1. Western Blotting
Comparison the sensitivity of DYKDDDDK Tag Antibody from GenScript(A00187, 1 μg/ml) and Sigma (Clone M2, 1 μg/ml) using recombinant proteins with Flag tag at N-terminal, C-terminal Internal positions through Lane1 to Lane3.
Western blot analysis of immunoprecipitates from E.coli lysates containing DYKDDDDDK-tagged protein using THE™ DYKDDDDK Tag Antibody (A00187).
3. Immunofluoenrescent Staining
Immunofluorescence analysis of 293 cells transfected with DYKDDDDK-tagged fusion gene at C-terminal (A)and N-terminal (B) positions using THE™ DYKDDDDK Tag Antibody (A00187).