A/R1060A) is structurally engineered with double-ended nuclear localization signals (NLSs) for improved target specificity by Feng Zhang Laboratory at the Broad Institute 1. eSpCas9 is able to reduce off-target effects by over 10-fold, while maintaining robust on-target genome editing efficiency.

Choose your DNA template:

  • GenExact™ ssDNA only
  • GenWand™ dsDNA only
  • GenExact ssDNA and GenWand dsDNA – ideal solution for optimizing HDR DNA templates

Would you like to include the Cas9 Targeting Sequence (CTS) in your DNA template design for improved knock-in efficiency?
See FAQ section below for more details*

  • Yes – Selecting this will add donor templates with CTS design to your test kits, as well as corresponding oligo for the CTS annealing
  • No

Note: Based on our optimized protocol, material in each item labeled for 12 RXN should be enough to cover 12 reactions using Lonza 4D system or 25 reactions using Neon System. Please check your electroporation protocol and ensure enough quantity is added for each item.

Cat. No. Product Name Quantity Price
KIOK1-1.5
SafeEdit TRAC sgRNA - 1.5 nmol
$49.00
KIOK1-3
SafeEdit TRAC sgRNA - 3 nmol
$99.00
KIOK1-6
SafeEdit TRAC sgRNA - 6 nmol
$199.00
KIOK2-1.5
SafeEdit Rab11a sgRNA - 1.5 nmol
$49.00
KIOK2-3
SafeEdit Rab11a sgRNA - 3 nmol
$99.00
KIOK2-6
SafeEdit Rab11a sgRNA - 6 nmol
$199.00
KIOK3-100
Ultra eSpCas9 - 100 μg
$199.00
KIOK3-200
Ultra eSpCas9 - 200 μg
$379.00
KIOK3-400
Ultra eSpCas9 - 400 μg
$749.00
KIOK4-100
Ultra WTSpCas9 - 100 μg
$139.00
KIOK4-200
Ultra WTSpCas9 - 200 μg
$249.00
KIOK4-400
Ultra WTSpCas9 - 400 μg
$499.00
KIOK5
ssDNA_TRAC_EGFP - 50 μg
$999.00
KIOK6
ssDNA_TRAC_EGFP_CTS - 50 μg
$999.00
KIOK7
dsDNA_TRAC_EGFP - 50 μg
$499.00
KIOK8
dsDNA_TRAC_EGFP_CTS - 50 μg
$499.00
KIOK9
ssDNA_RAB11A_GFP - 50 μg
$899.00
KIOK10
ssDNA_RAB11A_GFP_CTS - 50 μg
$899.00
KIOK11
dsDNA_RAB11A_GFP - 50 μg
$449.00
KIOK12
dsDNA_RAB11A_GFP_CTS - 50 μg
$449.00
KIOK13
TRAC CTS Annealing Oligo - 2 nmol
$9.00
KIOK14
RAB11A CTS Annealing Oligo - 2 nmol
$9.00

Note:
KIOK13 TRAC CTS Annealing Oligo is needed for working with KIOK6 ssDNA_TRAC_EGFP_CTS.
KIOK14 RAB11A CTS Annealing Oligo for working with KIOK10 ssDNA_RAB11A_GFP_CTS.

 

Add other items to your cart:

Cat. No. Product Name Quantity Price
KIOK3-100
Ultra eSpCas9 - 100 μg
$199.00
KIOK3-200
Ultra eSpCas9 - 200 μg
$379.00
KIOK3-400
Ultra eSpCas9 - 400 μg
$749.00
KIOK4-100
Ultra WTSpCas9 - 100 μg
$139.00
KIOK4-200
Ultra WTSpCas9 - 200 μg
$249.00
KIOK4-400
Ultra WTSpCas9 - 400 μg
$499.00
 

1.Slaymaker, et al. Rationally engineered Cas9 nucleases with improved specificity. Science 351, 84–88 (2016).
2.Shy, et al. Hybrid ssDNA repair templates enable high yield genome engineering in primary cells for disease modeling and cell therapy manufacturing. bioRxiv 2021.09.02.458799.

FAQ

Resources

Customized Services

Precise and low cytotoxicity GenExact™ ssDNA for efficient CRISPR KI

Ideal for T cell therapy

Ideal for animal model generation

Closed-end GenWand™ dsDNA for long gene knock-in in large scale

Ideal for long gene CRISPR KI

Ideal for screening and scale up

SafeEdit sgRNA with modifications ensuring minimum off-target & cytotoxicity

HPLC purified, >90% purity guaranteed

Ideal for primary cells and stem cell

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