Home » CRISPR Knock-in Comprehensive Guide

CRISPR Resources

CRISPR Knock-in Electroporation Protocols

molecular-biology-products-flyer

CRISPR Knock-in Protocol for HEK 293T/293 cells with Thermo Fisher Neon® Transfection System

Free Download
molecular-biology-products-flyer

CRISPR Knock-in Protocol for Jurkat cells with Thermo Fisher Neon® Transfection System

Free Download
molecular-biology-products-flyer

CRISPR Knock-in Protocol for stimulated Human T Cells with Lonza 4D Nucleofector™ X Unit

Free Download
molecular-biology-products-flyer

CRISPR Knock-in Protocol for Stimulated Human T cells with MaxCyte® Electroporation Platform

Free Download
molecular-biology-products-flyer

CRISPR Knock-in Protocol for Stimulated Human T-Cells with Thermo Fisher Scientific Neon™ Transfection System

Free Download

CRISPR Lipofection Protocols

molecular-biology-products-flyer

CRISPR Lipofection Protocol for HEK293T/293 cells using Lipofectamine™ 2000 and CRISPRMAX™ Cas9 Transfection Reagents

Free Download

Design and Preparation Guidelines for CRISPR KI HDR Templates

(GenExact™ ssDNA/GenWand™ dsDNA/GenCircle™ dsDNA)

molecular-biology-products-flyer

This guideline aims to assist users in designing sgRNA and HDR templates for CRISPR-based gene knockin projects, as well as preparing HDR templates for downstream applications.

Free Download

CRISPR Ribonucleoprotein (RNP) User Manual

molecular-biology-products-flyer

A guide on how to use CRISPR RNP for targeted genome editing, Including the following:

  • How to prepare RNA oligos and RNP complex
  • How to test RNP efficacy in cells, through either cationic lipid-mediated transfection or electroporation
  • How to test RNP efficacy in embryos
  • How to test RNP in vitro cutting efficiency
Free Download

Need help with sgRNA and KI template design? Check out our Free Online sgRNA and HDR Template Design Tool NEW

Learn More Learn More

CRISPR Services

CRISPR gRNA Plasmids
CRISPR gRNA Plasmids

Licensed from the Feng Zhang Laboratory at the Broad Institute

Learn More
CRISPR sgRNA
CRISPR
sgRNA

A powerful tool for creating gene knock-ins and knock-outs

Learn More
HDR Knock-in Templates
HDR Knock-in Templates

ssDNA and dsDNA HDR donor templates for effective gene knock-in

Learn More
CRISPR Cell Lines
CRISPR Cell Lines

Genetically modified cell line in any mammalian cell line and targeting any gene.

Learn More
Microbial Gene Editing
Microbial Gene Editing

Highly efficient, seamless genome editing in E.coli using CRISPR technology

Learn More
CRISPR gRNA Libraries
CRISPR gRNA Libraries

Genome-wide gRNA libraries for efficient screening

Learn More

REQUEST A QUOTE

EMAIL

[email protected]

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission