HAT-tag Antibody, pAb, Rabbit
Loading: HAT-tag fusion protein expressed in E. coli cell lysate
Detection antibody: 0.5 μg/ml Rabbit Anti-HAT-tag Polyclonal Antibody (GenScript, A00636)
The result was developed with One-Step WesternTM Basic Kit (GenScript, L00204)
Western blot analysis of HAT tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-HAT-tag Polyclonal Antibody (GenScript, A00636, 1 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
| Synonyms | Rabbit Anti-HAT-tag pAb; |
| Description | GenScript Rabbit Anti-HAT-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 100 μg aliquot at a concentration of 0.5 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide before lyophilization. |
| Host Species | Rabbit |
| Conjugation | Unconjugated |
| Immunogen | HAT epitope tag peptide KDHLIHNVHKEFHAHAHNK conjugated to KLH |
| Purification | Immunoaffinity chromatography |
| Subclass | Rabbit IgG |
| Concentration | 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
| Specificity | GenScript Rabbit Anti-HAT-tag Polyclonal Antibody specifically recognizes HAT tagged fusion proteins. |
| Storage | The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
| Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.05-0.2 µg/ml Western blot: 0.1-1.0 µg/ml Western blot using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00204C is recommended. 5 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane. Other applications: user-optimized |
Loading: HAT-tag fusion protein expressed in E. coli cell lysate
Detection antibody: 0.5 μg/ml Rabbit Anti-HAT-tag Polyclonal Antibody (GenScript, A00636)
The result was developed with One-Step WesternTM Basic Kit (GenScript, L00204)
Western blot analysis of HAT tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-HAT-tag Polyclonal Antibody (GenScript, A00636, 1 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
Chandrasekera PC.,et al. Adenosine A(1) receptors heterodimerize with β(1)- and β(2)-adrenergic receptors creating novel receptor complexes with altered G protein coupling and signaling.Cell Signal.2013Jan;S0898-6568(12):00359-2
Okada Y.,et al. Molecular characterization and expression analysis of chitinase from the Pacific oyster Crassostrea gigas.Comp Biochem Physiol B Biochem Mol Biol.2013Jun;165(2):83-9
Longwen Xiao.,et al. Apa Is A Trimeric Autotransporter Adhesin Of Actinobacillus Pleuropneumoniae Responsible For Autoagglutination And Host Cell Adherence.J Basic Microbiol.2011Dec;
You can visit "Citations Database" for more citations.
Okada Y.,et al. Molecular characterization and expression analysis of chitinase from the Pacific oyster Crassostrea gigas.Comp Biochem Physiol B Biochem Mol Biol.2013Jun;165(2):83-9
Longwen Xiao.,et al. Apa Is A Trimeric Autotransporter Adhesin Of Actinobacillus Pleuropneumoniae Responsible For Autoagglutination And Host Cell Adherence.J Basic Microbiol.2011Dec;
You can visit "Citations Database" for more citations.
For more documents, please visit "Technical Support".
| Cat. No. A00636 | ||
|---|---|---|
Size |
Price |
|
| 100 ug | $199.00 | |
