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Catalog Products » Antibodies » Epitope Tag Antibodies » Glu-Glu-tag Antibody, pAb, Rabbit

Glu-Glu-tag Antibody, pAb, Rabbit

Glu-Glu-tag Antibody, PAb, Rabbit

Western blot analysis of Glu-Glu-tagged fusion protein using Glu-Glu-tag Antibody, pAb, Rabbit (GenScript, A00627, 1 µg/ml)
The signal was developed with IRDye™ 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 52 kD
Observed Size: 52 kD

Glu-Glu-tag Antibody, PAb, Rabbit

Western blot analysis of Glu-Glu-tagged fusion protein using Glu-Glu-tag Antibody, pAb, Rabbit (GenScript, A00627, 1 µg/ml)
The signal was developed with IRDye™ 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 63 kD
Observed Size: 63 kD

Synonyms Rabbit Anti-Glu-Glu-tag pAb; Anti Glu-Glu-tag
Description Well-characterized antibodies for epitope tags consisting of short sequences are widely used in the study of protein expression in various systems.

The Glu-Glu-tag is an epitope tag consisting of a six-residue peptide, EYMPME, derived from the polyoma virus medium T antigen.

GenScript Rabbit Anti-Glu-Glu-tag Polyclonal Antibody is developed in rabbit hosts using a synthetic Glu-Glu-tag epitope peptide conjugated to KLH.
Host Species Rabbit
Conjugation Unconjugated
Immunogen Glu-Glu epitope tag peptide (EYMPME) conjugated to KLH
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to antibody concentration of 0.5 mg/ml.
Specificity GenScript Rabbit Anti-Glu-Glu--tag Polyclonal Antibody recognizes Glu-Glu tagged fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, detection method sensitivity, temperature, incubations length, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are starting recommendations for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 1-2 µg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

Glu-Glu-tag Antibody, PAb, Rabbit
Glu-Glu-tag Antibody, PAb, Rabbit

Western blot analysis of Glu-Glu-tagged fusion protein using Glu-Glu-tag Antibody, pAb, Rabbit (GenScript, A00627, 1 µg/ml)
The signal was developed with IRDye™ 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 52 kD
Observed Size: 52 kD

Glu-Glu-tag Antibody, PAb, Rabbit
Glu-Glu-tag Antibody, PAb, Rabbit

Western blot analysis of Glu-Glu-tagged fusion protein using Glu-Glu-tag Antibody, pAb, Rabbit (GenScript, A00627, 1 µg/ml)
The signal was developed with IRDye™ 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 63 kD
Observed Size: 63 kD


Nathan M. Beach.,et al. Chimeric Porcine Circoviruses (PCV) Containing Amino Acid Epitope Tags in the C Terminus of the Capsid Gene Are Infectious and Elicit both Anti-Epitope Tag Antibodies and Anti-PCV Type 2 Neutralizing Antibodies in Pigs.J Virol.2011May;85(9):4591 - 4595

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Cat. No. A00627
Size
Price
40 ug $99.00
100 ug $199.00
24 hour
 
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