THE™ His Tag Antibody [Biotin], mAb, Mouse
![THE™ His Tag Antibody [Biotin], MAb, Mouse](/product/documents/down?doc_name=3376_20070711050658.JPG&file=filecenter/document/3376_20070711050658.JPG "THE™ His Tag Antibody [Biotin], MAb, Mouse")
Western blot analysis of His tagged fusion protein (Genscript, M0100) using THETM His Tag Antibody [Biotin], mAb, mouse (GenScript, A00613, 1 µg/ml)
The signal was developed with Streptavidin-HRP (GenScript, M00091, 1:10,000) and and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 52 KD
Observed Size: 52 KD
| Specificity | GenScript THE™ His Tag Antibody [Biotin], mAb, Mouse specificly recognizes C-terminal, N-terminal, and internal His tagged fusion proteins. |
| Host Species | Mouse |
| Immunogen | A synthetic peptide (HHHHHH) coupled to KLH |
| Conjugate | Biotin |
| Working concentrations for specific applications should be determined by the investigator.Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product. | |||||||||
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| Form | Lyophilized |
| Storage Buffer | Lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide |
| Reconstitution | Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml. |
| Storage Instructions | The antibody is stable in lyophilized form if stored at -20°C or below.The reconstituted antibody can be stored for 2-3 weeks at 2-8°C.For long term storage, aliquot and store at -20°C or below.Avoid repeated freezing and thawing cycles. |
| Purification | Protein A affinity column |
| Isotype | Mouse IgG1,κ |
| Clonality | Monoclonal |
| Clone ID | 6G2A9 |
Western blot analysis of His tagged fusion protein (Genscript, M0100) using THETM His Tag Antibody [Biotin], mAb, mouse (GenScript, A00613, 1 µg/ml)
The signal was developed with Streptavidin-HRP (GenScript, M00091, 1:10,000) and and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 52 KD
Observed Size: 52 KD
| Target Background | GenScript THE™ His Tag Antibody [Biotin], mAb, mouse is the biotin conjugated THE™ His Tag Antibody, mAb, mouse (A00186) purified from mice ascites by protein A affinity column.The antibody is suitable for detecting the expression level of His in fusion proteins. |
| Synonyms | THE™ His Tag Antibody [Biotin], mAb, Mouse; |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
Yongbing Pan, et al. Screening of potent neutralizing antibodies against SARS-CoV-2 using convalescent patients-derived phage-display libraries. Cell Discov. (2021-07)
Temming AR, et al. Cross-reactivity of mouse IgG subclasses to human Fc gamma receptors: Antibody deglycosylation only eliminates IgG2b binding. Molecular immunology. (2020-11)
HussainKhiyam, et al. Impact of Human FcγR Gene Polymorphisms on IgG-Triggered Cytokine Release: Critical Importance of Cell Assay Format. Front Immunol. (2019-01)
QiXinyue, et al. Optimization of 4-1BB antibody for cancer immunotherapy by balancing agonistic strength with FcγR affinity. Nat Commun. (2019)
Sow HS, et al. FcγR interaction is not required for effective anti-PD-L1 immunotherapy but can add additional benefit depending on the tumor model. Int J Cancer. (2019)
V Hernandez-Gordillo, et al. Mimicking the extracellular matrix with functionalized, metal-assembled collagen peptide scaffolds. Biomaterials. (2014-08)
Fiona Wegman, et al. Gene delivery of bone morphogenetic protein-2 plasmid DNA promotes bone formation in a large animal model. J Tissue Eng Regen Med. (2012-08)
You can visit "Citations Database" for more citations.
Temming AR, et al. Cross-reactivity of mouse IgG subclasses to human Fc gamma receptors: Antibody deglycosylation only eliminates IgG2b binding. Molecular immunology. (2020-11)
HussainKhiyam, et al. Impact of Human FcγR Gene Polymorphisms on IgG-Triggered Cytokine Release: Critical Importance of Cell Assay Format. Front Immunol. (2019-01)
QiXinyue, et al. Optimization of 4-1BB antibody for cancer immunotherapy by balancing agonistic strength with FcγR affinity. Nat Commun. (2019)
Sow HS, et al. FcγR interaction is not required for effective anti-PD-L1 immunotherapy but can add additional benefit depending on the tumor model. Int J Cancer. (2019)
V Hernandez-Gordillo, et al. Mimicking the extracellular matrix with functionalized, metal-assembled collagen peptide scaffolds. Biomaterials. (2014-08)
Fiona Wegman, et al. Gene delivery of bone morphogenetic protein-2 plasmid DNA promotes bone formation in a large animal model. J Tissue Eng Regen Med. (2012-08)
You can visit "Citations Database" for more citations.
For more documents, please visit "Technical Support".
| Cat. No. A00613 | ||
|---|---|---|
| Size | Price | |
| 100 μg | $410.00 | |
