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Home » IVT RNA Production Services » Rush mRNA Synthesis

Overview

As the landscape of mRNA technology rapidly evolves, there is an increased demand for efficient mRNA production. GenScript is proud to offer our customized Rush mRNA Services, designed to expedite your discovery and target screening endeavors, streamline mRNA library construction, and more. Experience our Rush service today, which can save up to 50% of your mRNA project timeline without compromising the quality of the mRNA produced.

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IVT RNA Services

Advantages

As fast as 4 weeks!

As Fast As 2 weeks!

Accelerated screening project
from gene-to-mRNA

Screen multiple targets with acceleration

Reliable mRNA Quality

Proprietary rush mRNA template,
production, and purification process

Reliable Quality

High Protein Expression

Ensuring successful
downstream applications

Specifications

Size/Scale
Options

200bp - 3kb

50 μg

100 μg

Purification

Silica membrane-based method

5' Cap Options

Uncapped

Cap1

Poly(A) Tail Options

100A tail

No tail

Modified NTP Options

N1-Me-Ψ

5moU

Ψ & 5meC

Cy5-UTP or Thio-CTP (after Ψ & 5meC and before Unmodified)

QC

QC Item Test Method Specification Default QC
Identification Appearance Visual inspection Clear and free of foreign particles
RNA Length Agarose gel electrophoresis​ Expected size band detected​
RNA content​ UV absorbance​ Target ± 5%​
pH pH meter Target ± 0.5
Buffer Specification Custom spec N/A
Purity A 260/280 Ratio UV spec 1.70 ~ 2.30

Note: Please contact us for other customized QC such as RNA length, Size-based purity, Endotoxin semi-quantitative etc.

Comparation between Rush mRNA and Standard mRNA

Rush mRNA Standard mRNA
Delivery Time As fast as 2 weeks As fast as 4 weeks
Length Range < 3kb 100 bp – 20 kb
Quantity Range 50 μg
100 μg		 0.1mg - gram level
Design Options
  • Cap1 or Uncapped
  • 100A tail or No tail
  • N1-Me-Ψ / 5moU / Ψ & 5meC /Cy5-UTP or Thio-CTP or Unmodified
  • Cap0, Cap1, Cap2 or Uncapped
  • 100A Tail, Custom tail ≤ 120A or No tail
  • N1-Me-Ψ, 5moU, 5meC & Ψ, Cy5-UTP, Thio-CTP , 5’-Biotin modified, N6-methyl-A, 5-Iodo-C, 5-Iodo-U or Unmodified
QC Appearance, RNA Length, RNA content​, pH, Buffer Specification

Appearance, RNA Length, RNA content​, pH, Buffer Specification

(Additional option: Poly A Length, Capping Efficiency, Size based purity, Total protein residue, Plasmid DNA Residue, dsRNA, Endotoxin, Bioburden)

Case Study

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with GenScript IVT mRNA
Production Services

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