HAT-tag Antibody, pAb, Rabbit Antibody

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Overview

Synonyms	Rabbit Anti-HAT-tag pAb;
Description	GenScript Rabbit Anti-HAT-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 100 μg aliquot at a concentration of 0.5 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide before lyophilization.
Host Species	Rabbit
Conjugation	Unconjugated
Immunogen	HAT epitope tag peptide KDHLIHNVHKEFHAHAHNK conjugated to KLH
Purification	Immunoaffinity chromatography

Properties

Subclass	Rabbit IgG
Concentration	0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Specificity	GenScript Rabbit Anti-HAT-tag Polyclonal Antibody specifically recognizes HAT tagged fusion proteins.
Storage	The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western blot using ONE-HOUR Western^TM Kit: For quick results, GenScript ONE-HOUR Western^TM Kit L00204C is recommended. 5 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Other applications: user-optimized

Technical Manual

ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

Examples

Loading: HAT-tag fusion protein expressed in E. coli cell lysate
Detection antibody: 0.5 μg/ml Rabbit Anti-HAT-tag Polyclonal Antibody (GenScript, A00636)
The result was developed with One-Step Western^TM Basic Kit (GenScript, L00204)

Western blot analysis of HAT tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-HAT-tag Polyclonal Antibody (GenScript, A00636, 1 µg/ml) The signal was developed with IRDye^TM 800 Conjugated Goat Anti-Rabbit IgG.

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Longwen Xiao.,et al. Apa Is A Trimeric Autotransporter Adhesin Of Actinobacillus Pleuropneumoniae Responsible For Autoagglutination And Host Cell Adherence.J Basic Microbiol.2011Dec;

Okada Y.,et al. Molecular characterization and expression analysis of chitinase from the Pacific oyster Crassostrea gigas.Comp Biochem Physiol B Biochem Mol Biol.2013Jun;165(2):83-9

Chandrasekera PC.,et al. Adenosine A(1) receptors heterodimerize with Î²(1)- and Î²(2)-adrenergic receptors creating novel receptor complexes with altered G protein coupling and signaling.Cell Signal.2013Jan;S0898-6568(12):00359-2

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