GenScript offers two types of library preparation kits, GenTrack2.0 and GenNature2.0, which differ in their specific chemistry. In GenTrack2.0, initial sample preparation or DNA fragmentation, by physical or enzymatic means, is followed by addition of sequencer recognizable components through ligation. In GenNature2.0, sequencer recognizable components are added via transposase enzymes in a tagmentation based method.
GenTrack2.0 offers expanded capabilities including the enzyme based fragmentation module (2.1) and the repairing module (2.2). GenTrack2.0 is more suitable for applications involving shorter DNA products (e.g., PCR products), while GenTrack2.2 may be more appropriate for use with degraded (FFPE) DNA samples. Additionally, GenTrack2.3 includes a UMI module. Overall, the choice between different NGS library preparation kits will depend on the application, sample type, and automation requirements.