Result: More than 80% editing observed at the TRAC locus
Method: espCas9-mRNA : SafeEdit TRAC sgRNA were co-encapsulated in LNP at a molar ratio of 1:10 , 2.4 ug of RNA loaded LNP were incubated with cells for 72 hours, the gene editing efficiency for knocking out TRAC locus was sanger sequenced and editing efficiency was analyzed by ICE Analysis tool Software