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Catalog Products » Antibodies » Epitope Tag Antibodies » Trx-tag Antibody, mAb, Mouse

Trx-tag Antibody, mAb, Mouse

Synonyms Mouse Anti-Trx-tag; Anti-Trx-tag
Description Mouse Anti-Trx-tag Monoclonal Antibody reacts with the 109-aa Trx-tag sequence encoded by the PET-32a expression vectors from Novagen. Mouse Anti-Trx-tag Monoclonal Antibody is suitable for detecting fusion proteins that contain a Trx-tag.
Host Species Mouse
Conjugation Unconjugated
Immunogen Purified recombinant Trx-tag fusion protein
Fusion Partner Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells
Purification Protein A affinity column
Key Features
  • GenScript Trx-tag antibody has high sensitivity.
  • GenScript Trx-tag antibody can recognize N-terminal, C-terminal and internal Trx-tag in fusion proteins.

  • Subclass Mouse IgG2a
    Clone ID 4C12H10
    Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
    Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
    Specificity This antibody reacts with the Thioredoxin fusion protein from E. coli and human. Not yet tested in other species.
    Species Reactivity E. coli and human
    Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

    Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
    ELISA: 0.1-1.0 µg/ml
    Western blot: 1.0 µg/ml
    Western blot using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00205C is recommended. 1.2 µg of this antibody is mixed with diluted WB solution (dilute 2 ml WB with 2ml of PBST).
    Other applications: user-optimized
    Technical Manual ELISA Protocol(pdf)
    FACS Protocol(pdf)
    IHC Protocol(pdf)
    IP Protocol(pdf)


    Cat. No.
    Product Name
    Size
    Price
    Quantity
    A00129-100 Trx-tag Antibody, pAb, Goat
    100 ug
    $199.00
    A00129-40 Trx-tag Antibody, pAb, Goat
    40 ug
    $99.00
    A00160 Goat Anti-Mouse IgG Antibody (H&L) [HRP], pAb
    1 mg
    $79.00
     


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    Cat. No. A00180
    Size
    Price
    40 ug $99.00
    100 ug $199.00
    24 hour
     
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