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Catalog Products » Antibodies » THE™ Elite Antibodies » THE™ HA-tag mAb » cGFP-tag Antibody, mAb, Mouse

cGFP-tag Antibody, mAb, Mouse

CGFP-tag Antibody, MAb, Mouse

Lane 1-3: 100 ng, 25 ng, 10 ng GFP fusion protein
Lane 4: Negative control
Primary antibody:
A. 1 µg/ml Mouse Anti-cGFP-tag Monoclonal Antibody (GenScript, A00185)
B. 1 µg/ml Mouse Anti-cGFP-tag Monoclonal Antibody (X Company)
Secondary antibody: Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:50,000)

CGFP-tag Antibody, MAb, Mouse

Lane 1: OFP transfecting 293 cell lysate
Lane 2: EGFP transfecting 293 cell lysate
Lane 3: 5 ng GFPuv protein
Lane 4: Negative 293 cell lysate
Primary antibody:
A. 1 µg/ml Mouse Anti-cGFP-tag Monoclonal Antibody (GenScript, A00185)
B. 1 µg/ml Mouse Anti-cGFP-tag Monoclonal Antibody (X Company)
Secondary antibody: Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:50,000)

CGFP-tag Antibody, MAb, Mouse

Lane 1-3: 200 ng, 40 ng, 8 ng GFP fusion protein
Detection antibody: Mouse Anti-cGFP-tag Monoclonal Antibody (GenScript, A00185)
The Western blot was performed using One-Step WesternTM Basic Kit (GenScript, L00205)
with 4 µg of the antibody added to 4 ml WB solution.

CGFP-tag Antibody, MAb, Mouse

Specificity analysis of THETM GFP Antibody, mAb, Mouse (GenScript, A00185) by Western blot
using a variety of variants of GFP protein such as eGFP, cGFP and AcGFP1.
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-mouse IgG.

Synonyms Mouse Anti-cGFP-tag mAb; Green Fluorescent Protein
Description The coral green fluorescent protein (cGFP) from vector pRNATin-H1.2/Neo (GenScript, SD1223) is a versatile marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression. Mouse Anti-cGFP-tag Monoclonal Antibody can be used to detect cGFP fusion protein in Western blot analyses.
Host Species Mouse
Conjugation Unconjugated
Immunogen Purified recombinant cGFP fusion protein
Fusion Partner Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells.
Purification Protein G affinity column
Key Features
  • GenScript cGFP-tag antibody has high sensitivity.
  • GenScript cGFP-tag antibody can recognize various forms of GFP proteins including eGFP, OFP and GFPuv.

  • Subclass Mouse IgG2b
    Clone ID 5E5G11
    Concentration 1 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
    Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 1 mg/ml.
    Specificity The antibody reacts with various forms of GFP including eGFP, OFP, and GFPuv.
    Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

    The investigator must determine the ideal working concentration for each specific application. We have not yet determined the suitability of this antibody for applications other than those listed below. The ideal working concentration must take into account such factors as secondary antibody affinity, antigen concentration, sensitivity of the detection method, temperature, and length of incubations
    ELISA: 0.1-1.0 µg/ml
    Western blot: 1.0-2.0 µg/ml
    Western blot using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00205C is recommended. 4 µg of this antibody is mixed with 4 ml of WB solution.
    Other applications: user-optimized
    Technical Manual ELISA Protocol(pdf)
    Western Blot Protocol(pdf)
    FACS Protocol(pdf)
    IHC Protocol(pdf)
    IP Protocol(pdf)

    CGFP-tag Antibody, MAb, Mouse
    CGFP-tag Antibody, MAb, Mouse

    Lane 1-3: 100 ng, 25 ng, 10 ng GFP fusion protein
    Lane 4: Negative control
    Primary antibody:
    A. 1 µg/ml Mouse Anti-cGFP-tag Monoclonal Antibody (GenScript, A00185)
    B. 1 µg/ml Mouse Anti-cGFP-tag Monoclonal Antibody (X Company)
    Secondary antibody: Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:50,000)

    CGFP-tag Antibody, MAb, Mouse
    CGFP-tag Antibody, MAb, Mouse

    Lane 1: OFP transfecting 293 cell lysate
    Lane 2: EGFP transfecting 293 cell lysate
    Lane 3: 5 ng GFPuv protein
    Lane 4: Negative 293 cell lysate
    Primary antibody:
    A. 1 µg/ml Mouse Anti-cGFP-tag Monoclonal Antibody (GenScript, A00185)
    B. 1 µg/ml Mouse Anti-cGFP-tag Monoclonal Antibody (X Company)
    Secondary antibody: Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:50,000)

    CGFP-tag Antibody, MAb, Mouse
    CGFP-tag Antibody, MAb, Mouse

    Lane 1-3: 200 ng, 40 ng, 8 ng GFP fusion protein
    Detection antibody: Mouse Anti-cGFP-tag Monoclonal Antibody (GenScript, A00185)
    The Western blot was performed using One-Step WesternTM Basic Kit (GenScript, L00205)
    with 4 µg of the antibody added to 4 ml WB solution.

    CGFP-tag Antibody, MAb, Mouse
    CGFP-tag Antibody, MAb, Mouse

    Specificity analysis of THETM GFP Antibody, mAb, Mouse (GenScript, A00185) by Western blot
    using a variety of variants of GFP protein such as eGFP, cGFP and AcGFP1.
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-mouse IgG.


    Muzammel Haque and Konstantin G. Kousoulas. The Kaposi's Sarcoma-Associated Herpesvirus ORF34 Protein Binds to HIF-1α and Causes Its Degradation via the Proteasome Pathway. J Virol. 2013 Feb;87(4):2164 - 2173.

    Y Liu.,et al. Characterization Of The Open Reading Frame 7A From Bombyx Mori Nucleopolyhedrovirus.Mol Biol Rep.2013Feb;

    YanHua Qi.,et al. OsARF12, a transcription activator on auxin response gene, regulates root elongation and affects iron accumulation in rice (Oryza sativa).New Phytol.2012Jan;

    Jeremy E. Wilusz.,et al. A Triple Helix Stabilizes The 3' Ends Of Long Noncoding Rnas That Lack Poly(A) Tails.Genes Dev.2012Nov;26(21):2392 - 2407

    W.-X. Liu.,et al. Inhibition effect produced by dominant negative mutant fusion protein PreS2‐TLM‐ScFv‐HBcDN on HBV replication in vitro.J Viral Hepat.2012Apr;19(4):295-300

    Yanjun Yang.,et al. A chloroplast envelope membrane protein containing a putative LrgB domain related to the control of bacterial death and lysis is required for chloroplast development in Arabidopsis thaliana.New Phytol.2012Jan;193(1):81-95

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    For more documents, please visit "Technical Support".

    Cat. No. A00185
    Size
    Price
    40 ug $99.00
    200 ug $349.00
    100 ug $199.00
    24 hour
     
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