cGFP-tag Antibody, mAb, Mouse

The antibody reacts with various forms of GFP including eGFP, OFP, and GFPuv.

Price	$102.00
Cat. No.	A00185-40
Size	40 μg Buy in bulk
Quantity
Est. Delivery


Ask us a question

Overview Applications Properties Background Bottom Notes

Overview

Specificity	The antibody reacts with various forms of GFP including eGFP, OFP, and GFPuv.
Host Species	Mouse
Immunogen	Purified recombinant cGFP fusion protein
Conjugate	Unconjugated

Back

Applications

The investigator must determine the ideal working concentration for each specific application.We have not yet determined the suitability of this antibody for applications other than those listed below.The ideal working concentration must take into account such factors as secondary antibody affinity, antigen concentration, sensitivity of the detection method, temperature, and length of incubations.

Application	Recommended Usage
ELISA	0.1-1.0 µg/ml
Western Blot	1.0-2.0 µg/ml
Western Blot	1:100-1:1,000

Back

Properties

Form	Lyophilized
Storage Buffer	Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 1 mg/ml.
Storage Instructions	The lyophilized product remains stable for 1 year at -20℃ from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8℃ or for 12 months at -20℃ or below. Avoid repeated freezing and thawing cycles.
Purification	Protein G affinity column
Isotype	Mouse IgG2b
Clonality	Monoclonal
Clone ID	5E5G11

Back

Background

Target Background	The coral green fluorescent protein (cGFP) from vector pRNATin-H1.2/Neo (GenScript, SD1223) is a versatile marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression. Mouse Anti-cGFP-tag Monoclonal Antibody can be used to detect cGFP fusion protein in Western blot analyses.
Synonyms	Mouse Anti-cGFP-tag mAb;Green Fluorescent Protein

Back

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

Citations

1. Zhang B, et al. Phosphorylation of ATG18a by BAK1 suppresses autophagy and attenuates plant resistance against necrotrophic pathogens. Autophagy. (2020-08)
2. MaLe, et al. Host Factor SPCS1 Regulates the Replication of Japanese Encephalitis Virus through Interactions with Transmembrane Domains of NS2B. J. Virol. (2018)
3. Li Y, et al. Effects of the nuclear localization of the N(pro) protein of classical swine fever virus on its virulence in pigs. Vet Microbiol. (2014-12)
4. Muzammel Haque and Konstantin G. Kousoulas., et al. The Kaposi's Sarcoma-Associated Herpesvirus ORF34 Protein Binds to HIF-1α and Causes Its Degradation via the Proteasome Pathway. J Virol. (2013-02)
5. YanHua Qi, et al. OsARF12, a transcription activator on auxin response gene, regulates root elongation and affects iron accumulation in rice (Oryza sativa). New Phytol. (2012-01)