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THE™ DYKDDDDK Tag Antibody, mAb, Mouse

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Flow cytometric analysis of non-transfected or Flag fusion gene transfected CHO cells using
THE^TM DYKDDDDK Tag Antibody, mAb, Mouse (GenScript, A00187) (black and yellow respectively). The signal was developed with FITC conjugated Goat Anti-Mouse IgG.

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Overview

Host Species	Mouse
Conjugation	Unconjugated
Immunogen	A synthetic peptide (DYKDDDDK) coupled to KLH
Fusion Partner	Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells.
Purification	Protein A affinity column

Properties

Subclass	IgG2b, k
Clone ID	5A8E5
Concentration	0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution	Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Immunoprecipitation: 1 µg/ml
Immunofluorescent staining: 1 µg/ml
Flow cytometry: 1 µg/ml
Other applications: user-optimized

Examples

Background

Synonyms	Mouse anti DYKDDDDK-tag mAb; Mouse anti flag-tag mAb;
Target Background	The DYKDDDDK peptide is a small component of the epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. THE^TM DYKDDDDK Tag Antibody, mAb, Mouse is a high-affinity monoclonal antibody that can be used to detect DYKDDDDK-tagged proteins.