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Catalog Products » Antibodies » Epitope Tag Antibodies » RFP-tag Antibody, pAb, Rabbit

RFP-tag Antibody, pAb, Rabbit

RFP-tag Antibody, PAb, Rabbit

Loading: RFP-tag fusion protein expressed in E. coli cell lysate
Primary antibody: 1 μg/ml Rabbit Anti-RFP-tag Polyclonal Antibody (GenScript, A00682)
The result was developed with One-Step WesternTM Basic Kit (GenScript, L00204).

RFP-tag Antibody, PAb, Rabbit

Lane 1: Marker
Lane 2-5: 40 ng, 8 ng, 1.6 ng, 0.8 ng RFP-tag fusion protein expressed in E. coli cell lysate
Primary antibody: 1 μg/ml Rabbit Anti-RFP-tag Polyclonal Antibody (GenScript, A00682)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG

RFP-tag Antibody, PAb, Rabbit

Western blot analysis of red fluorescent protein variants (mCherry, tdTomato, DsRed, DsRed2, mOrange)
using RFP-tag Antibody, pAb, Rabbit (GenScript, A00682, 1 µg/ml)

The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

Specificity GenScript RFP-tag Antibody, pAb, Rabbit specifically reacts with fusion proteins containing RFP-tags and RFP variants, such as mCherry, tdTomato, DsRed, DsRed2, mOrange.The antibody does not react with GFP or GFP-tagged proteins.
Host Species Rabbit
Immunogen RFP epitope tag peptide VNGHEFEIEGEGEGR conjugated to KLH
Conjugate Unconjugated

Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.05-0.2 µg/ml
Western Blot 0.1-1.0 µg/ml
Western Blot 1:100-1:1,000
Other applications User-optimized

Form Lyophilized
Storage Buffer Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Storage Instructions The antibody is stable in lyophilized form if stored at -20°C or below.The reconstituted antibody can be stored for 2-3 weeks at 2-8°C.For long term storage, aliquot and store at -20°C or below.Avoid repeated freezing and thawing cycles.
Purification Affinity chromatography
Isotype Rabbit IgG
Clonality Polyclonal
Clone ID Not applicable

  • RFP-tag Antibody, PAb, Rabbit
  • RFP-tag Antibody, PAb, Rabbit

    Loading: RFP-tag fusion protein expressed in E. coli cell lysate
    Primary antibody: 1 μg/ml Rabbit Anti-RFP-tag Polyclonal Antibody (GenScript, A00682)
    The result was developed with One-Step WesternTM Basic Kit (GenScript, L00204).

  • RFP-tag Antibody, PAb, Rabbit
  • RFP-tag Antibody, PAb, Rabbit

    Lane 1: Marker
    Lane 2-5: 40 ng, 8 ng, 1.6 ng, 0.8 ng RFP-tag fusion protein expressed in E. coli cell lysate
    Primary antibody: 1 μg/ml Rabbit Anti-RFP-tag Polyclonal Antibody (GenScript, A00682)
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG

  • RFP-tag Antibody, PAb, Rabbit
  • RFP-tag Antibody, PAb, Rabbit

    Western blot analysis of red fluorescent protein variants (mCherry, tdTomato, DsRed, DsRed2, mOrange)
    using RFP-tag Antibody, pAb, Rabbit (GenScript, A00682, 1 µg/ml)

    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.


Target Background Well-characterizated antibodies against short-sequence epitope tags are common in the study of protein expression in systems.RFP-tags are composed of 15-residue peptides, VNGHEFEIEGEGEGR, derived from amino acids 22-36 of red fluorescent protein (RFP) from the Discosoma sea anemone.
Synonyms Rabbit Anti RFP-tag pAb;Anti RFP-tag pAb

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.






For more documents, please visit "Technical Support".

Cat. No. A00682
Size Price
40 μg $99.00