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PE6d

PE6d

PE6d

PE6d is designed to optimize the efficiency of prime editing by altering certain components of the prime editor framework. It incorporates elements that minimize the chances of errors and maximize successful genomic edits across various cell types. This improvement results in enhanced editing accuracy and reduced byproducts, such as unintended indels.
RC00008
$150.00

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Overview
Description PE6d is designed to optimize the efficiency of prime editing by altering certain components of the prime editor framework. It incorporates elements that minimize the chances of errors and maximize successful genomic edits across various cell types. This improvement results in enhanced editing accuracy and reduced byproducts, such as unintended indels.

Applications
single guide RNA (sgRNA) dependent single-stranded DNA cleavage

Background
References David R Liu, et al. “Phage-assisted evolution and protein engineering yield compact, efficient prime editors”. Cell. 2023 Aug 31;186(18):3983-4002.e26.

Quality Control Specifications
Purity ≥90% by SDS-PAGE, 97% by SEC-HPLC
Residual DNase Non-specific DNase activity
Endotoxin Level Control <10EU/mg

Properties
Expression System Recombinant Cas9-based fusion with three NLS expressed by E.coli
Species S. pyogenes
Tag C-His tag
Molecular Weight 223.780 KDa
Storage & Stability PE6d is supplied with storage buffer (50 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol pH7.5, at 25°C) and recommended to be stored at -80°C.

Examples
  • PE6d
    • PE6d

  • PE6d
    • PE6d