His-tag antibody [Biotin], pAb, Rabbit Antibody

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Overview

Synonyms	His-tag antibody [Biotin], pAb, Rabbit;
Description	GenScript His-tag antibody [Biotin], pAb, Rabbit is the biotin conjugated Rabbit anti His antibody. The antibody recognizes fusion protein with His tag which is located at either C-terminal, or internal regions of the protein. It is suitable for detecting the expression level of His tagged proteins. GenScript His-tag antibody [Biotin], pAb, Rabbit is the biotin conjugated Rabbit anti His antibody. It is suitable for detecting the expression level of His tagged proteins.
Host Species	Rabbit
Conjugation	Biotin
Immunogen	His epitope tag peptide HHHHHH conjugated to KLH.
Purification	Immunoaffinity chromatography

Properties

Subclass	IgG
Concentration	0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity	GenScript His-tag antibody [Biotin], pAb, Rabbit specifically reacts with fusion proteins containing His tagged fusion proteins.
Storage	The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.2-1 µg/ml
Other Applications: user optimized

Related Products

Cat. No.	Product Name	Price
L00440w	His Tag Antibody Plate (white, 96 wells, nonremovable) - 5 plates	$179.00
L00440c	His Tag Antibody Plate (clear, 8x12 strips) - 5 plates	$179.00
L00440b	His Tag Antibody Plate (black, 96 wells, nonremovable) - 5 plates	$179.00
L00439-1	Anti-His Affinity Resin - 1 ml	$399.00
L00439-5	Anti-His Affinity Resin - 5 ml	$1599.00
L00436	His Tag ELISA Detection Kit - 1 kit	$298.00
A01802-100	THE^TM His Tag Antibody [iFluor 647], mAb, Mouse - 100 ug	$450.00
A01801-100	THE^TM His Tag Antibody [iFluor 555], mAb, Mouse - 100 ug	$450.00
A01800-100	THE^TM His Tag Antibody [iFluor 488], mAb, Mouse - 100 ug	$450.00
A01620	THE^TM His Tag Antibody [FITC], mAb, Mouse - 100 ug	$410.00
A00613	THE^TM His Tag Antibody [Biotin], mAb, Mouse - 100 ug	$410.00
A00612	THE^TM His Tag Antibody [HRP], mAb, Mouse - 100 ug	$410.00
A00205-40	His-tag antibody [Biotin], pAb, Rabbit - 40 ug	$99.00
A00186-100	THE^TM His Tag Antibody, mAb, Mouse - 100 ug	$375.00
A00174-40	His-tag Antibody, pAb, Rabbit - 40 ug	$99.00

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Sicong Dong and Gary W. Blissard..,et al. Functional Analysis Of The Autographa Californica Multiple Nucleopolyhedrovirus Gp64 Terminal Fusion Loops And Interactions With Membranes.J Virol.2012Sep;86(18):9617 - 9628

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