CRISPR Knock-in Electroporation Protocols
CRISPR Knock-in Protocol for HEK 293T/293 cells with Thermo Fisher Neon® Transfection System
Free Download
CRISPR Knock-in Protocol for Jurkat cells with Thermo Fisher Neon® Transfection System
Free Download
CRISPR Knock-in Protocol for stimulated Human T Cells with Lonza 4D Nucleofector™ X Unit
Free Download
CRISPR Knock-in Protocol for Stimulated Human T cells with MaxCyte® Electroporation Platform
Free Download
CRISPR Knock-in Protocol for Stimulated Human T-Cells with Thermo Fisher Scientific Neon™ Transfection System
Free DownloadCRISPR Lipofection Protocols
CRISPR Lipofection Protocol for HEK293T/293 cells using Lipofectamine™ 2000 and CRISPRMAX™ Cas9 Transfection Reagents
Free DownloadDesign and Preparation Guidelines for CRISPR KI HDR Templates
(GenExact™ ssDNA/GenWand™ dsDNA/GenCircle™ dsDNA)
This guideline aims to assist users in designing sgRNA and HDR templates for CRISPR-based gene knockin projects, as well as preparing HDR templates for downstream applications.
Free DownloadCRISPR Ribonucleoprotein (RNP) User Manual
A guide on how to use CRISPR RNP for targeted genome editing, Including the following:
Need help with sgRNA and KI template design? Check out our Free Online sgRNA and HDR Template Design Tool NEW
Licensed from the Feng Zhang Laboratory at the Broad Institute
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A powerful tool for creating gene knock-ins and knock-outs
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ssDNA and dsDNA HDR donor templates for effective gene knock-in
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Genetically modified cell line in any mammalian cell line and targeting any gene.
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Highly efficient, seamless genome editing in E.coli using CRISPR technology
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Genome-wide gRNA libraries for efficient screening
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