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Catalog Products » Antibodies » Epitope Tag Antibodies » VSV-G-tag Antibody, pAb, Rabbit

VSV-G-tag Antibody, pAb, Rabbit

VSV-G-tag Antibody, PAb, Rabbit

Western blot analysis of VSV-G-tagged fusion protein using VSV-G-tag Antibody, pAb, Rabbit (GenScript, A00199, 0.1 µg/ml)
The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 52 kD
Observed Size: 52 kD

VSV-G-tag Antibody, PAb, Rabbit

Western blot analysis of VSV-G-tagged fusion protein using VSV-G-tag Antibody, pAb, Rabbit (GenScript, A00199, 1 µg/ml)
The signal was developed with One-Step WesternTM Basic Kit (Rabbit) (GenScript, L00204)
Predicted Size: 52 kD
Observed Size: 52 kD

VSV-G-tag Antibody, PAb, Rabbit

Western blot analysis of immunoprecipitates from VSV-G fusion protein lysates using Rabbit Anti-VSV-G-tag Polyclonal Antibody (GenScript, A00199).
Lane 1: Input control material for VSV-G fusion protein lysates
Lane 2: Negative control – IP with isotype control antibody (GenScript, A01008)
Lane 3: Immunprecipitation with Rabbit Anti-VSV-G-tag Polyclonal Antibody (GenScript, A00199).

VSV-G-tag Antibody, PAb, Rabbit

Western blot analysis of VSV-G tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-VSV-G-tag Polyclonal Antibody (GenScript, A00199, 1 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

Synonyms Anti-VSV-G-tag;
Description Rabbit Anti-VSV-G-tag Polyclonal Antibody is supplied as a 40 μg aliquot at a concentration of 1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide. It is purified by immunoaffinity chromatography.
Host Species Rabbit
Conjugation Unconjugated
Immunogen VSV-G epitope tag peptide YTDIEMNRLGK conjugated to KLH
Purification Immunoaffinity chromatography

Subclass IgG
Concentration 1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide
Specificity This Antibody recognizes VSV-G tagged fusion proteins.
Storage The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western Blot Using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Immunoprecipitation (IP): 2-10 µg/mg of lysate
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)
IF & ICC Protocol(pdf)

VSV-G-tag Antibody, PAb, Rabbit
VSV-G-tag Antibody, PAb, Rabbit

Western blot analysis of VSV-G-tagged fusion protein using VSV-G-tag Antibody, pAb, Rabbit (GenScript, A00199, 0.1 µg/ml)
The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 52 kD
Observed Size: 52 kD

VSV-G-tag Antibody, PAb, Rabbit
VSV-G-tag Antibody, PAb, Rabbit

Western blot analysis of VSV-G-tagged fusion protein using VSV-G-tag Antibody, pAb, Rabbit (GenScript, A00199, 1 µg/ml)
The signal was developed with One-Step WesternTM Basic Kit (Rabbit) (GenScript, L00204)
Predicted Size: 52 kD
Observed Size: 52 kD

VSV-G-tag Antibody, PAb, Rabbit
VSV-G-tag Antibody, PAb, Rabbit

Western blot analysis of immunoprecipitates from VSV-G fusion protein lysates using Rabbit Anti-VSV-G-tag Polyclonal Antibody (GenScript, A00199).
Lane 1: Input control material for VSV-G fusion protein lysates
Lane 2: Negative control – IP with isotype control antibody (GenScript, A01008)
Lane 3: Immunprecipitation with Rabbit Anti-VSV-G-tag Polyclonal Antibody (GenScript, A00199).

VSV-G-tag Antibody, PAb, Rabbit
VSV-G-tag Antibody, PAb, Rabbit

Western blot analysis of VSV-G tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-VSV-G-tag Polyclonal Antibody (GenScript, A00199, 1 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.


Cat. No.
Product Name
Size
Price
Quantity
A00098 Goat Anti-Rabbit IgG Antibody (H&L) [HRP], pAb
1 mg
$79.00
A00872-40 VSV-G-tag Antibody [HRP], pAb, Rabbit
40 ug
$99.00
 
S Schmid.,et al. MAP Kinase-Mediated Licensing of Interferon Regulatory Factor (IRF) 3/7 Reinforces the Cell Response to Virus.J Biol Chem.2013Nov;

Zhang XM.,et al. In vitro inhibition of vesicular stomatitis virus replication by purified porcine Mx1 protein fused to HIV-1 Tat protein transduction domain (PTD).Antiviral Res.2013Aug;99(2):149-57

Elizabeth M. Kita.,et al. Neuropilin-1 biases dendrite polarization in the retina.Development.2013Jun;140:2933 - 2941

Zhao SL.,et al. Surface Display Of Rice Stripe Virus Nsvc2 And Analysis Of Its Membrane Fusion Activity.Virol Sin.2012Apr;27(2):100 - 8

Padmashali RM.,et al. Engineering fibrinogen-binding VSV-G envelope for spatially-and cell-controlled lentivirus delivery through fibrin hydrogels.Biomaterials.2011Apr;32(12):3330-9

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For more documents, please visit "Technical Support".

Cat. No. A00199
Size
Price
40 ug $99.00
 
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