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Support » Promotions » Product Promotions » Free Samples » THETM DYKDDDDK Tag Antibody, mAb, Mouse

THE™ DYKDDDDK Tag Antibody, mAb, Mouse

THE™ DYKDDDDK Tag Antibody, MAb, Mouse

Flow cytometric analysis of non-transfected or Flag fusion gene transfected CHO cells using
THETM DYKDDDDK Tag Antibody, mAb, Mouse (GenScript, A00187) (black and yellow respectively). The signal was developed with FITC conjugated Goat Anti-Mouse IgG.

Host Species Mouse
Conjugation Unconjugated
Immunogen A synthetic peptide (DYKDDDDK) coupled to KLH
Fusion Partner Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells.
Purification Protein A affinity column

Subclass IgG2b, k
Clone ID 5A8E5
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Immunoprecipitation: 1 µg/ml
Immunofluorescent staining: 1 µg/ml
Flow cytometry: 1 µg/ml
Other applications: user-optimized

  • THE™ DYKDDDDK Tag Antibody, MAb, Mouse
  • THE™ DYKDDDDK Tag Antibody, MAb, Mouse

    Flow cytometric analysis of non-transfected or Flag fusion gene transfected CHO cells using
    THETM DYKDDDDK Tag Antibody, mAb, Mouse (GenScript, A00187) (black and yellow respectively). The signal was developed with FITC conjugated Goat Anti-Mouse IgG.


Synonyms Mouse anti DYKDDDDK-tag mAb; Mouse anti flag-tag mAb;
Target Background The DYKDDDDK peptide is a small component of the epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. THETM DYKDDDDK Tag Antibody, mAb, Mouse is a high-affinity monoclonal antibody that can be used to detect DYKDDDDK-tagged proteins.

Cat. No. Product Name Quantity Price
A01429-100
THE™ DYKDDDDK Tag Antibody [Biotin], mAb, Mouse - 100 μg
$149.00
A01428-100
THE™ DYKDDDDK Tag Antibody [HRP], mAb, Mouse - 100 μg
$149.00
 


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Cat. No. A00187S
Size Price
10 μg $0.00