Catalog Products » GenCRISPR™ NLS-wtSpCas9, his-tag
GenCRISPR™ NLS-wtSpCas9, His-tag

Figure 1. Cas9 cuts substrate DNA standard via in vitro cutting experiment. Results show the cleavage activity of GenScript Cas9 is equivalent to that of leading competitors.

GenCRISPR™ NLS-wtSpCas9, His-tag

Figure 2. The purity of GenScript Cas9 detected by SEC-HPLC is greater than 95%.

GenCRISPR™ NLS-wtSpCas9, His-tag

Figure 3. The purity of GenScript Cas9 detected by Tris-Bis-PAGE is greater than 95%.

GenCRISPR™ NLS-wtSpCas9, His-tag

Figure 4. In vitro cleavage assays showed that the in vitro cleavage activity of Cas9 Nuclease was consistent across three batches.

GenCRISPR™ NLS-wtSpCas9, His-tag

Figure 5. Cas9 is electrotransfected into 293T cell line in the form of RNP to knock out the target gene. Results show GenScript Cas9 has the same knockout efficiency as leading competitors.

GenCRISPR™ NLS-wtSpCas9, his-tag

This newly wtSpCas9 is produced by expression in an E. coli strain carrying a plasmid encoding the Cas9 gene from Streptococcus pyogenes with an nuclear localization signal (NLS) and his-tag.
RC00001
$150.00

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Description

CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and Cas9.

Note

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

Applications
Genome editing with CRISPR T-Cells, Stem Cells, etc.  

Quality Control Specifications
Appearance Clear liquid
Purity ≥ 95.0% by SEC-HPLC, ≥ 95.0% by Bis-Tris PAGE
Quality Antibiotic-free and animal-free production
Concentration 9.5-12.5 mg/mL  
Bioactivity cleavage activity>85%  
Residual RNase Negative  
Endotoxin Level ≤ 10.0EU/mg  

Properties
Synonyms CRISPR-associated endonuclease Cas9/Csn1, cas9, SpCas9, SpyCas9
Source This product has undergone codon optimization, nuclear localization signal (NLS) design, and optimization of E. coli expression and purification.  
Species Streptococcus pyogenes
Tag His tag
Molecular Weight 163 kDa
Concentration 10 mg/ml
Active temperature This Cas9 is active at 37°C.
Formulation Supplied as a solution of 30mM Tris-HCl, 0.3M NaCl, 50% Glycerol, 0.1 mM EDTA, pH 7.4
Storage & Stability This product remains stable for up to 18 months at -20±5°C. Avoid repeated freeze-thaw cycles.

Examples
  • GenCRISPR™ NLS-wtSpCas9, His-tag
  • GenCRISPR™ NLS-wtSpCas9, His-tag

    Figure 1. Cas9 cuts substrate DNA standard via in vitro cutting experiment. Results show the cleavage activity of GenScript Cas9 is equivalent to that of leading competitors.

  • GenCRISPR™ NLS-wtSpCas9, His-tag
  • GenCRISPR™ NLS-wtSpCas9, His-tag

    Figure 2. The purity of GenScript Cas9 detected by SEC-HPLC is greater than 95%.

  • GenCRISPR™ NLS-wtSpCas9, His-tag
  • GenCRISPR™ NLS-wtSpCas9, His-tag

    Figure 3. The purity of GenScript Cas9 detected by Tris-Bis-PAGE is greater than 95%.

  • GenCRISPR™ NLS-wtSpCas9, His-tag
  • GenCRISPR™ NLS-wtSpCas9, His-tag

    Figure 4. In vitro cleavage assays showed that the in vitro cleavage activity of Cas9 Nuclease was consistent across three batches.

  • GenCRISPR™ NLS-wtSpCas9, His-tag
  • GenCRISPR™ NLS-wtSpCas9, His-tag

    Figure 5. Cas9 is electrotransfected into 293T cell line in the form of RNP to knock out the target gene. Results show GenScript Cas9 has the same knockout efficiency as leading competitors.