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CRISPR Handbook

CRISPR handbook: Genome Editing manual, free PDF download

The CRISPR Handbook – Enabling Genome Editing and Transforming Life Science Research is GenScript's comprehensive guide to the CRISPR/Cas9 genome editing revolution. This new resource includes:

  • An introductory history of genome editing
  • The current applications for CRISPR/Cas9 in genome editing
  • An updated overview of expanded CRISPR research applications, including immunoprecipitation, epigenetic modification, live imaging, and therapeutics.
  • New CRISPR/Cas9 workflows and case studies to help you start using this technology in your research.

New CRISPR Library workflows to improve the efficacy your screening experiments




New crRNA/Cas9 work studies to optimize your CRISPR editing efficiency.

Figure: CRISPR/Cas9 editing efficiencies for variable Cas9:crRNA concentrations


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CRISPR Services from GenScript

gRNA sequence database

Validated gRNA sequences for efficient, specific targeting of WT SpCas9 or SAM

  gRNA design tool

Developed by Feng Zhang at the Broad Institute, design gRNAs on-demand for any DNA sequence

SAM gRNA constructs

Validated constructs for transcriptional activation

  CRISPR gRNA constructs for genome editing

All-in-one or dual SpCas9, Nickase and SaCas9 constructs for single guide RNA expression

Human SAM libraries

For human genome-wide transcriptional activation,
now in-stock from $4,999

  GeCKO gRNA libraries

For genome wide loss- of-function screening in all-in-one or dual formats, now in stock from $4,999

Microbial gene editing

Efficient KO and KI gene editing in E.coli,
S.cerevisiae and B.subtilis

  CRISPR-edited mammalian cell lines

Fully-validated KO cell lines and cell pools using CRISPR technology

CRISPR Technical Resources

Legal Statement of GenCRISPR Services and Products (Updated on July 28, 2015):

  1. GenCRISPR™ services and products are covered under US 8,697,359, US 8,771,945, US 8,795,965, US 8,865,406, US 8,871,445, US 8,889,356, US 8,889,418, US 8,895,308, US 8,906,616 and foreign equivalents and licensed from Broad Institute, Inc. Cambridge, Massachusetts.
  2. The products and the reagents generated from these services shall be used as tools for research purposes, and shall exclude (a) any human or clinical use, including, without limitation, any administration into humans or any diagnostic or prognostic use, (b) any human germline modification, including modifying the DNA of human embryos or human reproductive cells, (c) any in vivo veterinary or livestock use, or (d) the manufacture, distribution, importation, exportation, transportation, sale, offer for sale, marketing, promotion or other exploitation or use of, or as, a testing service, therapeutic or diagnostic for humans or animals.
  3. The purchase of the GenCRISPR Services and Products coveys to the purchaser the limited, non-transferable right to use the products purchased and the reagents generated from GenCRISPR services and any related material solely for Research Purposes only, not for any Commercial Purposes.

Have questions? GenScript's Ph.D.-level service representatives are available 24 hours a day, Monday through Friday, to assist you.

 
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