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Streptavidin Antibody, pAb, Rabbit

Synonyms Rabbit Anti-Streptavidin pAb; Anti Streptavidin
Description Streptavidin is a tetrameric protein from Streptomyces avidinii that binds very tightly to the vitamin biotin with a Kd of ≈10-14 mol/L. The high-affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.

GenScript Streptavidin Antibody is developed in rabbit using purified Streptavidin and highly purified from rabbit antiserum by protein G resin.
Host Species Rabbit
Conjugation Unconjugated
Immunogen Purified recombinant streptavidin
Purification Protein G chromatography

Subclass Rabbit IgG
Concentration 5 mg/ml, lyophilized in PBS, pH7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 5.0 mg/ml.
Specificity GenScript Streptavidin Antibody is specific to streptavidin.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.5-1 ug/ml
Dot blot: 0.5-1 ug/ml
Western blot: 0.5-1 ug/ml
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

Snyder H.,et al. Nutritional control of antibiotic resistance via an interface between the phosphotransferase system and a two-component signaling system.Antimicrob Agents Chemother.2013Nov;

Yonekita T.,et al. Development of a novel multiplex lateral flow assay using an antimicrobial peptide for the detection of Shiga toxin–producing Escherichia coli.J Microbiol Methods.2013Jun;93(3):251-6

Lorey S, et al. Novel Ubiquitin-derived High Affinity Binding Proteins with Tumor Targeting Properties. J Biol Chem. 2014Mar.;289(12):8493-8507

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Cat. No. A00621
5 mg $69.00
10 mg $120.00
24 hour