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Support » Promotions » Product Promotions » Free Samples » THETM His Tag Antibody, mAb, Mouse

THE™ His Tag Antibody, mAb, Mouse

THE™ His Tag Antibody, MAb, Mouse

Western blot analysis of His-tagged fusion proteins using THETM His Antibody, mAb, Mouse (GenScript, A00186, 1 µg/ml)
Lane 1: N-terminal His-tagged fusion protein
Lane 2: C-terminal His-tagged fusion protein
The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221).

THE™ His Tag Antibody, MAb, Mouse

Western blot analysis of Multiple Tag Cell Lysate (GenScript, M0100) using THETM His Antibody, mAb, Mouse (GenScript, A00186, 1 µg/ml)
The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221).
Predicted Size: 52 kD
Observed Size: 52 kD

THE™ His Tag Antibody, MAb, Mouse

Comparison of THETM His Antibody, mAb, Mouse (A: GenScript, A00186, 1 µg/ml) with Mouse Anti-His mAbs (B: Competitor Q#1, 1 µg/ml; C: Competitor Q#2, 1 µg/ml) by Dot blot.
The assay was performed with His-tagged fusion protein.
The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221).

THE™ His Tag Antibody, MAb, Mouse

Comparison of THETM His Antibody, mAb, Mouse (A: GenScript, A00186, 0.1 µg/ml) with Mouse Anti-His mAb (B: Competitor A, 0.1 µg/ml) by Western blot.
The assay was performed with His-tagged fusion protein.
The signal was developed with One-Step WesternTM Basic Kit (GenScript, L00205).

THE™ His Tag Antibody, MAb, Mouse

Comparison of THETM His Antibody, mAb, Mouse (A: GenScript, A00186, 1 µg/ml) with Mouse Anti-His mAb (B: Competitor B, 1 µg/ml) by Western blot.
The assay was performed with cell lysates containing His-tagged fusion protein.
The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221).

THE™ His Tag Antibody, MAb, Mouse

Consistency analysis of Batch 1#, 2#, 3# and 4# of THETM His Antibody, mAb, Mouse (GenScript, A00186, 1 µg/ml) by Western blot, showing that signal remains consistent from Lot to Lot.
The assay was performed with His-tagged fusion protein.
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Mouse IgG.

THE™ His Tag Antibody, MAb, Mouse

Western blot analysis of immunoprecipitates from cell lysates containing His fusion protein using THETM His Antibody, mAb, Mouse (GenScript, A00186).
1. Positive control containg His fusion protein
2. Negative control – IP with isotype control antibody (A01007)
3. Immunoprecipitation with THETM His Tag Antibody, mAb, Mouse (A00186)

Specificity THETM His Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
Host Species Mouse
Immunogen A synthetic peptide HHHHHH coupled to KLH

Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Purification Protein A affinity column
Clone ID 6G2A9

  • THE™ His Tag Antibody, MAb, Mouse
  • THE™ His Tag Antibody, MAb, Mouse

    Western blot analysis of His-tagged fusion proteins using THETM His Antibody, mAb, Mouse (GenScript, A00186, 1 µg/ml)
    Lane 1: N-terminal His-tagged fusion protein
    Lane 2: C-terminal His-tagged fusion protein
    The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221).

  • THE™ His Tag Antibody, MAb, Mouse
  • THE™ His Tag Antibody, MAb, Mouse

    Western blot analysis of Multiple Tag Cell Lysate (GenScript, M0100) using THETM His Antibody, mAb, Mouse (GenScript, A00186, 1 µg/ml)
    The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221).
    Predicted Size: 52 kD
    Observed Size: 52 kD

  • THE™ His Tag Antibody, MAb, Mouse
  • THE™ His Tag Antibody, MAb, Mouse

    Comparison of THETM His Antibody, mAb, Mouse (A: GenScript, A00186, 1 µg/ml) with Mouse Anti-His mAbs (B: Competitor Q#1, 1 µg/ml; C: Competitor Q#2, 1 µg/ml) by Dot blot.
    The assay was performed with His-tagged fusion protein.
    The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221).

  • THE™ His Tag Antibody, MAb, Mouse
  • THE™ His Tag Antibody, MAb, Mouse

    Comparison of THETM His Antibody, mAb, Mouse (A: GenScript, A00186, 0.1 µg/ml) with Mouse Anti-His mAb (B: Competitor A, 0.1 µg/ml) by Western blot.
    The assay was performed with His-tagged fusion protein.
    The signal was developed with One-Step WesternTM Basic Kit (GenScript, L00205).

  • THE™ His Tag Antibody, MAb, Mouse
  • THE™ His Tag Antibody, MAb, Mouse

    Comparison of THETM His Antibody, mAb, Mouse (A: GenScript, A00186, 1 µg/ml) with Mouse Anti-His mAb (B: Competitor B, 1 µg/ml) by Western blot.
    The assay was performed with cell lysates containing His-tagged fusion protein.
    The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221).

  • THE™ His Tag Antibody, MAb, Mouse
  • THE™ His Tag Antibody, MAb, Mouse

    Consistency analysis of Batch 1#, 2#, 3# and 4# of THETM His Antibody, mAb, Mouse (GenScript, A00186, 1 µg/ml) by Western blot, showing that signal remains consistent from Lot to Lot.
    The assay was performed with His-tagged fusion protein.
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Mouse IgG.

  • THE™ His Tag Antibody, MAb, Mouse
  • THE™ His Tag Antibody, MAb, Mouse

    Western blot analysis of immunoprecipitates from cell lysates containing His fusion protein using THETM His Antibody, mAb, Mouse (GenScript, A00186).
    1. Positive control containg His fusion protein
    2. Negative control – IP with isotype control antibody (A01007)
    3. Immunoprecipitation with THETM His Tag Antibody, mAb, Mouse (A00186)


Target Background Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells. However, since 6XHis-tag is poorly immunogenic, it needs to be conjugated to KLH or some other carrier as an immunogen. After hundreds of selection cycles, researchers at GenScript successfully isolated an antibody against His-tag.
THETM His Tag Antibody, mAb, Mouse (subtype IgG1) has very high affinity. Tests performed at GenScript show that the antibody can also recognize 4xHis- and 5xHis-tags. This means that even if the 6xHis-tag is only partially exposed, it will still be recognized and bound by this antibody.
THETM His Tag mAb is produced from mice ascites and purified by protein A affinity column. This antibody recognizes native as well as denatured forms of synthetic polyhistidine and polyhistidine-tagged fusion proteins. The product reacts with fusion proteins expressed in bacteria, insect cells, and mammalian cells. THETM His Tag mAb recognizes His tags placed at N-terminal, C-terminal, and internal regions of fusion proteins.
THETM His Tag mAb can be used in Western blot analyses, Dot blot analyses, ELISA, immunofluorescent staining, and flow cytometry of cultured cells.
Synonyms THETM Anti-His mAb;

Cat. No. Product Name Quantity Price
A01620
THE™ His Tag Antibody [FITC], mAb, Mouse - 100 μg
$410.00
A00612
THE™ His Tag Antibody [HRP], mAb, Mouse - 100 μg
$410.00
A01800-100
THE™ His Tag Antibody [iFluor 488], mAb, Mouse - 100 μg
$450.00
A01801-100
THE™ His Tag Antibody [iFluor 555], mAb, Mouse - 100 μg
$450.00
A01802-100
THE™ His Tag Antibody [iFluor 647], mAb, Mouse - 100 μg
$450.00
A00613
THE™ His Tag Antibody [Biotin], mAb, Mouse - 100 μg
$410.00
A00205-40
His-tag antibody [Biotin], pAb, Rabbit - 40 μg
$99.00
 


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Cat. No. A00186S
Size Price
10 μg $0.00