Anti-idiotype (anti-Ids) antibodies bind to the idiotype of another antibody or biologic, typically a therapeutic antibody drug. They are used for immunogenicity assays and are widely used to detect and identify antibody drugs in preclinical and clinical studies. They are important reagents for pharmacokinetic (PK) and pharmacodynamic (PD) analysis. GenScript provides a variety of anti-idiotype antibodies to facilitate the development of generic drugs, ADCs, and bispecific antibodies.
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Monoclonal and polyclonal, non-/blocking available
GenScript’s Pembrolizumab Pharmacokinetic ELISA Kit is designed to quantify Pembrolizumab in serum and plasma with high specificity and sensitivity. The precision, accuracy, dilution linearity, specificity, selectivity, stability and hook effect of the kit were evaluated and validated according to ICH M10 and the FDA bioanalytical method validation guidance for industry and are all acceptable.
The kit can be used for the quantification of whole Pembrolizumab and its biosimilars during drug research and development, and also for the detection of single-stranded variable fragments (scFv) of Pembrolizumab.
MRD Analysis of the Detection of Lecanemab in Biological Matrix.
The MRD is the minimum dilution necessary for the detection of Lecanemab in biological
matrix with least interference. Serum samples from cynomolgus monkey were serially diluted
to determine the MRD of this assay, and the test result suggested that MRD was as 1:10.
In this ELISA assay, MonoRab™ Anti-Lecanemab Antibody (44G1), mAb, Rabbit (GenScript,
A02270) was coated at a concentration of 1 μg/ml, and MonoRab™ Anti-Lecanemab Antibody
(4F2), mAb, Rabbit (GenScript, A02271) conjugated with Biotin was used as a detection
antibody at a concentration of 0.5 μg/mL.
Standard Curve of Lecanemab Sandwich ELISA by Anti-Lecanemab
idiotype antibody 44G1 and 4F2.
In this Sandwich ELISA assay, MonoRab™ Anti-Lecanemab Antibody (44G1), mAb, Rabbit
(GenScript, A02270) was coated at a concentration of 1 μg/ml, and MonoRab™
Anti-Lecanemab Antibody (4F2), mAb, Rabbit (GenScript, A02271) conjugated with
Biotin was used as a detection antibody at a concentration of 0.5 μg/mL. GenScript
can provide customized conjugation service for this product per customer's
request.
In this assay, a four-parameter logistic curve fitting program was used to create a
standard curve with the R-Square equal to 0.99971. The typicaldynamic range of the
assay is 0.31 - 20 ng/mL and its detection limit is 0.31 ng/mL.
Standard curve of the detection of Abatacept by Anti-Abatacept
Antibody (CT.E8) and anti-human IgG Fc antibody.
In the ELISA assay shown in this chart, Anti-Abatacept Antibody (CT.E8), mAb, Mouse
(GenScript, A02263) was coated at a concentration of 1 μg/ml, and Mouse Anti-Human
IgG Fc Antibody (50B4A9)[HRP], mAb (GenScript, A01854) was used as a detection
antibody at a dilution of 1:2000.
In this assay, a four-parameter logistic curve fitting program was used to create a
standard curve with the R-Square is greater than 0.99. The typicaldynamic range of
the assay is 4.69-300 ng/mL and its sensitivity of detecting Abatacept is up to 4.69
ng/mL.
Standard curve and specificity analysis of the detection of
Certolizumab pegol by Anti-Certolizumab Antibody (12A3) and anti-PEG
antibody.
In the ELISA assay shown in this chart, THE™ PEG Antibody, mAb, Mouse (GenScript,
A01795) was coated at a concentration of 2 μg/mL, and MonoRab™ Anti-Certolizumab
Antibody (12A3), mAb, Rabbit (GenScript, A02269) conjugated with Biotin was used as
a detection antibody at a concentration of 0.5 μg/mL.
In this assay, a
four-parameter logistic curve fitting program was used to create a standard curve
with the R-Square is greater than 0.999. The typicaldynamic range of the assay is
0.55-400 ng/mL and its sensitivity of detecting Certolizumab pegol is up to 0.55
ng/mL.
Standard curve of the detection of Trastuzumab deruxtecan (DS-8201) by
Anti-Trastuzumab Antibody (15H2) and DXd Antibody (39F4)
In this ELISA assay, Anti-Trastuzumab Antibody (11C4), mAb, Mouse (GenScript, A02032) was
coated at a concentration of 2 μg/mL, and DXd Antibody (39F4), mAb, Mouse (GenScript,
A02217) conjugated with HRP was used as a detection antibody at a concentration of 0.22
μg/mL.
In this assay, a four-parameter logistic curve fitting program was used to create a standard
curve with the R-Square is greater than 0.99. The typicaldynamic range of the assay is 1.56
- 100 ng/mL and its sensitivity of detecting Trastuzumab deruxtecan (DS-8201) is up to 1.56
ng/mL.
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Anti-idiotype Antibodies
Support PK and immunogenicity study of biosimilars