RNA Copy Number by RT-PCR

RNA Expression by Fluorescence Intensity

Circular eGFP- RNA (nonmodified) and Linear eGFP-mRNA (100% N1-me-pseU modified) were transfected with equal molar quantity in A549 cells. Coding RNA abundance was measured by qPCR, and GFP intensity was measured by flow cytometry. Results demonstrated that circRNA has higher stability, slower degradation, compared to linear IVT mRNA. circRNA also exhibits higher protein expression level and duration compared to linear IVT mRNA