GenCRISPR Synthetic sgRNA

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Overview

GenCRISPR synthetic sgRNAs are proven effective for targeted gene editing in knockout and HDR mediated knock-in experiments. Using sgRNA with Cas9 protein in the ribonucleic protein (RNP) system offers additional advantages over traditional plasmid or virus based delivery systems.

Advantages

Ready-to-use sgRNA, no IVT or annealing required

Detectable at high levels shortly after transfection

DNA free / transgene-free

Quickly cleared from the cell for less off-target effects

Suitable for both ex and in vivo studies

Highly efficient even in difficult primary cell types

Available in multiple grades to support your editing needs

EasyEdit sgRNA

The easy yet effective one-step solution

Synthetic sgRNAs with modifications supporting easy and effective gene editing

✔ Option to deliver in 96-well plates supporting high throughput screening

Most economical solution

Ideal for early research & discovery

High editing efficiency

Regardless of cell type

Flexible at scale

1 to 1000+ sgRNAs 2 to 100+ nmol

Design Custom Guides Order Your Own Design Request Now

Why choose EasyEdit sgRNA? »

Synthetic sgRNA provides much better editing efficiency compared to IVT sgRNA
Did we mention that preparing IVT sgRNA from plasmid template takes ~4 hours in-house?

Pricing »

Product	Quantity	Pricing	Quote
EasyEdit sgRNA	2 nmol	$99	Quote
	4 nmol	$149
	10 nmol	$259
	50 nmol	$799
	100 nmol	$1,199

Add-On Items

Product	Quantity	Pricing	Quote
Custom Primer for Assessing Editing Efficiency	2 nmol	$1.2/nt	Quote
EasyEdit Human HPRT Positive Control sgRNA	2 nmol	$39	Quote
SafeEdit Human HPRT Positive Control sgRNA	2 nmol	$69	Quote
Human HPRT Primer Mix for Assessing Editing Efficiency	2 nmol	$10	Quote
GenCRISPR™ Ultra NLS-Cas9 Nuclease (10 mg/ml)	500 μg	$695	Quote
GenCRISPR™ Ultra NLS-Cas9 Nuclease (10 mg/ml)	1 mg	$1181.50	Quote
GenCRISPR™ Ultra eSpCas9-2NLS Nuclease (10 mg/ml)	500 μg	$1245	Quote
GenCRISPR™ Ultra eSpCas9-2NLS Nuclease (10 mg/ml)	1 mg	$2116.50	Quote
Long Single-Stranded DNA Synthesis for Gene Knock-In (151-5000 nt)	3 μg and up	Starting at $0.8/nt	Quote

SafeEdit sgRNA

Safeguard your cells

HPLC purified high purity sgRNAs with modifications ensuring minimum off-target and cytotoxicity

More than 90% purity guaranteed

Minimal impact for
cell viability

Reduced off-targeting from
truncated guides

Ideal for primary cells
& stem cells

Design Custom Guides Order Your Own Design Request Now

Why choose SafeEdit sgRNA? »

HPLC purified sgRNA eliminates off-targeting from truncated oligos

High purity SafeEdit sgRNA ensures minimal impact for cell viability

GenScript provides add-on QC options to ensure sustained quality of your cells:

Mycoplasma testing (qPCR)
Endotoxin testing (Kinetic LAL)
Stability testing
Cytotoxicity testing (co-culture with client specified cell line)

Pricing »

Product	Quantity	Pricing	Quote
SafeEdit sgRNA	2 nmol	$199	Quote
	4 nmol	$319
	10 nmol	$579
	50 nmol	$1,699
	100 nmol	$2,499

Add-On Items

Product	Quantity	Pricing	Quote
Custom Primer for Assessing Editing Efficiency	2 nmol	$1.2/nt	Quote
EasyEdit Human HPRT Positive Control sgRNA	2 nmol	$39	Quote
SafeEdit Human HPRT Positive Control sgRNA	2 nmol	$69	Quote
Human HPRT Primer Mix for Assessing Editing Efficiency	2 nmol	$10	Quote
GenCRISPR™ Ultra NLS-Cas9 Nuclease (10 mg/ml)	500 μg	$695	Quote
GenCRISPR™ Ultra NLS-Cas9 Nuclease (10 mg/ml)	1 mg	$1181.50	Quote
GenCRISPR™ Ultra eSpCas9-2NLS Nuclease (10 mg/ml)	500 μg	$1245	Quote
GenCRISPR™ Ultra eSpCas9-2NLS Nuclease (10 mg/ml)	1 mg	$2116.50	Quote
Long Single-Stranded DNA Synthesis for Gene Knock-In (151-5000 nt)	3 μg and up	Starting at $0.8/nt	Quote

Preclinical grade sgRNA
Extra Purification and Quality control

For preclinical in vivo and ex vivo studies
- Extra C18 desalt purification after HPLC to remove inorganic salts
Sterile fill and packaging

CoA and TSE/BSE Certification

Delivery in 1-3 weeks

Request a Quote
cGMP Grade sgRNA

GenScript now offers cGMP sgRNA- Dedicated for IND and clinical phase I material production:

State-of-the-art production facility

Clean suite with class A isolator in a class C background

Comprehensive QA/QC

Proprietary NGS method to verify sgRNA identity

Trusted by scientific partners globally

Successfully delivered 30+ cGMP grade batches

Learn More Request a Quote

Resources

Why CRISPR/Cas9 RNP system?
Unlike the traditional plasmids or lentivirus delivery methods, CRISPR/Cas9 RNP are delivered as intact complexes, and do not require cellular expression, thus has many advantages.
- DNA free
- Detectable at high levels shortly after transfection
- Quickly cleared from the cell for less off-target effects
- Highly efficient even in hard-to-transfect cells
- Best for in vivo studies
Recently, several studies have showed that sgRNA has better stability than crRNA:tracrRNA when duplexed with Cas9, thus leading to higher editing efficiency. Synthesis of 100 nt long sgRNAs was traditionally possible through in vitro-transcription (IVT) using phage RNA polymerase. These in vitro transcribed sgRNAs contain a 5’-triphosphate, which was thought to trigger immune response in many cell types. A recent study showed that sgRNAs with 5’-triphosphate modifications produced through in vitro-transcription can indeed induce innate immune responses and lead to cytotoxicity in human and murine cells. However, chemically synthesized sgRNAs without the 5’-triphosphate modifications demonstrated much better editing efficiency in cells, thus supporting that chemically synthesized sgRNAs are the most ideal reagent for CRISPR genome editing up till now¹.

Kim et al. CRISPR RNAs trigger innate immune responses in human cells. Genome Res. 2018. 28: 367-373.
CRISPR Handbooks and Protocols

CRISPR Handbook
Enabling Genome Editing and Transforming Life Science Research
Free Download

RNP user manual
A guide on how to use CRISPR RNP for targeted genome editing.
Free Download

CRISPR Knock-in Comprehensive Guide
Step-by-step instructions for your CRISPR knock-in experiments.
Free Download

CRISPR RNA FAQs

How many sgRNA or crRNA sequences are needed for targeted knock-out?

A minimum of 3 crRNA sequences are recommended to ensure knock-out and experimental accuracy. Independently obtained knock-out mutants provide redundancy to safeguard against any hidden off-target effects.
What are the advantages of using synthetic sgRNA for gene editing compared to the traditional plasmids or virus based gRNA delivery methods?
Unlike the traditional plasmids or lentivirus delivery methods, CRISPR/Cas9 RNP are delivered as intact complexes, and do not require cellular expression, thus has many advantages.
- DNA free
- Detectable at high levels shortly after transfection
- Quickly cleared from the cell for less off-target effects
- Highly efficient even in hard-to-transfect cells
- Best for in vivo studies
What is the different between using synthetic sgRNA vs using crRNA: tracrRNA duplex?

When using sgRNA, there is no need for annealing crRNA and tracrRNA duplex prior to use. More importantly, several studies have showed that sgRNA has better stability than crRNA:tracrRNA when duplexed with Cas9, thus leading to higher editing efficiency^1,2.

1. Hendel, et al., Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells. Nat. Biotechnol., 33 (2015) 985-989.
2. Ryan et al., Improving CRISPR–Cas specificity with chemical modifications in single-guide RNAs. Nucleic Acids Research, 46 (2018) 2: 792–803.
What is the advantage of your sgRNAs compared to sgRNA synthesized from in vitro transcription?

Synthesis of 100 nt long sgRNAs was traditionally possible through in vitro-transcription (IVT) using phage RNA polymerase. These in vitro transcribed sgRNAs contain a 5’-triphosphate, which was thought to trigger immune response in many cell types. A recent study showed that sgRNAs with 5’-triphosphate modifications produced through in vitro-transcription can indeed induce innate immune responses and lead to cytotoxicity in human and murine cells. However, chemically synthesized sgRNAs without the 5’-triphosphate modifications demonstrated much better editing efficiency in cells, thus supporting that chemically synthesized sgRNAs are the most ideal reagent for CRISPR genome editing up till now³.

3. Kim et al. CRISPR RNAs trigger innate immune responses in human cells. Genome Res. 2018. 28: 367-373.

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GenCRISPR Synthetic sgRNA

Overview

Advantages