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Catalog Products » Antibodies » Epitope Tag Antibodies » HAT-tag Antibody, pAb, Rabbit

HAT-tag Antibody, pAb, Rabbit

HAT-tag Antibody, PAb, Rabbit

Loading: HAT-tag fusion protein expressed in E. coli cell lysate
Detection antibody: 0.5 μg/ml Rabbit Anti-HAT-tag Polyclonal Antibody (GenScript, A00636)
The result was developed with One-Step WesternTM Basic Kit (GenScript, L00204)

HAT-tag Antibody, PAb, Rabbit

Western blot analysis of HAT tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-HAT-tag Polyclonal Antibody (GenScript, A00636, 1 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

Synonyms Rabbit Anti-HAT-tag pAb;
Description GenScript Rabbit Anti-HAT-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 100 μg aliquot at a concentration of 0.5 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide before lyophilization.
Host Species Rabbit
Conjugation Unconjugated
Immunogen HAT epitope tag peptide KDHLIHNVHKEFHAHAHNK conjugated to KLH
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Specificity GenScript Rabbit Anti-HAT-tag Polyclonal Antibody specifically recognizes HAT tagged fusion proteins.
Storage The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western blot using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00204C is recommended. 5 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

HAT-tag Antibody, PAb, Rabbit
HAT-tag Antibody, PAb, Rabbit

Loading: HAT-tag fusion protein expressed in E. coli cell lysate
Detection antibody: 0.5 μg/ml Rabbit Anti-HAT-tag Polyclonal Antibody (GenScript, A00636)
The result was developed with One-Step WesternTM Basic Kit (GenScript, L00204)

HAT-tag Antibody, PAb, Rabbit
HAT-tag Antibody, PAb, Rabbit

Western blot analysis of HAT tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-HAT-tag Polyclonal Antibody (GenScript, A00636, 1 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.


Longwen Xiao.,et al. Apa Is A Trimeric Autotransporter Adhesin Of Actinobacillus Pleuropneumoniae Responsible For Autoagglutination And Host Cell Adherence.J Basic Microbiol.2011Dec;

Okada Y.,et al. Molecular characterization and expression analysis of chitinase from the Pacific oyster Crassostrea gigas.Comp Biochem Physiol B Biochem Mol Biol.2013Jun;165(2):83-9

Chandrasekera PC.,et al. Adenosine A(1) receptors heterodimerize with β(1)- and β(2)-adrenergic receptors creating novel receptor complexes with altered G protein coupling and signaling.Cell Signal.2013Jan;S0898-6568(12):00359-2

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Cat. No. A00636
Size
Price
100 ug $199.00
24 hour
 
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