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Lane 1-3: DDDDK tag fusion protein in E. coli lysatesLane 4: E. coli lysates Negative ControlThe result was developed with One-Step WesternTM Complete Kit (Rabbit) (GenScript, L00204C)
Lane 1-3: DDDDK tag fusion protein in E. coli lysatesLane 4: E. coli lysates Negative ControlPrimary Antibody: 1 µg/ml Rabbit Anti-DDDDK-tag Polyclonal Antibody (GenScript, A00676)Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000)The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)
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