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GST-tag Antibody [HRP], pAb, Rabbit

This antibody can be used to detect GST-fusion proteins in almost expression systems including bacterial expression system, mammalian expression system and other systems.
A00130
$102.00

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Overview
Specificity This antibody can be used to detect GST-fusion proteins in almost expression systems including bacterial expression system, mammalian expression system and other systems.
Host Species Rabbit
Immunogen Recombinant Glutathione S-Transferase (GST) from Schistosoma japonicum
Species Reactivity Schistosoma japonicum
Conjugate Horseradish peroxidase (HRP)

Applications
Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.05-0.1 µg/ml
Western Blot 0.1-1.0 µg/ml

Properties
Form Lyophilized
Storage Buffer Lyophilized with PBS, pH 7.4, containing 1% BSAand 0.01% Thimerosal
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Storage Instructions The lyophilized product remains stable for up to 1 year at -20 °C from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Purification Affinity chromatography
Isotype Rabbit IgG
Clonality Polyclonal
Clone ID Not applicable

Background
Target Background Glutathione-S-transferase is an epitope tag in generating fusion proteins. Antibodies specific to fusion proteins are useful in monitoring protein expression in Western Blots as well as immunoaffinity purification of proteins expressed in E. coli. The glutathione-S-transferases are a family of enzymes that catalyze the addition of the tripeptide glutathione to endogenous and xenobiotic substrates which have electrophilic functional groups. They play an important role in the detoxication and metabolism of many xenobiotic and endobiotic compounds. The glutathione adducts produced have increased solubility in water and are subsequently enzymatically degraded to mercapturates and excreted.
Synonyms Rabbit Anti-GST-tag [HRP];GST-tag [HRP] Antibody

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


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