Anti-CD Antibodies

Product

GenScript offers both RUO and GMP grade anti-CD antibodies, meeting demands from research through preclinical and clinical phases.

• RUO Grade Anti-CD Antibodies

  Cat. No.	Product Name	Quantity	Price	Order
  A02199	Anti-Human CD3 Antibody (OKT3), mAb, Mouse		$50.00 100 μg
  A02211	Anti-Human CD4 Antibody (F001), mAb, Mouse		$50.00 100 μg
  A02212	Anti-Human CD8 Antibody (F002), mAb, Mouse		$50.00 100 μg
  A02213	Anti-Human CD28 Antibody (F105), mAb, Mouse		$50.00 100 μg

• GMP Grade Anti-CD Antibodies

  Cat. No.	Product Name	Quantity	Price	Order
  GMPA02199	GMP Anti-Human CD3 Antibody (OKT3), mAb, Mouse		$150.00 100 μg 1 mg
  GMPA02213	GMP Anti-Human CD28 Antibody (F105), mAb, Mouse		$150.00 100 μg 1 mg

• New Product Coming Soon

  Cat. No.	Product Name	Order
  GMPA02211	GMP Anti-Human CD4 Antibody (F001), mAb, Mouse	Submit Request
  GMPA02212	GMP Anti-Human CD8 Antibody (F002), mAb, Mouse	Submit Request

*Can’t find the GMP-grade anti-CD antibody you need? Contact us with the RUO-grade catalog number or the CD target! GenScript’s GMP protein platform can fast convert RUO-grade catalog products to GMP-grade.

*GenScript can provide antibody labeling per your request. Examples of labels include PE, FITC, iFluor 488, iFuor 555, iFuor 647, and biotin. Contact us for labeling anti-CD antibodies.

RUO VS. GMP

• Quality Releasing Specifications

  Attributes	GMP* Antibodies	RUO Antibodies
  Concentration	5.0 ± 0.5 mg/mL	1.0 ± 0.1 mg/mL
  Appearance	Clear, colorless liquid	Clear, colorless liquid
  Purity	≥ 95% by SEC-HPLC	≥ 95% by SEC-HPLC
  Endotoxin Level	< 0.1 EU/mg	< 0.5 EU/μg
  Reactivity	Qualified	Qualified
  Identification	100% consistent with the theoretical amino acid sequence by mass spectrometry (MS)	/
  Residual HCP	≤ 100.0 ng/mg	/
  Residual HCD	≤ 10.0 pg/mg	/
  Residual Protein A	≤ 10.0 ng/mg	/
  Sterile	Sterile	/

  *GMP Grade is a specific term that GenScript uses to describe the CD antibodies manufactured in GMP compliant facility and in compliance with guidelines of Current Good Manufacturing Practice (cGMP) with stringent process controls and complete documentation records. GenScript is capable of providing documents, site audits, and other support to help with the applications of your projects in specific regions. Learn More about our GMP capability.

• Features of GMP Grade Anti-CD Antibodies

  Ex vivo Cell Culture Availability	GMP Manufacturing Compliance	High Stability and Lot-to-lot Consistency
  Aseptic verified and animal-free Ultra low endotoxin (< 0.1 EU/mg) HCD, HCP, residual Protein A controlled High purity and high affinity	Traceable documentation GMP guideline compliance Stringent quality control and release Audit and regulatory support	Long-term stability study Accelerated stability study Freeze-thaw cycle stability study Consistent quality among batches

Case Study

• T Cell Activation Using Anti-Human CD3 and Anti-Human CD28 Antibodies

  Anti-CD3 and anti-CD28 antibodies bind to the corresponding CD markers expressed on T cell surface, which provide the first and second signals for T cell activation, respectively. The co-stimulated activating signals induce rapid and robust T cell proliferation, which plays an essential role in adaptive immune response and immunotherapy.

  • T cell Activation Performance using Anti-Human CD3 and Anti-Human CD28 Antibodies
    

    Figure 1. GMP Anti-Human CD3 Antibody (OKT3), mAb, Mouse (Cat. No. GMPA02199) was used for primary T cell activation together with anti-Human CD28 antibody. The percentage of activated T cells were measured by flow cytometry. Data showed that GenScript GMP Anti-Human CD3 Antibody (OKT3), mAb, Mouse outperformed the competing products in activating primary T cells.

  • Use of Anti-Human CD3 and Anti-Human CD28 Antibodies for Studying Signal Response in Reporter Cell Lines
    

    Figure 2. CHO-K1/CD80 cells were plated 15 h before adding Jurkat/IL-2-Luc Promoter cells, then Jurkat/IL-2-Luc Promoter cells were stimulated with a serial of concentrations of Anti-Human CD3 Antibody (OKT3), mAb, Mouse (Cat. No. A02199) for 5 h. Receptor-mediated signaling induced luminescence via activation of the IL-2 pathway.

    Figure 3. Jurkat/IL-2-Luc Promoter cells were co-stimulated with a serial of concentrations of Anti-Human CD28 Antibody (F105), mAb, Mouse (Cat. No. A02213) and 1 μg/mL of Anti-Human CD3 Antibody (OKT3), mAb, Mouse (Cat. No. A02199) for 5 h. Receptor-mediated signaling induced luminescence via activation of the IL-2 pathway.

  • Primary T cell Characterization and Cytokine Secretion Detection after Cell Activation
    

    Figure 4. Primary T cells were stimulated with 5 μg/mL of Anti-Human CD3 Antibody (OKT3), mAb, Mouse (Cat. No. A02199) (left), competing anti-Human CD3 antibody (middle), negative control (NC, right) as well as anti-Human CD28 antibody (all panels) for 72 h, respectively. CD25/CD69 expression was detected by flow cytometry to characterize cell activation.

    

    Figure 5. Primary T cell clumping was observed by a microscope after T cell activation (72 h) (right) using Anti-Human CD3 Antibody (OKT3), mAb, Mouse (Cat. No. A02199) and Anti-Human CD28 Antibody (F105), mAb, Mouse (Cat. No. A02213). The inactivated T cells were presented on the left image.

    

    Figure 6. Interferon γ (IFN-γ) secretion by activated primary T cells using Anti-Human CD3 Antibody (OKT3), mAb, Mouse (Cat. No. A02199) and anti-Human CD28 antibody. Data showed that GenScript anti-Human CD3 antibody outperformed competing product.

    Figure 7. Interferon γ (IFN-γ) secretion by activated primary T cells using Anti-Human CD28 Antibody (F105), mAb, Mouse (Cat. No. A02213) and anti-Human CD3 antibody. Data showed that GenScript anti-Human CD28 antibody outperformed competing product.

• PBMC Staining for T Cell Population Identification Using Anti-Human CD4 and Anti-Human CD8 Antibodies

  Anti-CD antibodies can specifically recognize the CD markers expressed on cells, which can be utilized to identify cells into different cell types or functional cell subsets. e.g., the CD4 and CD8 are the most commonly used markers to identify CD4+ helper T cells and CD8+ cytotoxic T cells in PBMCs staining assays.

  

  Figure 8. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-Human CD4 Antibody (F001), mAb, Mouse (Cat. No. A02211) (left), competing anti-human CD4 antibody (middle), mouse IgG1 negative control (NC, right) as well as with anti-Human CD3 [BV510] antibody (all panels). Flow cytometry was performed for further analysis. Cell debris and dead cells were excluded from the analysis based on scatter signals and 7-AAD.

  

  Figure 9. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-Human CD8 Antibody (F002), mAb, Mouse (Cat. No. A02122) (left), competing anti-Human CD8 antibody (middle), mouse IgG1 negative control (right) as well as with anti-Human CD3 [BV510] antibody (all panels). Flow cytometry was performed for further analysis. Cell debris and dead cells were excluded from the analysis based on scatter signals and 7-AAD.

Product Performance

• High Consistency
  

  Figure 10. Lot-to-lot consistency was tested using four batches of Anti-Human CD3 Antibody (OKT3), mAb, Mouse (Cat. No. A02199) on Jurkat/IL-2-Luc Promoter cells. Receptor-mediated signaling induced luminescence via activation of the IL-2 pathway. Data showed that GenScript Anti-Human CD3 Antibody (OKT3), mAb, Mouse (Cat. No. A02199) has a high lot-to-lot consistency.

  Figure 11. Lot-to-lot consistency was tested using four batches of Anti-Human CD28 Antibody (F105), mAb, Mouse (Cat. No. A02213) on Jurkat/IL-2-Luc Promoter cells. Receptor-mediated signaling induced luminescence via activation of the IL-2 pathway. Data showed that GenScript Anti-Human CD28 Antibody (F105), mAb, Mouse (Cat. No. A02213) has a high lot-to-lot consistency.

• High Stability
  

  Figure 12. Stability of GenScript GMP Anti-Human CD8 Antibody (F002), mAb, Mouse (Cat. No. GMPA02212) was tested. The data of long-term stability, accelerated stability, and freeze-thaw cycle stability indicated that this GMP antibody is very stable under various storage conditions.