Home » CRISPR Knock-in Comprehensive Guide

CRISPR Resources

Step-by-step Instructions For CRISPR HDR Based Knock-in Experiments

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CRISPR Knock-in Protocol for HEK 293T/293 cells with Thermo Fisher Neon® Transfection System

Free Download
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CRISPR Knock-in Protocol for Jurkat cells with Thermo Fisher Neon® Transfection System

Free Download
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CRISPR Knock-in Protocol for stimulated Human T Cells with Lonza 4D Nucleofector™ X Unit

Free Download
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CRISPR Knock-in Protocol for Stimulated Human T cells with MaxCyte® Electroporation Platform

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Design and Preparation Guidelines for CRISPR KI HDR Templates

(GenExact™ ssDNA/GenWand™ dsDNA/GenCircle™ dsDNA)

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This guideline aims to assist users in designing sgRNA and HDR templates for CRISPR-based gene knockin projects, as well as preparing HDR templates for downstream applications.

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CRISPR Ribonucleoprotein (RNP) User Manual

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A guide on how to use CRISPR RNP for targeted genome editing, Including the following:

  • How to prepare RNA oligos and RNP complex
  • How to test RNP efficacy in cells, through either cationic lipid-mediated transfection or electroporation
  • How to test RNP efficacy in embryos
  • How to test RNP in vitro cutting efficiency
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Need help with sgRNA and KI template design? Check out our Free Online sgRNA and HDR Template Design Tool NEW

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CRISPR Services

CRISPR gRNA Plasmids
CRISPR gRNA Plasmids

Licensed from the Feng Zhang Laboratory at the Broad Institute

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CRISPR sgRNA
CRISPR
sgRNA

A powerful tool for creating gene knock-ins and knock-outs

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HDR Knock-in Templates
HDR Knock-in Templates

ssDNA and dsDNA HDR donor templates for effective gene knock-in

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CRISPR Cell Lines
CRISPR Cell Lines

Genetically modified cell line in any mammalian cell line and targeting any gene.

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Microbial Gene Editing
Microbial Gene Editing

Highly efficient, seamless genome editing in E.coli using CRISPR technology

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CRISPR gRNA Libraries
CRISPR gRNA Libraries

Genome-wide gRNA libraries for efficient screening

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