Catalog Products » Antibodies » Epitope Tag Antibodies » AU1-tag Antibody, pAb, Rabbit

AU1-tag Antibody, pAb, Rabbit

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Lane 1-3: AU1-tag fusion protein in 293 cell lysate (≈ 52 kD)
Lane 4: Negative 293 cell lysate (GenScript, M0032)
Primary Antibody: 2 µg/ml Rabbit Anti-AU1-tag Polyclonal Antibody (GenScript, A00628)
Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000)
The signal was developed with LumiSensor^TM HRP Substrate Kit (GenScript, L00221V500)

Western blot analysis of AU1 tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-AU1-tag Polyclonal Antibody (GenScript, A00628, 1 µg/ml) The signal was developed with IRDy^TM 800 Conjugated Goat Anti-Rabbit IgG.

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Overview

Synonyms	Rabbit Anti-AU1-tag; Anti-AU1-tag
Description	Rabbit Anti-AU1-tag Polyclonal Antibody is purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 μg aliquot at a concentration of 1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide.
Host Species	Rabbit
Conjugation	Unconjugated
Immunogen	AU1 epitope tag peptide DTYRYI conjugated to KLH
Purification	Immunoaffinity chromatography

Properties

Subclass	Rabbit IgG
Concentration	1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide
Specificity	This Antibody recognizes AU1 tagged fusion proteins.
Storage	The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Note	Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage. Before use, centrifuge the vial briefly to bring down any liquid in the cap.

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-2.0 µg/ml
Other applications: user-optimized

Technical Manual

ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)