Catalog Products » GenCRISPR™ Cas9 Genome Editing Products » GenCRISPR™ Nucleases » GenCRISPR™ Ultra eSpCas9-2NLS-Research
GenCRISPR™ Ultra ESpCas9-2NLS-Research

A 20 μl reaction in 1×Cas9 Nuclease Reaction Buffer containing 160 ng linearized plasmid, 100 ng gRNA, and 50 ng GenCRISPR™ Ultra eSpCas9-2NLS for 2 hours at 37°C results in a digestion efficiency of linearized plasmid higher than 90%, as determined by agarose gel electrophoresis.

GenCRISPR™ Ultra eSpCas9-2NLS-Research

GenCRISPR™ Ultra eSpCas9-2NLS is a mutant form of Cas9 nuclease and is produced by expression in an E. coli strain carrying a plasmid encoding the eSpCas9 gene from Streptococcus pyogenes with double-ended nuclear localization signal (NLS). GenCRISPR™ Ultra eSpCas9-2NLS delivers higher fidelity and less off-target activity than wild-type SpCas9 nuclease.
Z03622
$765.00

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Description

The GenCRISPR™ Ultra eSpCas9 product line provides customers with a selection of research use, basic GMP and GMP compliant Cas9 nucleases. The Cas9 protein can be formed with the guide RNA into a ribonucleoprotien (RNP) complex. The use of an RNP complex to perform gene editing has been shown to reduce the challenges encountered with other CRISPR gene editing techniques such as viral and plasmid delivery. Challenges include off-target effects, cell viability and transcription/translational challenges.


GenCRISPR™ Ultra eSpCas9-2NLS is a mutant form of Cas9 nuclease and is produced by expression in an E. coli strain carrying a plasmid encoding the eSpCas9 gene from Streptococcus pyogenes with double-ended nuclear localization signal (NLS). GenCRISPR™ Ultra eSpCas9-2NLS delivers higher fidelity and less off-target activity than wild-type SpCas9 nuclease.

Note

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

Applications
gRNA-dependent double-stranded DNA cleavage

Properties
Expression System Recombinant Cas9 with double-ended NLS expressed by E.coli
Species S. pyogenes
Tag Tag-free
Molecular Weight ~160 kDa
Concentration 10 mg/mL
Active temperature This eSpCas9 is active at 37°C.
Formulation Supplied as a solution of 25 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 50% Glycerol pH 8.0 at 25°C. 
Storage & Stability This product remains stable up to 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Key Features High knockout efficiencies: Consistent high performance in in-vitro plasmid cleavage test.
Tag-free: No extra tag amino acid
Animal-free: Plant-based culture media, Enzyme-free chromatographic purification.
DNA-free: No external DNA added to the system.

Quality Control Specifications
Appearance Clear, colorless liquid
Purity ≥ 95% as analyzed by SDS-PAGE
≥ 90% as analyzed by SEC-HPLC
Concentration by A280 10 mg/ml±1 mg/ml
Bioactivity (in vitro) ≥ 90%
Residual DNase Non-specific DNase activity
Residual RNase Non-specific RNase activity
Endotoxin Level ≤ 10 EU/mg as analyzed by gel clotting method

Examples
  • GenCRISPR™ Ultra ESpCas9-2NLS-Research
  • GenCRISPR™ Ultra ESpCas9-2NLS-Research

    A 20 μl reaction in 1×Cas9 Nuclease Reaction Buffer containing 160 ng linearized plasmid, 100 ng gRNA, and 50 ng GenCRISPR™ Ultra eSpCas9-2NLS for 2 hours at 37°C results in a digestion efficiency of linearized plasmid higher than 90%, as determined by agarose gel electrophoresis.


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