Catalog Products » Antibodies » Primary Antibodies » Control Antibodies » THE™ beta Actin Antibody, mAb, Mouse
THE™ Beta Actin Antibody, MAb, Mouse

Lane 1: 20 µg Hela cell lysate
Lane 2: 20 µg sp2/0 cell lysate
Lane 3: 20 µg goat muscle lysate
Lane 4: 20 µg rabbit muscle lysate
Lane 5: 20 µg chicken muscle lysate
Lane 6: 20 µg CHO cell lysate
Lane 7: 20 µg fish muscle lysate

Primary antibody: 1 µg/ml THETM Anti-β-actin Monoclonal Antibody (Mouse) (GenScript, A00702)

Secondary antibody: Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:20,000)

THE™ Beta Actin Antibody, MAb, Mouse

Primary antibody:
A. 0.1 μg/ml THETM Anti-β-actin Monoclonal Antibody (Mouse) (GenScript, A00702)
B. 0.1 μg/ml THETM Anti-β-actin Monoclonal Antibody (Mouse) (Company A,clone 8226)
Secondary antibody:
Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, 1:10,000, A00160)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)

THE™ Beta Actin Antibody, MAb, Mouse

Primary antibody:
A. 0.1 μg/ml THETM Anti-β-actin Monoclonal Antibody (Mouse) (GenScript, A00702)
B. 0.1 μg/ml THETM Anti-β-actin Monoclonal Antibody (Mouse) (Company A, clone 8226)
Secondary antibody:
Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, 1:10,000, A00160)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)

THE™ beta Actin Antibody, mAb, Mouse

THE™ beta Actin Antibody, mAb, Mouse reacts with mouse, rabbit, chicken, human, hamster, cow, goat, fish, and pig.It has not yet been tested in other species.
A00702
$200.00

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Overview
Specificity THE™ beta Actin Antibody, mAb, Mouse reacts with mouse, rabbit, chicken, human, hamster, cow, goat, fish, and pig.It has not yet been tested in other species.
Host Species Mouse
Immunogen A synthetic peptide DDDIAALVVDNGSG coupled to KLH
Species Reactivity Mouse, rabbit, chicken, human, hamster, cow, goat, fish, and pig
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.1-1.0 µg/ml
Western Blot 0.1-1.0 µg/ml

Properties
Form Lyophilized
Storage Buffer Lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide.Note: 100 µg, 200µg (GenScript, A00702), lyophilized with PBS, pH 7.4, containing 0.02% sodium azide, without BSA
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Storage Instructions The lyophilized product remains stable for up to 1 year at -20 °C from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Purification Protein A or G affinity column
Isotype Mouse IgG2a,κ
Clonality Monoclonal
Clone ID 2D1D10

Examples
  • THE™ Beta Actin Antibody, MAb, Mouse
  • THE™ Beta Actin Antibody, MAb, Mouse

    Lane 1: 20 µg Hela cell lysate
    Lane 2: 20 µg sp2/0 cell lysate
    Lane 3: 20 µg goat muscle lysate
    Lane 4: 20 µg rabbit muscle lysate
    Lane 5: 20 µg chicken muscle lysate
    Lane 6: 20 µg CHO cell lysate
    Lane 7: 20 µg fish muscle lysate

    Primary antibody: 1 µg/ml THETM Anti-β-actin Monoclonal Antibody (Mouse) (GenScript, A00702)

    Secondary antibody: Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:20,000)

  • THE™ Beta Actin Antibody, MAb, Mouse
  • THE™ Beta Actin Antibody, MAb, Mouse

    Primary antibody:
    A. 0.1 μg/ml THETM Anti-β-actin Monoclonal Antibody (Mouse) (GenScript, A00702)
    B. 0.1 μg/ml THETM Anti-β-actin Monoclonal Antibody (Mouse) (Company A,clone 8226)
    Secondary antibody:
    Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, 1:10,000, A00160)
    The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)

  • THE™ Beta Actin Antibody, MAb, Mouse
  • THE™ Beta Actin Antibody, MAb, Mouse

    Primary antibody:
    A. 0.1 μg/ml THETM Anti-β-actin Monoclonal Antibody (Mouse) (GenScript, A00702)
    B. 0.1 μg/ml THETM Anti-β-actin Monoclonal Antibody (Mouse) (Company A, clone 8226)
    Secondary antibody:
    Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, 1:10,000, A00160)
    The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)


Background
Target Background β-actin is one of the six different actin isoforms that have been identified.The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties.As a consequence, it has been difficult to produce potent antisera against this protein.Therefore, the availability of monoclonal antibodies to ß-actin provides a useful and specific tool in the study of the intracellular distribution of β-actin and the static and dynamic aspects of the cytoskeleton.
Synonyms cytoplasmic 1 antibody;

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


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