Overview

GenCRISPR synthetic sgRNAs are proven effective for targeted gene editing in knockout and HDR mediated knock-in experiments. Combining sgRNA with Cas9 protein into a ribonucleoprotein (RNP) complex offers additional advantages over traditional plasmid or virus based delivery systems.

Additionally, we offer custom synthesis of pegRNA for prime editing and crRNA for Cpf1/Cas12a editing systems.

Use the gRNA Design Tool to design and order guide RNA and DNA templates for your CRISPR/Cas9 knockout or HDR knock-in experiments – Simply select your experiment type to get started.

sgRNA Services – Quality to meet your needs

EasyEdit

Synthetic sgRNAs with modifications for reliable gene editing
1
SafeEdit

HPLC purified for improved performance
2
cGMP/GMP-like

 Supporting IND filing and clinical trials
3

EasyEdit

icon-trumpetSynthetic EasyEdit sgRNAs now starting at only $79/2nmol!

✔ Option to deliver in 96-well plates supporting high throughput screening

Most economical solution

Ideal for early research & discovery

High editing efficiency

Regardless of cell type

Flexible at scale

1 to 1000+ sgRNAs 2 to 100+ nmol

Why choose EasyEdit sgRNA?

Reliable and Consistent Editing Results

GenScript EasyEdit sgRNA achieved >80% KO efficiency for all three gene targets tested, comparable to other vendors. sgRNAs were designed for 3 randomly selected gene targets (Seq_1, Seq_2, Seq_3), and KO efficiency of sgRNAs from different vendors were examined in Jurkat cells.

Superior purity compared to competitor products

GenScript EasyEdit sgRNA demonstrates higher purity compared to competitor products. UPLC analysis of sgRNA purity from different vendors for sgRNAs designed for 3 randomly selected gene targets (Seq_1, Seq_2, Seq_3).

Higher % of full-length sgRNA sequences
compared to competitor products

GenScript EasyEdit sgRNA exhibited minimal non-specific peaks in MS analysis (a) and a higher percentage of full-length sgRNA sequences in NGS analysis (b) compared to competitor products for the same sgRNA sequence design.

Pricing

Product Quantity Pricing Quote
EasyEdit sgRNA 2 nmol $79 Quote
4 nmol $149
10 nmol $329
50 nmol $999
100 nmol $1,299

Add-On Items

Product Quantity Pricing Quote
Custom Primer for Assessing Editing Efficiency 2 nmol $1.2/nt Quote
EasyEdit Human HPRT Positive Control sgRNA 2 nmol $39 Quote
SafeEdit Human HPRT Positive Control sgRNA 2 nmol $69 Quote
Human HPRT Primer Mix for Assessing Editing Efficiency 2 nmol $10 Quote
GenCRISPR™ Ultra NLS-Cas9 Nuclease (10 mg/ml) 500 μg $695 Quote
1 mg $1181.50
GenCRISPR™ Ultra eSpCas9-2NLS Nuclease (10 mg/ml) 500 μg $1245 Quote
1 mg $2116.50
Long Single-Stranded DNA Synthesis for Gene Knock-In (151-5000 nt) 3 μg and up Starting at $0.8/nt Quote

EasyEdit
The easy yet effective one-step solution
Starting at $79/2nmol!

HPLC purified sgRNAs minimize off-targeting and cytotoxicity

✔ More than 90% purity guaranteed

Minimal impact for
cell viability

Reduced off-targeting from
truncated guides

Ideal for primary cells
& stem cells

Why choose SafeEdit sgRNA?

HPLC purified sgRNA eliminates off-targeting from truncated oligos

High purity SafeEdit sgRNA ensures minimal impact for cell viability

GenScript provides add-on QC options to ensure sustained quality of your cells:

  • Mycoplasma testing (qPCR)
  • Endotoxin testing (Kinetic LAL)
  • Stability testing
  • Cytotoxicity testing (co-culture with client specified cell line)

Pricing

Product Quantity Pricing Quote
SafeEdit sgRNA 2 nmol $199 Quote
4 nmol $319
10 nmol $579
50 nmol $1,699
100 nmol $2,499

Add-On Items

Product Quantity Pricing Quote
Custom Primer for Assessing Editing Efficiency 2 nmol $1.2/nt Quote
EasyEdit Human HPRT Positive Control sgRNA 2 nmol $39 Quote
SafeEdit Human HPRT Positive Control sgRNA 2 nmol $69 Quote
Human HPRT Primer Mix for Assessing Editing Efficiency 2 nmol $10 Quote
GenCRISPR™ Ultra NLS-Cas9 Nuclease (10 mg/ml) 500 μg $695 Quote
1 mg $1181.50
GenCRISPR™ Ultra eSpCas9-2NLS Nuclease (10 mg/ml) 500 μg $1245 Quote
1 mg $2116.50
Long Single-Stranded DNA Synthesis for Gene Knock-In (151-5000 nt) 3 μg and up Starting at $0.8/nt Quote

cGMP/GMP-like
Supporting IND filing and clinical trials

GenScript now offers full cGMP and GMP-like sgRNA

✔ Supporting IND filing and clinical trials

State-of-the-art production facility

Clean suite with class A isolator in a class C background

Comprehensive QA/QC

Proprietary NGS method to verify sgRNA identity

Trusted by scientific partners globally

Successfully delivered 30+ cGMP grade batches

Prime Editing Guide RNA (pegRNA)

Prime editing (PE) is a novel gene editing technique that allows for precise and efficient DNA editing. The system combines an engineered prime editor protein, containing a modified Cas9 nuclease fused to a reverse transcriptase enzyme, with a PE guide RNA (pegRNA) capable of both identifying the target site and, encoding new genetic sequence. This allows the enzyme to not only cut the DNA at a specific location, but also to copy a new DNA sequence into the cut site.

GenScript supplies long custom guide RNAs for applications such as Prime Editing (pegRNA). Our expert RNA synthesis group regularly delivers projects up to 180nt at scale and purity levels to meet our customers needs.

Simply enter your full pegRNA sequence to receive a quote or contact us for design help.

Cas12a/Cpf1 Guide RNA (crRNA)

Cas12a (also known as Cpf1) nuclease can be paired with a specifically designed guide RNA (crRNA) to target and cut DNA sequences in a staggered pattern, producing a cohesive end, which can make it easier to insert new genetic material into the cut site.

GenScript offers custom synthesis of Cas12a crRNA, simply follow the link to receive a quote or contact us for design help.

Resources

CRISPR/Cas9 gRNA Vector
RNP user manual

A guide on how to use CRISPR RNP for targeted genome editing.

Free Download
floatL
CRISPR Knock-in Comprehensive Guide

Step-by-step instructions for your CRISPR knock-in experiments.

Free Download

FAQs

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with GenScript CRISPR Experts

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