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GenScript is the trusted choice for scientists worldwide, offering flexible, high-quality circular RNA synthesis solutions. Our proprietary circRNA vectors enables high circularization efficiency and purity.with customizable designs support to support your research needs. Scalable from 100 μg to 15 mg, ≥ 80% HPLC purified delivered in three weeks. We ensure precision , reliability, and high-performance RNA. The result? Enhanced RNA stability, reduced immunogenicity, prolonged protein expression, and superior translation efficiency, driving innovation in research and therapeutics.
Select the method best suited for your target application
At GenScript, we provide three circRNA custom synthesis methods, ensuring the precise solution your research demands. Each method offers specific advantages tailored to different applications.
circRNA with Scar by  |
Scarless circRNA by
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Scarless circRNA by  |
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| Application | For protein translation in coding applications | For protein translation in coding applications w/ improved stability and protein expression vs. circRNA with scar | For noncoding RNA applications |
| Description | Generates circRNA with a small scar sequence | Generates circRNA with a small scar sequence | Uses T4 ligase for seamless circularization |
| Total Sequence Size | 5 kb | 5 kb | 2 kb |
| Final circRNA sequence | IRES+Exon+Linker (Scar) Sequence+ORF | IRES+ORF | Customer provided sequence |
Protein-coding application:
Non-coding application:
| QC | Item | Test Method | Specification | RUO Standard QC |
RUO Upgrade |
|---|---|---|---|---|---|
| Identification | Appearance | Visual inspection | Clear and free of foreign particles | ||
| RNA length | Agarose gel electrophoresis | Expected size band detected | |||
| Identification | RNaseR degradation test | Not be degraded by RNase R | |||
| RNA content | UV absorbance | Target ± 5% | |||
| pH | pH paper | Target ± 0.5 | |||
| Buffer specification | Client spec | N/A | |||
| Purity | A 260/280 ratio | UV Spec | 1.70 ~ 2.30 | ||
| Purity by HPLC | HPLC | ≥80% | |||
| Impurity | Total protein residue | NanoOrange assay | ≤ 1% | ||
| Plasmid DNA residue | qPCR | ≤ 0.1% | |||
| Safety | Endotoxin | Semiquantitative | < 10 EU/mg | ||
| Endotoxin | Quantitative | < 10 EU/mg | |||
| Bioburden | Direct inoculation | No growth after 48 hrs |
The translation efficiency and stability of different RNA formats (Linear mRNA, circ-Scar, and circ-Scarless) in two cell lines: HEK293T (human embryonic kidney cells) and Jurkat cells (human T lymphocyte cells). Superior expression compared to circ-Scar. Expression peaks at 12 hours and remains high, with gradual decline after 6 days, but still outperforms both Circ-Scar and linear mRNA.
RNA Copy Number by RT-PCR
RNA Expression by Fluorescence Intensity
Circular eGFP- RNA (nonmodified) and Linear eGFP-mRNA (100% N1-me-pseU modified) were transfected with equal molar quantity in A549 cells. Coding RNA abundance was measured by qPCR, and GFP intensity was measured by flow cytometry. Results demonstrated that circRNA has higher stability, slower degradation, compared to linear IVT mRNA. circRNA also exhibits higher protein expression level and duration compared to linear IVT mRNA
GenScript circRNA demonstrates lower immunogenicity than mRNA. For IL6 test, ELISA method was used Abcam Human IL-6 ELISA Kit (ab46027). For INF-a and NF-kb test, A549 dual cells (InvivoGen) were transfected with test mRNA, and the expression of INF-a in cells was detected by relative luminescence testing for ISG54 activity following QUANTI-Luc/Gaussia kit instruction. The NF-Kb level was detected by OD at 630nm of cell medium following QUANTI-Blue kit instruction.
GenScript circRNA shows higher initial expression and cumulative expression than both mRNA and circular RNA prepared with a published method. A549 cells were transfected with circRNAs and mRNA encoding eGFP. circRNAs were prepared using GenScript’s proprietary method and using methods published in Nat Commun 9, 2629 (2018). Fluorescence was analyzed via flow cytometry for up to 6 days following transfection (10,000 cells were analyzed per condition at each time point). A549 cell doubling time = 22hr.
GenScript HPLC-purified circRNA shows higher expression than both crude circRNA and circRNA prepared with a published method. Cells were transfected with circRNAs encoding eGFP prepared using GenScript’s proprietary method and using methods published in Nat Commun 9, 2629 (2018). Fluorescence was analyzed via flow cytometry.
Popular off-the-shelf circRNA catalogs available now!
Try our catalog mRNA expressing eGFP or F-Luciferase, delivered in just 5 days! Learn More >>
Circular RNA introduction, GenScript offering details, and case studies showing increased stability, prolonged expression, and lower immunogenicity compared to linear IVT mRNA.
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Learn about key structural components of circRNA, the process by which circRNA is translated into protein, and more.
Free DownloadScarless circRNA eliminates unwanted sequence scars, resulting in a more natural RNA structure with improved stability and efficiency. circRNA with Scar includes strong IRES activity and effective expression in various models.
GenScript’s circRNA synthesis process features proprietary circularization techniques and HPLC purification, ensuring the highest purity and RNA integrity.
Absolutely! We offer cell-specific IRES options optimized for various applications, including gene therapy and functional studies.
circRNA offers enhanced stability, prolonged protein expression, lower immunogenicity, and reduced degradation, making it ideal for advanced therapeutic applications.
The turn around time for circular RNA production is 3 weeks. Overall turn around time for circular RNA from gene synthesis to circular RNA will be about 7 weeks.
We support insert sequences upto 5Kb. For custom lengths, please contact our technical team.
Yes, we offer T4-based ligation methods to ensure completely scarless circular RNA.
Our QC test for circular RNA include agarose gel electrophoresis assay, PH, endotoxin assay. We have add on QC for testing the circular RNA purity by HPLC method, or RNaseR digestion assay as identification assay.
Yes, modifications are available such as 5% M6A. Please consult our technical team for further customizations.
High-quality custom mRNA production via our proprietary IVT RNA manufacturing workflow.
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Our off-the-shelf mRNA is optimized with 5’ cap and polyA tails that is ready to use for your research needs
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LNP Packaging
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Send us your in house mRNA samples for expert QC analysis.
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