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Catalog Products » Antibodies » Primary Antibodies » GPCR Antibodies » GAPDH Antibody [HRP], pAb, Goat

GAPDH Antibody [HRP], pAb, Goat

GAPDH Antibody [HRP], PAb, Goat

Western blot analysis of cell and tissue lysates using GAPDH Antibody [HRP], pAb, Goat (GenScript, A00192, 0.2 µg/ml)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 36 kD
Observed Size: 36 kD

GAPDH Antibody [HRP], PAb, Goat

Western blot analysis of cell lysates using GAPDH Antibody [HRP], pAb, Goat (GenScript, A00192, 1 µg/ml)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 36 kD
Observed Size: 36 kD

GAPDH Antibody [HRP], PAb, Goat

Western blot analysis of cell lysates and tissue lysates using GAPDH Antibody [HRP], pAb, Goat (GenScript, A00192, 0.05 µg/ml)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 36 kD
Observed Size: 36 kD

Synonyms Anti-GAPDH [HRP];
Description Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is one of the key enzymes involved in glycolysis. Though expressed differently in different tissues, GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH is often measured as a control in experiments quantifying specific changes in expression of other targets. Recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. GAPDH may also play a role in neurodegenerative pathologies such as Huntingtons and Alzheimers disease. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
Host Species Goat
Conjugation Peroxidase (Horseradish)
Immunogen Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from rabbit muscle
Purification Immunoaffinity chromatography

Subclass Goat IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH7.4, containing 1%BSA, 0.01% thimerosal
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to the final concentration of 0.5mg/ml.
Specificity GenScript Goat Anti-GAPDH [HRP] Polyclonal Antibody specifically reacts with GAPDH from human, mouse, rat, goat, rabbit, chicken, hamster, bovine, swine, fish, and E. coli cell and tissue lysates. It has not yet been tested with other species.
Species Reactivity Human, mouse, rat, goat, rabbit, chicken, hamster, bovine, swine, fish, and E. coli
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized

  • GAPDH Antibody [HRP], PAb, Goat
  • GAPDH Antibody [HRP], PAb, Goat

    Western blot analysis of cell and tissue lysates using GAPDH Antibody [HRP], pAb, Goat (GenScript, A00192, 0.2 µg/ml)
    The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221)
    Predicted Size: 36 kD
    Observed Size: 36 kD

  • GAPDH Antibody [HRP], PAb, Goat
  • GAPDH Antibody [HRP], PAb, Goat

    Western blot analysis of cell lysates using GAPDH Antibody [HRP], pAb, Goat (GenScript, A00192, 1 µg/ml)
    The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221)
    Predicted Size: 36 kD
    Observed Size: 36 kD

  • GAPDH Antibody [HRP], PAb, Goat
  • GAPDH Antibody [HRP], PAb, Goat

    Western blot analysis of cell lysates and tissue lysates using GAPDH Antibody [HRP], pAb, Goat (GenScript, A00192, 0.05 µg/ml)
    The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221)
    Predicted Size: 36 kD
    Observed Size: 36 kD


Cat. No.
Product Name
Price
A00915-40
GAPDH Antibody [Biotin], pAb, Goat - 40 ug
$99.00
A00191-40
GAPDH Antibody, pAb, Goat - 40 ug
$99.00
 



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Cat. No. A00192
Size
Price
40 ug $99.00