Western blot analysis of His tagged fusion protein (Genscript, M0100) using THETM His Tag Antibody [Biotin], mAb, mouse (GenScript, A00613, 1 µg/ml) The signal was developed with Streptavidin-HRP (GenScript, M00091, 1:10,000) and and LumiSensorTM HRP Substrate Kit (GenScript, L00221) Predicted Size: 52 KD Observed Size: 52 KD
Working concentrations for specific applications should be determined by the investigator.Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.
Lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide
Reconstitution
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Storage Instructions
The antibody is stable in lyophilized form if stored at -20°C or below.The reconstituted antibody can be stored for 2-3 weeks at 2-8°C.For long term storage, aliquot and store at -20°C or below.Avoid repeated freezing and thawing cycles.
Western blot analysis of His tagged fusion protein (Genscript, M0100) using THETM His Tag Antibody [Biotin], mAb, mouse (GenScript, A00613, 1 µg/ml) The signal was developed with Streptavidin-HRP (GenScript, M00091, 1:10,000) and and LumiSensorTM HRP Substrate Kit (GenScript, L00221) Predicted Size: 52 KD Observed Size: 52 KD
GenScript THE™ His Tag Antibody [Biotin], mAb, mouse is the biotin conjugated THE™ His Tag Antibody, mAb, mouse (A00186) purified from mice ascites by protein A affinity column.The antibody is suitable for detecting the expression level of His in fusion proteins.
Synonyms
THE™ His Tag Antibody [Biotin], mAb, Mouse;
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.