Discover A New Frontier in Vaccine Development

Self-amplifying RNA (saRNA) is a type of mRNA that encodes a replicase to copy the original RNA strand within the cell. It shares many structural similarities with conventional mRNA, such as being a linear, single-stranded RNA molecule synthesized with a 5′ cap, 3′ polyA tail, and 5′ and 3′ untranslated regions (UTRs). Unlike conventional mRNA, saRNA offers a promising avenue for low-dosage, reduced side effects, and long-lasting vaccine outcomes.

GenScript is proud to be one of the first saRNA suppliers that offers tailored design and modifications with reliable quality to help speed up your innovative research projects!

Benefits of saRNA

Market Leader for Optimized Production

Market Leader for Optimized Production

Ready-to-use template (Patent filed) & fully optimized production process

Enhanced & Prolonged Expression

Less quantity needed for strong and lasting expression

Optimized Vectors for Diverse Applications

Vectors with varying degrees of immunogenicity available for different applications

How does saRNA work?

Comparison of different IVT RNA payload formats

Linear mRNA Self-Amplifying RNA Circular RNA
Structure Linear; Open 5’ and 3’ end
mRNA exonuclease degradation
Linear; Open 5’ and 3’ end
mRNA exonuclease degradation
Close loop; No free 5’ 3’ end
Eliminating mRNA exonuclease degradation
Ribosome recruiting 5’ Cap:
Initiates translation & stabilizes mRNA
5’ Cap:
Initiates translation & stabilizes mRNA
Internal Ribosome Entry Site (IRES) :
Initiates translation
ORF
  • Codon optimization for translation yield
  • Modified nucleosides, eg. M1Ψ
  • Insert < 20 kb
  • Codon optimization for translation yield
  • Modified nucleosides, eg. M5C
  • Insert < 6 kb
  • Codon optimization for translation yield
  • Insert < 4 kb
UTR Regulatory elements in the 5’ and 3’-UTR stabilize mRNA and increase translation initiation Replicon for further self-replication of the mRNA Include IRES + PIE + other elements
PolyA tail Stabilizes RNA molecule & Prevents degradation Stabilizes RNA molecule & Prevents degradation Not required
Protein Expression +++ Strong +++++ Strongest ++++ Stronger
Expression half-life +++ Shorter +++++ Longest ++++ Longer
Immunogenicity ++++ Low ++ High +++ Medium
Dosage requirement ++ High +++++ Lowest +++ Medium
Novelty +++ Traditional ++++ Novel +++++ Novel

Service

Codon/UTR
Optimization
  • Codon optimization for protein expression offered every day for every gene
  • Codon optimization with >200 factors screened (patent)
Gene
Synthesis
  • 100 bp – 6 kb gene inserts
  • TAT as fast as 6 BDs to 14 BDs
  • Gene synthesis with Poly(A)-built-in vector
Cloning/Plasmid
Synthesis
  • Ready to use linearized plasmid DNA for IVT
  • Vectors encoded stable Poly(A) tracts with variance of only +/- 5-10 nt
saRNA
Synthesis
  • Customized RUO mRNA service
  • Advanced purification and variety of QC options
Lipid Nanoparticles
Delivery
  • Generic ionizable lipid formulation
  • Load 1-30 mg saRNA

GenScript saRNA offers the flexibility to meet your project needs

Quality Control and Specifications

QC Item Test Method Specification RUO
Standard
RUO
Upgrade
Identification Appearance Visual inspect Clear and free of foreign particles​
RNA length​ Capillary electrophoresis ​ Target ± 30%
RNA length​ Agarose gel electrophoresis​ Expected size band detected​
Poly(A) length​​ Enzyme digestion and  CE Target ± 30%​
RNA content​ UV absorbance​ Target ± 5%​
PH pH meter Target ± 0.5
Buffer specification​ Client spec N/A
Purity A 260/280 ratio​ UV spec 1.70 ~ 2.30
Capping efficiency​ LC-MS ≥ 90%
Size-based purity​ Capillary electrophoresis ​ ≥ 70%
Safety Endotoxin​ Semiquantitative​ < 10 EU/mg​

Case Studies

Resources

Self-Amplifying RNA Beginner’s Guide_Infographic
Self-Amplifying RNA Beginner’s Guide_Infographic

Learn about key structural components of saRNA, the processes by which saRNA replicates and is translated into protein, and more.

Free Download

FAQs

Additional mRNA Offerings

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