GenCRISPR Cas13a (C2c2) Nuclease is an RNA-guided, RNA-targeting CRISPR enzyme, commonly referred to as C2c2. This enzyme is a member of Type VI CRISPR family containing a single protein effector with two HEPN domains. The Cas13a exhibits two RNase activities. The first activity, a cis- sequence-specific RNA guided cleavage, activates the secondary RNase activity of the enzyme, a non-specific trans- ribonuclease activity. This Cas13a protein can be applied to detect single RNA molecules with proven high specificity.
Recently, Cas13a has been leveraged as a diagnostic nucleic acid detection tool. Using a combination of the Cas13a cis- and trans- RNase activity and a non-target RNA tagged with a cleavage reporter ((fluorescence), the Cas13 system provides a route for reporting of the presence of a specific single RNA sequence in a sample with high specificity.
The GenScript Cas13a contains a His-tag at the C-terminal.
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
crRNA-dependent RNA cleavage
Cas13a expressed by E.coli
C-terminal His Tag
|Apparent Molecular Weight||
kDa, on SDS-PAGE under non-reducing conditions
refer to the COA for the specific lot.
as a solution of 10 mM Tris, pH 8.0, 300 mM
NaCl, 1.0 mM DTT, and 50% glycerol.
|Storage & Stability||This product remains stable up to 12 months at -20℃. Avoid repeated freeze-thaw cycles.|
|Quality Control Specifications||
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