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Catalog Products » Antibodies » Epitope Tag Antibodies » GST-tag Antibody [Biotin], pAb, Rabbit

GST-tag Antibody [Biotin], pAb, Rabbit

Synonyms Anti-GST-tag [Biotin];
Description Rabbit Anti-GST-tag [Biotin] Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to biotin.
Host Species Rabbit
Antigen Species Schistosoma japonicum
Conjugation Biotin
Immunogen Recombinant Glutathione S-Transferase (GST) from Schistosoma japonicum
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 1 mg/ml in PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide
Specificity This antibody is specific for the GST protein from Schistosoma japonicum and is useful for detecting GST and GST-fusion proteins. Cross-reactivity with cell lysate of E. coli bacteria, sf21 cell and yeast have not been observed.
Species Reactivity Schistosoma japonicum
Storage The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.01-0.1 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

GST-tag Antibody [Biotin], PAb, Rabbit
GST-tag Antibody [Biotin], PAb, Rabbit

Western blot analysis of GST-tagged fusion protein using GST-tag Antibody [Biotin], pAb, Rabbit (GenScript, A00202, 1 µg/ml)
The signal was developed with Streptavidin-HRP (GenScript, M00091, 1:5,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 31 kD
Observed Size: 31 kD, 62 kD


Cat. No.
Product Name
Size
Price
Quantity
A00130-100 GST-tag Antibody [HRP], pAb, Rabbit
100 ug
$199.00
A00130-40 GST-tag Antibody [HRP], pAb, Rabbit
40 ug
$99.00
A00202-100 GST-tag Antibody [Biotin], pAb, Rabbit
100 ug
$199.00
A00202-40 GST-tag Antibody [Biotin], pAb, Rabbit
40 ug
$99.00
A00865-100 THETM GST Antibody, mAb, Mouse
100 ug
$175.00
A00865-200 THETM GST Antibody, mAb, Mouse
200 ug
$311.00
A00867-40 THETM GST Antibody [Biotin], mAb, Mouse
40 ug
$74.00
A01380-40 GST-tag Antibody [HRP], pAb, Goat
40 ug
$99.00
L00207 GST Fusion Protein Purification Kit
1 kit
$139.00
L00411 GST tag ELISA Detection Kit
1 kit (1x96 well)
$298.00
A00866-100 THETM GST Antibody [HRP], mAb, Mouse
100 ug
$180.00
A00097-100 GST-tag Antibody, pAb, Rabbit
100 ug
$199.00
A01380-100 GST-tag Antibody [HRP], pAb, Goat
100 ug
$199.00
 


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Cat. No. A00202
Size
Price
40 ug $99.00
100 ug $199.00
24 hour
 
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